Q9GL27 (BRAC_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brachyury protein

Alternative name(s):
Protein T

Gene names

Name:

T

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus.
Polymorphism	A mutation in position 63 is responsible for a short-tail trait in a bob-tailed dog. The homozygous mutation is lethal.
Sequence similarities	Contains 1 T-box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from electronic annotation. Source: Compara SMAD protein signal transduction Inferred from electronic annotation. Source: Compara bone morphogenesis Inferred from electronic annotation. Source: Compara canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara cardiac muscle cell differentiation Inferred from electronic annotation. Source: Compara determination of heart left/right asymmetry Inferred from electronic annotation. Source: Compara embryonic skeletal system development Inferred from electronic annotation. Source: Compara heart morphogenesis Inferred from electronic annotation. Source: Compara mesoderm migration involved in gastrulation Inferred from electronic annotation. Source: Compara neural plate morphogenesis Inferred from electronic annotation. Source: Compara neural tube closure Inferred from electronic annotation. Source: Compara notochord formation Inferred from electronic annotation. Source: Compara penetration of zona pellucida Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Inferred from electronic annotation. Source: Compara post-anal tail morphogenesis Inferred from electronic annotation. Source: Compara signal transduction involved in regulation of gene expression Inferred from electronic annotation. Source: Compara somitogenesis Inferred from electronic annotation. Source: Compara vasculogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nuclear chromatin Inferred from electronic annotation. Source: Compara
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding Inferred from electronic annotation. Source: Compara RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Brachyury protein		PRO_0000184413
Regions
	DNA binding	51 – 219	169	T-box
Natural variations
	Natural variant	63	1	I → M in a bob-tailed dog; alters the ability of the protein to bind to its consensus DNA target. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9GL27 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CDCDB4A53A395AE2	FASTA	435	47,148
	10 20 30 40 50 60 MSSPGAESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEDSE LWLRFKELTN 70 80 90 100 110 120 EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP 130 140 150 160 170 180 SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGAQR 190 200 210 220 230 240 MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKDMM EEPGDSQQSG 250 260 270 280 290 300 YSQWGWLIPG TSTLCPPATP HPQFGGPLSL PSTHGCERYP ALRNHRPSPY PSPYAHRNNS 310 320 330 340 350 360 PTYSDNPSAC LSMLQSHDSW PSLGTPAHTS MLPMSHSAGP PTGSSQYPSL WSVSNGTITP 370 380 390 400 410 420 GAQPAGMSNG LGAQFFRGSS AHGAPLGHAV PAPSASGSPL YEGAPTATDV PDSQYDASAQ 430 ARLIASWTPV SPPSM

References

[1]

"Canine homolog of the T-box transcription factor T; failure of the protein to bind to its DNA target leads to a short-tail phenotype." Haworth K.E., Putt W., Cattanach B., Breen M., Binns M., Lingaas F., Edwards Y.H. Mamm. Genome 12:212-218(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-63.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ245513 mRNA. Translation: CAC10530.1.
RefSeq	NP_001003092.1. NM_001003092.1.
UniGene	Cfa.3518.
3D structure databases
ProteinModelPortal	Q9GL27.
SMR	Q9GL27. Positions 41-224.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000001265; ENSCAFP00000001157; ENSCAFG00000000807.
GeneID	403653.
KEGG	cfa:403653.
Organism-specific databases
CTD	6862.
Phylogenomic databases
eggNOG	NOG257463.
GeneTree	ENSGT00690000101827.
HOGENOM	HOG000063663.
HOVERGEN	HBG004599.
InParanoid	Q9GL27.
KO	K10172.
OMA	QFFRGSP.
OrthoDB	EOG4FXR7S.
Family and domain databases
Gene3D	2.60.40.820. 1 hit.
InterPro	IPR008967. p53-like_TF_DNA-bd. IPR002070. TF_Brachyury. IPR001699. TF_T-box. IPR018186. TF_T-box_CS. [Graphical view]
PANTHER	PTHR11267. PTHR11267. 1 hit.
Pfam	PF00907. T-box. 1 hit. [Graphical view]
PRINTS	PR00938. BRACHYURY. PR00937. TBOX.
SMART	SM00425. TBOX. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS01283. TBOX_1. 1 hit. PS01264. TBOX_2. 1 hit. PS50252. TBOX_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817158.

Entry information

Entry name

BRAC_CANFA

Accession

Primary (citable) accession number: Q9GL27

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program