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Q9GLG1

- CAN2_MACFA

UniProt

Q9GLG1 - CAN2_MACFA

Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi89 – 891Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi96 – 961Calcium 3By similarity
    Active sitei105 – 1051By similarity
    Metal bindingi175 – 1751Calcium 3By similarity
    Metal bindingi229 – 2291Calcium 2By similarity
    Metal bindingi230 – 2301Calcium 2By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity
    Metal bindingi292 – 2921Calcium 4By similarity
    Metal bindingi299 – 2991Calcium 4By similarity
    Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi545 – 5451Calcium 5By similarity
    Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi552 – 5521Calcium 5By similarity
    Metal bindingi585 – 5851Calcium 6By similarity
    Metal bindingi587 – 5871Calcium 6By similarity
    Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi596 – 5961Calcium 6By similarity
    Metal bindingi615 – 6151Calcium 7By similarity
    Metal bindingi617 – 6171Calcium 7By similarity
    Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi626 – 6261Calcium 7By similarity
    Metal bindingi658 – 6581Calcium 1By similarity
    Metal bindingi661 – 6611Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi585 – 596121Add
    BLAST
    Calcium bindingi615 – 626122Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: UniProtKB
    2. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.53. 3121.

    Protein family/group databases

    MEROPSiC02.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:CAPN2
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. dendrite Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026489Add
    BLAST
    Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ9GLG1.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Structurei

    3D structure databases

    ProteinModelPortaliQ9GLG1.
    SMRiQ9GLG1. Positions 2-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni345 – 514170Domain IIIAdd
    BLAST
    Regioni515 – 52915LinkerAdd
    BLAST
    Regioni530 – 700171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG012645.
    KOiK03853.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9GLG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGIAAKLVK DREAAEGLGS HERAIKYLNQ DYEALRNECL EAGTLFQDPS    50
    FPAIPSALGF KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG 100
    ALGDCWLLAA IASLTLNEEI LARVVPLNQS FQENYAGIFH FQFWQYGEWV 150
    EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT 200
    TEGFEDFTGG IAEWYELKKP PPNLFKIIQK ALQKGSLLGC SIDITSAADS 250
    EAITYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGE VEWTGRWNDN 300
    CPSWNTIDPE ERERLTRRHE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTS 350
    DTYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED 400
    GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL SGQTNIHLSK 450
    NFFLTNRARE RSDTFINLRE VLNRFKLPPG EYILVPSTFE PNKDGDFCIR 500
    VFSEKKADYQ AVDDEIEANL EEFDISEDDI DDGFRRLFAQ LAGEDAEISA 550
    FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL 600
    WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF 650
    ADDQLIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIELD LISWLCFSVL 700
    Length:700
    Mass (Da):80,026
    Last modified:January 23, 2007 - v3
    Checksum:iDCEE16214F05057C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF284441 mRNA. Translation: AAG22771.1.
    RefSeqiNP_001274615.1. NM_001287686.1.
    UniGeneiMfa.5652.

    Genome annotation databases

    GeneIDi102122692.
    KEGGimcf:102122692.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF284441 mRNA. Translation: AAG22771.1 .
    RefSeqi NP_001274615.1. NM_001287686.1.
    UniGenei Mfa.5652.

    3D structure databases

    ProteinModelPortali Q9GLG1.
    SMRi Q9GLG1. Positions 2-700.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C02.002.

    Proteomic databases

    PRIDEi Q9GLG1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102122692.
    KEGGi mcf:102122692.

    Organism-specific databases

    CTDi 824.

    Phylogenomic databases

    HOVERGENi HBG012645.
    KOi K03853.

    Enzyme and pathway databases

    BRENDAi 3.4.22.53. 3121.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues."
      Nakajima T., Fukiage C., Azuma M., Ma H., Shearer T.R.
      Biochim. Biophys. Acta 1519:55-64(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.

    Entry informationi

    Entry nameiCAN2_MACFA
    AccessioniPrimary (citable) accession number: Q9GLG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3