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Protein

Acrosin

Gene

ACR

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Charge relay system
Active sitei140 – 1401Charge relay system
Active sitei238 – 2381Charge relay system

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.10. 2668.

Protein family/group databases

MEROPSiS01.223.

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:ACR
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Chaini18 – 329312AcrosinPRO_0000027538Add
BLAST
Chaini18 – 3922Acrosin light chainPRO_0000027539Add
BLAST
Chaini40 – ›329›290Acrosin heavy chainPRO_0000027540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...)By similarity
Disulfide bondi22 ↔ 152Interchain (between light and heavy chains)
Disulfide bondi26 ↔ 160Interchain (between light and heavy chains)
Disulfide bondi71 ↔ 87
Disulfide bondi175 ↔ 244
Disulfide bondi207 ↔ 223
Glycosylationi208 – 2081N-linked (GlcNAc...)
Disulfide bondi234 ↔ 264

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Structurei

Secondary structure

1
329
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi54 – 618Combined sources
Turni62 – 654Combined sources
Beta strandi66 – 7712Combined sources
Beta strandi80 – 834Combined sources
Helixi85 – 884Combined sources
Helixi94 – 963Combined sources
Beta strandi97 – 1026Combined sources
Beta strandi119 – 12810Combined sources
Turni134 – 1374Combined sources
Beta strandi142 – 1487Combined sources
Beta strandi174 – 1807Combined sources
Beta strandi195 – 2028Combined sources
Helixi204 – 2074Combined sources
Turni210 – 2156Combined sources
Beta strandi221 – 2255Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi241 – 2455Combined sources
Beta strandi247 – 2504Combined sources
Beta strandi252 – 2609Combined sources
Beta strandi262 – 2665Combined sources
Beta strandi271 – 2766Combined sources
Helixi277 – 2793Combined sources
Helixi280 – 2878Combined sources
Helixi289 – 2946Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FIWX-ray2.10A40-329[»]
L18-39[»]
ProteinModelPortaliQ9GL10.
SMRiQ9GL10. Positions 40-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GL10.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 288249Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GL10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPTAVLLVL AVSVVARDNT TCDGPCGVRF RQNRQGGVRI IGGQDAAHGA
60 70 80 90 100
WPWMVSLQIF TYHNNRRYHV CGGSLLNSQW LLTAAHCFRI KKKVTDWRLI
110 120 130 140 150
FGAKEVEWGT NKPVKPPLQE RYVEKIIIHE KYSASSEAND IALMKITPPV
160 170 180 190 200
TCGHFIGPGC LPQFRAGPPR VPQTCWVAGW GFLQENARRT SPMLQEARVD
210 220 230 240 250
LIDLGLCNST RWYNGRIRST NVCAGYPEGK IDTCQGDSGG PLMCKDSAEN
260 270 280 290 300
SYVVVGITSW GVGCARAKRP GVYTSTWSYL NWIASKIGST AVHMIQLPTA
310 320
SPASTPGAQA SSGSVQPSVR PPWFFQQIT
Length:329
Mass (Da):36,119
Last modified:March 1, 2001 - v1
Checksum:iB934C569D83E21E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei329 – 3291

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278742 mRNA. Translation: CAC15386.1.
UniGeneiOar.871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278742 mRNA. Translation: CAC15386.1.
UniGeneiOar.871.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FIWX-ray2.10A40-329[»]
L18-39[»]
ProteinModelPortaliQ9GL10.
SMRiQ9GL10. Positions 40-299.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Enzyme and pathway databases

BRENDAi3.4.21.10. 2668.

Miscellaneous databases

EvolutionaryTraceiQ9GL10.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRO_SHEEP
AccessioniPrimary (citable) accession number: Q9GL10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.