Reviewed, UniProtKB/Swiss-Prot Q9GL01 (RPN2_PIG)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
    Ribophorin-2
    Ribophorin II
      Short name=RPN-II

Gene names

Name:

RPN2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SWP1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
PTM	Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell oligosaccharyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 629	607	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2		PRO_5000066372
Regions
	Topological domain	23 – 541	519	Lumenal Potential
	Transmembrane	542 – 562	21	Potential
	Transmembrane	571 – 591	21	Potential
	Transmembrane	595 – 615	21	Potential
	Topological domain	616 – 629	14	Cytoplasmic Potential
	Compositional bias	257 – 262	6	Poly-Ala
	Compositional bias	270 – 275	6	Poly-Val
	Compositional bias	554 – 557	4	Poly-Leu
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Cross-link	154		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GL01-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 93F22E2D0426C98C	FASTA	629	69,274
	10 20 30 40 50 60 MAPPGSRTVL LLALTIIART QALKPTHYLT KHDVERLKAS LDRPFTSLES AFYSIVGLSS 70 80 90 100 110 120 LGAQVPDEKK ACTFIKSNLD PSNVDSLFYP PQSSQALSGC EISISNETKD LLLAAVSEDS 130 140 150 160 170 180 SVTQIYHAVA ALSGFGLPLA SQEALGALTA RLSKEETVLA TVQALQTASY LSQQADLRSI 190 200 210 220 230 240 VEEIEDLVAR LDELGGVYLQ FEEGLLETTA LFVAATYKLM DHEGTEPSIK EDQVIQLMNT 250 260 270 280 290 300 IFSKKNFESL PEAFSVASAA AALSQNRYHV PVVVVPEGSP SDTQEQAFLR LQVTNVLSQP 310 320 330 340 350 360 LTQATVKLEH AKSVASRATV LQKTSFTPVG DVFELNFVNV KFSSGYYDFS VKVEGDNRYI 370 380 390 400 410 420 ANTVELRVKI STEVGITNVD LSTVDKDQSI APKTTRVTYP AKAKGPFIAD SPQNFALFFQ 430 440 450 460 470 480 LVDVNTGAEL TPHQTFVRLH NQKTGQEVVF VAEPDSKNVY RFELDTSERK IEFDSASGTY 490 500 510 520 530 540 TLYLIIGDAT LKNPIHWNVA DVVIRFPEED APSTVLSKNL FTAKQEIQHL FRDPEKRPPT 550 560 570 580 590 600 VVSNTFTGLI LSPLLLLFAL WIRIGAKISN FTFGLTIIFH LGHAMLAMYV YWTQLNMFQT 610 620 LKYLAILGSV TFLAGNRMLA QQAIKRTAH

References

[1]	"The oligosaccharyltransferase complex from pig liver: cDNA cloning, expression and functional characterisation." Hardt B., Aparicio R., Bause E. Glycoconj. J. 17:767-779(2000) [PubMed: 11443278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ293583 mRNA. Translation: CAC10571.1.
RefSeq	NP_999106.1.
UniGene	Ssc.11153
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	396981.
KEGG	ssc:396981.
Organism-specific databases
CTD	396981.
Phylogenomic databases
HOVERGEN	Q9GL01.
Enzyme and pathway databases
BRENDA	2.4.1.119. 249.
Family and domain databases
InterPro	IPR008814. Ribophorin_II. [Graphical view]
PANTHER	PTHR12640. Ribophorin_II. 1 hit.
Pfam	PF05817. Ribophorin_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPN2_PIG

Accession

Primary (citable) accession number: Q9GL01

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2001
Last modified:	October 13, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)