Reviewed, UniProtKB/Swiss-Prot Q9GL24 (CATL1_CANFA)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL1
Synonyms:	CTSL

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes By similarity.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 117	100	Activation peptide By similarity		PRO_0000026240
	Chain	118 – 289	172	Cathepsin L1 heavy chain		PRO_0000026241
	Propeptide	290 – 291	2	By similarity		PRO_0000026242
	Chain	292 – 333	42	Cathepsin L1 light chain		PRO_0000026243
Sites
	Active site	138	1	By similarity
	Active site	277	1	By similarity
	Active site	300	1	By similarity
Amino acid modifications
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	135 ↔ 178		By similarity
	Disulfide bond	169 ↔ 212		By similarity
	Disulfide bond	270 ↔ 322		Interchain (between heavy and light chains) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GL24-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 033096E72B791D1F	FASTA	333	37,383
	10 20 30 40 50 60 MNPSLFLTAL CLGIASAAPK FDQSLNAQWY QWKATHRRLY GMNEEGWRRA VWEKNMKMIE 70 80 90 100 110 120 LHNREYSQGK HGFTMAMNAF GDMTNEEFRQ VMNGFQNQKH KKGKMFQEPL FAEIPKSVDW 130 140 150 160 170 180 REKGYVTPVK NQGQCGSCWA FSATGALEGQ MFRKTGKLVS LSEQNLVDCS RAQGNEGCNG 190 200 210 220 230 240 GLMDNAFRYV KDNGGLDSEE SYPYLGRDTE TCNYKPECSA ANDTGFVDLP QREKALMKAV 250 260 270 280 290 300 ATLGPISVAI DAGHQSFQFY KSGIYFDPDC SSKDLDHGVL VVGYGFEGTD SNNKFWIVKN 310 320 330 SWGPEWGWNG YVKMAKDQNN HCGIATAASY PTV

References

[1]	"RACE cloning and expression of canine cathepsin L." Eto Y., Hirata M., Takeuchi A., Yamamoto Y., Taga A., Taura Y., Takahashi S.Y. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ279008 mRNA. Translation: CAC08809.1.
RefSeq	NP_001003115.1.
UniGene	Cfa.3571
3D structure databases
SMR	Q9GL24. Positions 21-333.
ModBase	Search...
Protein family/group databases
MEROPS	C01.032.
Genome annotation databases
Ensembl	ENSCAFT00000001770; ENSCAFP00000001630; ENSCAFG00000001149; Canis familiaris. [Genome view]
GeneID	403708.
KEGG	cfa:403708.
Organism-specific databases
CTD	403708.
Phylogenomic databases
eggNOG	maNOG14380.
HOVERGEN	Q9GL24.
InParanoid	Q9GL24.
PhylomeDB	Q9GL24.
Enzyme and pathway databases
BRENDA	3.4.22.15. 463.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL1_CANFA

Accession

Primary (citable) accession number: Q9GL24

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)