Reviewed, UniProtKB/Swiss-Prot Q9GL27 (BRAC_CANFA)

Names and origin

Protein names

Recommended name:
    Brachyury protein
      Short name=T protein

Gene names

Name:

T

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus.
Polymorphism	A mutation in position 63 is responsible for a short-tail trait in a bob-tailed dog. The homozygous mutation is lethal.
Sequence similarities	Contains 1 T-box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Brachyury protein		PRO_0000184413
Regions
	DNA binding	51 – 219	169	T-box
Natural variations
	Natural variant	63	1	I → M in a bob-tailed dog; alters the ability of the protein to bind to its consensus DNA target. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9GL27-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CDCDB4A53A395AE2	FASTA	435	47,148
	10 20 30 40 50 60 MSSPGAESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEDSE LWLRFKELTN 70 80 90 100 110 120 EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP 130 140 150 160 170 180 SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGAQR 190 200 210 220 230 240 MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKDMM EEPGDSQQSG 250 260 270 280 290 300 YSQWGWLIPG TSTLCPPATP HPQFGGPLSL PSTHGCERYP ALRNHRPSPY PSPYAHRNNS 310 320 330 340 350 360 PTYSDNPSAC LSMLQSHDSW PSLGTPAHTS MLPMSHSAGP PTGSSQYPSL WSVSNGTITP 370 380 390 400 410 420 GAQPAGMSNG LGAQFFRGSS AHGAPLGHAV PAPSASGSPL YEGAPTATDV PDSQYDASAQ 430 ARLIASWTPV SPPSM

References

[1]

"Canine homolog of the T-box transcription factor T; failure of the protein to bind to its DNA target leads to a short-tail phenotype." Haworth K.E., Putt W., Cattanach B., Breen M., Binns M., Lingaas F., Edwards Y.H. Mamm. Genome 12:212-218(2001) [PubMed: 11252170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245513 mRNA. Translation: CAC10530.1.
RefSeq	NP_001003092.1.
UniGene	Cfa.3518
3D structure databases
HSSP	HSSP built from PDB template 1XBR based on UniProtKB P24781.
SMR	Q9GL27. Positions 41-224.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000000807. Canis familiaris. [Contig view]
GeneID	403653.
KEGG	cfa:403653.
Phylogenomic databases
HOVERGEN	Q9GL27.
Family and domain databases
InterPro	IPR002070. TF_Brachyury. IPR001699. TF_T-box. IPR018186. TF_T-box_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.820. TF_T-box. 1 hit.
PANTHER	PTHR11267. TF_T-box. 1 hit.
Pfam	PF00907. T-box. 1 hit. [Graphical view]
PRINTS	PR00938. BRACHYURY. PR00937. TBOX.
SMART	SM00425. TBOX. 1 hit. [Graphical view]
PROSITE	PS01283. TBOX_1. 1 hit. PS01264. TBOX_2. 1 hit. PS50252. TBOX_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRAC_CANFA

Accession

Primary (citable) accession number: Q9GL27

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)