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Q9GLG1

- CAN2_MACFA

UniProt

Q9GLG1 - CAN2_MACFA

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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi96 – 961Calcium 3By similarity
Active sitei105 – 1051By similarity
Metal bindingi175 – 1751Calcium 3By similarity
Metal bindingi229 – 2291Calcium 2By similarity
Metal bindingi230 – 2301Calcium 2By similarity
Active sitei262 – 2621By similarity
Active sitei286 – 2861By similarity
Metal bindingi292 – 2921Calcium 4By similarity
Metal bindingi299 – 2991Calcium 4By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi545 – 5451Calcium 5By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi552 – 5521Calcium 5By similarity
Metal bindingi585 – 5851Calcium 6By similarity
Metal bindingi587 – 5871Calcium 6By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi596 – 5961Calcium 6By similarity
Metal bindingi615 – 6151Calcium 7By similarity
Metal bindingi617 – 6171Calcium 7By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi626 – 6261Calcium 7By similarity
Metal bindingi658 – 6581Calcium 1By similarity
Metal bindingi661 – 6611Calcium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121Add
BLAST
Calcium bindingi615 – 626122Add
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. proteolysis Source: UniProtKB
  3. regulation of cytoskeleton organization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 3121.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026489Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9GLG1.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliQ9GLG1.
SMRiQ9GLG1. Positions 2-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain IIIAdd
BLAST
Regioni515 – 52915LinkerAdd
BLAST
Regioni530 – 700171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
KOiK03853.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GLG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGIAAKLVK DREAAEGLGS HERAIKYLNQ DYEALRNECL EAGTLFQDPS
60 70 80 90 100
FPAIPSALGF KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPLNQS FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT
210 220 230 240 250
TEGFEDFTGG IAEWYELKKP PPNLFKIIQK ALQKGSLLGC SIDITSAADS
260 270 280 290 300
EAITYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGE VEWTGRWNDN
310 320 330 340 350
CPSWNTIDPE ERERLTRRHE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTS
360 370 380 390 400
DTYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED
410 420 430 440 450
GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL SGQTNIHLSK
460 470 480 490 500
NFFLTNRARE RSDTFINLRE VLNRFKLPPG EYILVPSTFE PNKDGDFCIR
510 520 530 540 550
VFSEKKADYQ AVDDEIEANL EEFDISEDDI DDGFRRLFAQ LAGEDAEISA
560 570 580 590 600
FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF
660 670 680 690 700
ADDQLIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIELD LISWLCFSVL
Length:700
Mass (Da):80,026
Last modified:January 23, 2007 - v3
Checksum:iDCEE16214F05057C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284441 mRNA. Translation: AAG22771.1.
RefSeqiNP_001274615.1. NM_001287686.1.
UniGeneiMfa.5652.

Genome annotation databases

GeneIDi102122692.
KEGGimcf:102122692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284441 mRNA. Translation: AAG22771.1 .
RefSeqi NP_001274615.1. NM_001287686.1.
UniGenei Mfa.5652.

3D structure databases

ProteinModelPortali Q9GLG1.
SMRi Q9GLG1. Positions 2-700.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PRIDEi Q9GLG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102122692.
KEGGi mcf:102122692.

Organism-specific databases

CTDi 824.

Phylogenomic databases

HOVERGENi HBG012645.
KOi K03853.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 3121.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues."
    Nakajima T., Fukiage C., Azuma M., Ma H., Shearer T.R.
    Biochim. Biophys. Acta 1519:55-64(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.

Entry informationi

Entry nameiCAN2_MACFA
AccessioniPrimary (citable) accession number: Q9GLG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3