Reviewed, UniProtKB/Swiss-Prot Q9GLF6 (OREX_CANFA)

Names and origin

Protein names

Recommended name:
    Orexin
Alternative name(s):
    Hypocretin
      Short name=Hcrt
Cleaved into the following 2 chains:
    1- Recommended name:
            Orexin-A
        Alternative name(s):
            Hypocretin-1
              Short name=Hcrt1
    2- Recommended name:
            Orexin-B
        Alternative name(s):
            Hypocretin-2
              Short name=Hcrt2

Gene names

Name:	HCRT
Synonyms:	OX, PPOX

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
Subcellular location	Rough endoplasmic reticulum By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses By similarity.
Post-translational modification	Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Sequence similarities	Belongs to the orexin family.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Endoplasmic reticulum Synapse
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues Disulfide bond Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	feeding behavior Inferred from electronic annotation. Source: InterPro neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell rough endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	By similarity
	Peptide	33 – 65	33	Orexin-A		PRO_0000020258
	Peptide	69 – 96	28	Orexin-B		PRO_0000020259
	Propeptide	97 – 130	34			PRO_0000020260
Amino acid modifications
	Modified residue	33	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	65	1	Leucine amide By similarity
	Modified residue	96	1	Methionine amide By similarity
	Disulfide bond	38 ↔ 44		By similarity
	Disulfide bond	39 ↔ 46		By similarity
Natural variations
	Natural variant	30	1	A → T

Sequences

	Sequence		Length	Mass (Da)
	Q9GLF6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2BF59D4C1E422DF3	FASTA	130	13,328
	10 20 30 40 50 60 MNPPSTKVPW AAVTLLLLLL LPPALLSPGA AAQPLPDCCR QKTCSCRLYE LLHGAGNHAA 70 80 90 100 110 120 GILTLGKRRP GPPGLQGRLQ RLLQASGNHA AGILTMGRRA GAEPAPRPCP GRRCPVVAVP 130 SAAPGGRSGV

References

[1]	"Identification and functional analysis of mutations in the hypocretin (orexin) genes of narcoleptic canines." Hungs M., Fan J., Lin L., Lin X., Maki R.A., Mignot E. Genome Res. 11:531-539(2001) [PubMed: 11282968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-30.
[2]	"Hypocretin/orexin, sleep and narcolepsy." Hungs M., Mignot E. Bioessays 23:397-408(2001) [PubMed: 11340621] [Abstract] Cited for: REVIEW.
[3]	"To eat or to sleep? Orexin in the regulation of feeding and wakefulness." Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M. Annu. Rev. Neurosci. 24:429-458(2001) [PubMed: 11283317] [Abstract] Cited for: REVIEW.

Web resources

Protein Spotlight

Qui dort dine - Issue 15 of October 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF285110 Genomic DNA. Translation: AAG13965.1.
RefSeq	NP_001029166.1.
UniGene	Cfa.24652
3D structure databases
SMR	Q9GLF6. Positions 33-65, 71-96.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9GLF6.
Genome annotation databases
Ensembl	ENSCAFT00000024739; ENSCAFP00000022949; ENSCAFG00000015615; Canis familiaris. [Genome view]
GeneID	607641.
KEGG	cfa:607641.
Organism-specific databases
CTD	607641.
Phylogenomic databases
eggNOG	maNOG21262.
HOVERGEN	Q9GLF6.
InParanoid	Q9GLF6.
OMA	PCPGRRC.
OrthoDB	EOG96X1TV.
Family and domain databases
InterPro	IPR001704. Orexin. [Graphical view]
PANTHER	PTHR15173. Orexin. 1 hit.
Pfam	PF02072. Orexin. 1 hit. [Graphical view]
PRINTS	PR01091. OREXINPP.
ProtoNet	Search...

Entry information

Entry name

OREX_CANFA

Accession

Primary (citable) accession number: Q9GLF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)