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Protein

Brachyury protein

Gene

T

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi51 – 219169T-boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  2. RNA polymerase II distal enhancer sequence-specific DNA binding Source: Ensembl
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: Ensembl
  4. RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Source: Ensembl

GO - Biological processi

  1. BMP signaling pathway Source: Ensembl
  2. bone morphogenesis Source: Ensembl
  3. canonical Wnt signaling pathway Source: Ensembl
  4. determination of heart left/right asymmetry Source: Ensembl
  5. embryonic skeletal system development Source: Ensembl
  6. heart morphogenesis Source: Ensembl
  7. mesoderm migration involved in gastrulation Source: Ensembl
  8. neural plate morphogenesis Source: Ensembl
  9. neural tube closure Source: Ensembl
  10. notochord formation Source: Ensembl
  11. penetration of zona pellucida Source: Ensembl
  12. positive regulation of cell proliferation Source: Ensembl
  13. positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: Ensembl
  14. post-anal tail morphogenesis Source: Ensembl
  15. signal transduction involved in regulation of gene expression Source: Ensembl
  16. SMAD protein signal transduction Source: Ensembl
  17. somitogenesis Source: Ensembl
  18. vasculogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Brachyury protein
Alternative name(s):
Protein T
Gene namesi
Name:T
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Chromosome 1

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nuclear chromatin Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Brachyury proteinPRO_0000184413Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001157.

Structurei

3D structure databases

ProteinModelPortaliQ9GL27.
SMRiQ9GL27. Positions 41-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257463.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000063663.
HOVERGENiHBG004599.
InParanoidiQ9GL27.
KOiK10172.
OMAiQFFRGSP.
OrthoDBiEOG7GN2PM.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00938. BRACHYURY.
PR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GL27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPGAESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEDSE
60 70 80 90 100
LWLRFKELTN EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH
110 120 130 140 150
RWKYVNGEWV PGGKPEPQAP SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN
160 170 180 190 200
KLNGGGQIML NSLHKYEPRI HIVRVGGAQR MITSHCFPET QFIAVTAYQN
210 220 230 240 250
EEITALKIKY NPFAKAFLDA KERSDHKDMM EEPGDSQQSG YSQWGWLIPG
260 270 280 290 300
TSTLCPPATP HPQFGGPLSL PSTHGCERYP ALRNHRPSPY PSPYAHRNNS
310 320 330 340 350
PTYSDNPSAC LSMLQSHDSW PSLGTPAHTS MLPMSHSAGP PTGSSQYPSL
360 370 380 390 400
WSVSNGTITP GAQPAGMSNG LGAQFFRGSS AHGAPLGHAV PAPSASGSPL
410 420 430
YEGAPTATDV PDSQYDASAQ ARLIASWTPV SPPSM
Length:435
Mass (Da):47,148
Last modified:March 1, 2001 - v1
Checksum:iCDCDB4A53A395AE2
GO

Polymorphismi

A mutation in position 63 is responsible for a short-tail trait in a bob-tailed dog. The homozygous mutation is lethal.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti63 – 631I → M in a bob-tailed dog; alters the ability of the protein to bind to its consensus DNA target. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245513 mRNA. Translation: CAC10530.1.
RefSeqiNP_001003092.1. NM_001003092.1.
UniGeneiCfa.3518.

Genome annotation databases

EnsembliENSCAFT00000001265; ENSCAFP00000001157; ENSCAFG00000000807.
GeneIDi403653.
KEGGicfa:403653.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245513 mRNA. Translation: CAC10530.1.
RefSeqiNP_001003092.1. NM_001003092.1.
UniGeneiCfa.3518.

3D structure databases

ProteinModelPortaliQ9GL27.
SMRiQ9GL27. Positions 41-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000001265; ENSCAFP00000001157; ENSCAFG00000000807.
GeneIDi403653.
KEGGicfa:403653.

Organism-specific databases

CTDi6862.

Phylogenomic databases

eggNOGiNOG257463.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000063663.
HOVERGENiHBG004599.
InParanoidiQ9GL27.
KOiK10172.
OMAiQFFRGSP.
OrthoDBiEOG7GN2PM.
TreeFamiTF106341.

Miscellaneous databases

NextBioi20817158.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00938. BRACHYURY.
PR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Canine homolog of the T-box transcription factor T; failure of the protein to bind to its DNA target leads to a short-tail phenotype."
    Haworth K.E., Putt W., Cattanach B., Breen M., Binns M., Lingaas F., Edwards Y.H.
    Mamm. Genome 12:212-218(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-63.

Entry informationi

Entry nameiBRAC_CANFA
AccessioniPrimary (citable) accession number: Q9GL27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.