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Protein

Acrosin

Gene

ACR

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Charge relay system1
Active sitei140Charge relay system1
Active sitei238Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.10. 2668.

Protein family/group databases

MEROPSiS01.223.

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:ACR
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000002753818 – 329AcrosinAdd BLAST312
ChainiPRO_000002753918 – 39Acrosin light chainAdd BLAST22
ChainiPRO_000002754040 – ›329Acrosin heavy chainAdd BLAST›290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...)By similarity1
Disulfide bondi22 ↔ 152Interchain (between light and heavy chains)
Disulfide bondi26 ↔ 160Interchain (between light and heavy chains)
Disulfide bondi71 ↔ 87
Disulfide bondi175 ↔ 244
Disulfide bondi207 ↔ 223
Glycosylationi208N-linked (GlcNAc...)1
Disulfide bondi234 ↔ 264

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ9GL10.

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi54 – 61Combined sources8
Turni62 – 65Combined sources4
Beta strandi66 – 77Combined sources12
Beta strandi80 – 83Combined sources4
Helixi85 – 88Combined sources4
Helixi94 – 96Combined sources3
Beta strandi97 – 102Combined sources6
Beta strandi119 – 128Combined sources10
Turni134 – 137Combined sources4
Beta strandi142 – 148Combined sources7
Beta strandi174 – 180Combined sources7
Beta strandi195 – 202Combined sources8
Helixi204 – 207Combined sources4
Turni210 – 215Combined sources6
Beta strandi221 – 225Combined sources5
Beta strandi230 – 232Combined sources3
Beta strandi241 – 245Combined sources5
Beta strandi247 – 250Combined sources4
Beta strandi252 – 260Combined sources9
Beta strandi262 – 266Combined sources5
Beta strandi271 – 276Combined sources6
Helixi277 – 279Combined sources3
Helixi280 – 287Combined sources8
Helixi289 – 294Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIWX-ray2.10A40-329[»]
L18-39[»]
ProteinModelPortaliQ9GL10.
SMRiQ9GL10.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GL10.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 288Peptidase S1PROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GL10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPTAVLLVL AVSVVARDNT TCDGPCGVRF RQNRQGGVRI IGGQDAAHGA
60 70 80 90 100
WPWMVSLQIF TYHNNRRYHV CGGSLLNSQW LLTAAHCFRI KKKVTDWRLI
110 120 130 140 150
FGAKEVEWGT NKPVKPPLQE RYVEKIIIHE KYSASSEAND IALMKITPPV
160 170 180 190 200
TCGHFIGPGC LPQFRAGPPR VPQTCWVAGW GFLQENARRT SPMLQEARVD
210 220 230 240 250
LIDLGLCNST RWYNGRIRST NVCAGYPEGK IDTCQGDSGG PLMCKDSAEN
260 270 280 290 300
SYVVVGITSW GVGCARAKRP GVYTSTWSYL NWIASKIGST AVHMIQLPTA
310 320
SPASTPGAQA SSGSVQPSVR PPWFFQQIT
Length:329
Mass (Da):36,119
Last modified:March 1, 2001 - v1
Checksum:iB934C569D83E21E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei3291

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278742 mRNA. Translation: CAC15386.1.
UniGeneiOar.871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278742 mRNA. Translation: CAC15386.1.
UniGeneiOar.871.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIWX-ray2.10A40-329[»]
L18-39[»]
ProteinModelPortaliQ9GL10.
SMRiQ9GL10.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.223.

Proteomic databases

PRIDEiQ9GL10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Enzyme and pathway databases

BRENDAi3.4.21.10. 2668.

Miscellaneous databases

EvolutionaryTraceiQ9GL10.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRO_SHEEP
AccessioniPrimary (citable) accession number: Q9GL10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.