Reviewed, UniProtKB/Swiss-Prot Q9GLE4 (MMP14_BOVIN)

Names and origin

Protein names

Recommended name:
    Matrix metalloproteinase-14
      Short name=MMP-14
    EC=3.4.24.80
Alternative name(s):
    Membrane-type matrix metalloproteinase 1
      Short name=MT-MMP 1
      Short name=MTMMP1
    Membrane-type-1 matrix metalloproteinase
      Short name=MT1-MMP
      Short name=MT1MMP

Gene names

Name:

MMP14

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endopeptidase that degrades various components of the extracellular matrix, such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development By similarity.
Catalytic activity	Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
Cofactor	Binds 1 zinc ion per subunit By similarity. Calcium By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Melanosome By similarity.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	branching morphogenesis of a tube Inferred from sequence or structural similarity. Source: AgBase cell migration Inferred from sequence or structural similarity. Source: AgBase lung development Inferred from sequence or structural similarity. Source: AgBase proteolysis Inferred from electronic annotation. Source: InterPro zymogen activation Inferred from sequence or structural similarity. Source: AgBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 110	90	Activation peptide		PRO_0000289295
	Chain	111 – 582	472	Matrix metalloproteinase-14		PRO_0000289296
Regions
	Topological domain	111 – 541	431	Extracellular Potential
	Transmembrane	542 – 562	21	Potential
	Topological domain	563 – 582	20	Cytoplasmic Potential
	Domain	322 – 365	44	Hemopexin-like 1
	Domain	367 – 411	45	Hemopexin-like 2
	Domain	414 – 460	47	Hemopexin-like 3
	Domain	462 – 507	46	Hemopexin-like 4
	Motif	90 – 97	8	Cysteine switch By similarity
Sites
	Active site	239	1	By similarity
	Metal binding	92	1	Zinc; in inhibited form By similarity
	Metal binding	238	1	Zinc; catalytic By similarity
	Metal binding	242	1	Zinc; catalytic By similarity
	Metal binding	248	1	Zinc; catalytic By similarity
Amino acid modifications
	Disulfide bond	318 ↔ 507		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GLE4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 65174CE65D4040E1	FASTA	582	65,883
	10 20 30 40 50 60 MSPAPRPACS LLLPVLTLAS ALASLSSAQS SFSPEAWLQQ HGYLPPGDLR THTQRSPQSL 70 80 90 100 110 120 SAAIAAMQRF YGLRVTGKAD ADTMKAMRRP RCGVPDKFGA EIKANVRRKR YAIQGLKWQH 130 140 150 160 170 180 NEITFCIQNY TPNVGEYATF EAIRKAFRVW ESATPLRFRE VPYAYIREGH EKQADIMIFF 190 200 210 220 230 240 AEGFHGDSTP FDGEGGFLAH AYFPGPNIGG DTHFDSAEPW TVRNEDLNGN DIFLVAVHEL 250 260 270 280 290 300 GHALGLEHSN DPSAIMAPFY QWMDTENFVL PDDDRRGIQQ LYGSKSGSPT KMPPQPRTTS 310 320 330 340 350 360 RPSVPDKPKN PTYGPNICDG NFDTVAMLRG EMFVFKERWF WRVRKNQVMD GYPMPIGQFW 370 380 390 400 410 420 RGLPASINTA YERKDGKFVF FKGDKHWVFD EASLEPGYPK HIKELGRGLP TDRIDAALFW 430 440 450 460 470 480 MPNGKTYFFR GNKYYRFNEE LRIVESEYPK NIKVWEGIPE SPRGSFMGSD EVFTYFYKGN 490 500 510 520 530 540 KYWKFNNQKL KVEPGYPKSA LRDWMGCPSS GGQPDEGTEE ETEVIIIEVD EEGSGAVSAA 550 560 570 580 AVVLPVLLLL LVLAVGLAVF FFRRHGTPKR LLYCQRSLLD KV

References

[1]	"Molecular cloning and biological characterization of bovine membrane-type matrix metalloprotease 1 (bMT1-MMP)." Zhang B., Yan L., Moses M.A., Fang J., Miao H., Tsang P. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF290429 mRNA. Translation: AAG28170.1.
IPI	IPI00692043.
RefSeq	NP_776815.1.
UniGene	Bt.7046
3D structure databases
HSSP	HSSP built from PDB template 1BQQ based on UniProtKB P50281.
SMR	Q9GLE4. Positions 113-286, 315-507.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9GLE4.
Protein family/group databases
MEROPS	M10.014.
Genome annotation databases
Ensembl	ENSBTAT00000019744; ENSBTAP00000019744; ENSBTAG00000014824; Bos taurus. [Genome view]
GeneID	281915.
KEGG	bta:281915.
Organism-specific databases
CTD	281915.
Phylogenomic databases
HOVERGEN	Q9GLE4.
InParanoid	Q9GLE4.
Enzyme and pathway databases
BRENDA	3.4.24.80. 251.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 1 hit.
Pfam	PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMP14_BOVIN

Accession

Primary (citable) accession number: Q9GLE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)