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Q9GLG1

- CAN2_MACFA

UniProt

Q9GLG1 - CAN2_MACFA

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Protein
Calpain-2 catalytic subunit
Gene
CAPN2
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions By similarity.

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Calcium 3; via carbonyl oxygen By similarity
Metal bindingi91 – 911Calcium 3; via carbonyl oxygen By similarity
Metal bindingi96 – 961Calcium 3 By similarity
Active sitei105 – 1051 By similarity
Metal bindingi175 – 1751Calcium 3 By similarity
Metal bindingi229 – 2291Calcium 2 By similarity
Metal bindingi230 – 2301Calcium 2 By similarity
Active sitei262 – 2621 By similarity
Active sitei286 – 2861 By similarity
Metal bindingi292 – 2921Calcium 4 By similarity
Metal bindingi299 – 2991Calcium 4 By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygen By similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygen By similarity
Metal bindingi545 – 5451Calcium 5 By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygen By similarity
Metal bindingi552 – 5521Calcium 5 By similarity
Metal bindingi585 – 5851Calcium 6 By similarity
Metal bindingi587 – 5871Calcium 6 By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygen By similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygen By similarity
Metal bindingi596 – 5961Calcium 6 By similarity
Metal bindingi615 – 6151Calcium 7 By similarity
Metal bindingi617 – 6171Calcium 7 By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygen By similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygen By similarity
Metal bindingi626 – 6261Calcium 7 By similarity
Metal bindingi658 – 6581Calcium 1 By similarity
Metal bindingi661 – 6611Calcium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121
Add
BLAST
Calcium bindingi615 – 626122
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 3121.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Propeptidei2 – 1918Anchors to the small subunit Reviewed prediction
PRO_0000026489Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunit
PRO_0000026490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9GLG1.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliQ9GLG1.
SMRiQ9GLG1. Positions 2-700.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalytic
Add
BLAST
Domaini572 – 60534EF-hand 1
Add
BLAST
Domaini602 – 63736EF-hand 2
Add
BLAST
Domaini667 – 70034EF-hand 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain III
Add
BLAST
Regioni515 – 52915Linker
Add
BLAST
Regioni530 – 700171Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 3 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
KOiK03853.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GLG1-1 [UniParc]FASTAAdd to Basket

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MAGIAAKLVK DREAAEGLGS HERAIKYLNQ DYEALRNECL EAGTLFQDPS    50
FPAIPSALGF KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG 100
ALGDCWLLAA IASLTLNEEI LARVVPLNQS FQENYAGIFH FQFWQYGEWV 150
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
TEGFEDFTGG IAEWYELKKP PPNLFKIIQK ALQKGSLLGC SIDITSAADS 250
EAITYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGE VEWTGRWNDN 300
CPSWNTIDPE ERERLTRRHE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTS 350
DTYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED 400
GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL SGQTNIHLSK 450
NFFLTNRARE RSDTFINLRE VLNRFKLPPG EYILVPSTFE PNKDGDFCIR 500
VFSEKKADYQ AVDDEIEANL EEFDISEDDI DDGFRRLFAQ LAGEDAEISA 550
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 600
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF 650
ADDQLIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIELD LISWLCFSVL 700
Length:700
Mass (Da):80,026
Last modified:January 23, 2007 - v3
Checksum:iDCEE16214F05057C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284441 mRNA. Translation: AAG22771.1.
RefSeqiNP_001274615.1. NM_001287686.1.
UniGeneiMfa.5652.

Genome annotation databases

GeneIDi102122692.
KEGGimcf:102122692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284441 mRNA. Translation: AAG22771.1 .
RefSeqi NP_001274615.1. NM_001287686.1.
UniGenei Mfa.5652.

3D structure databases

ProteinModelPortali Q9GLG1.
SMRi Q9GLG1. Positions 2-700.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PRIDEi Q9GLG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102122692.
KEGGi mcf:102122692.

Organism-specific databases

CTDi 824.

Phylogenomic databases

HOVERGENi HBG012645.
KOi K03853.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 3121.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues."
    Nakajima T., Fukiage C., Azuma M., Ma H., Shearer T.R.
    Biochim. Biophys. Acta 1519:55-64(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.

Entry informationi

Entry nameiCAN2_MACFA
AccessioniPrimary (citable) accession number: Q9GLG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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