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Protein

Junctophilin-2

Gene

JPH2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca2+ signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions (By similarity).By similarity

GO - Molecular functioni

Names & Taxonomyi

Protein namesi
Recommended name:
Junctophilin-2
Short name:
JP-2
Alternative name(s):
Junctophilin type 2
Gene namesi
Name:JPH2
Synonyms:JP2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 672CytoplasmicSequence analysisAdd BLAST672
Transmembranei673 – 693Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598491 – 694Junctophilin-2Add BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1
Modified residuei445PhosphoserineBy similarity1
Modified residuei447PhosphoserineBy similarity1
Modified residuei466PhosphoserineBy similarity1
Modified residuei474PhosphothreonineBy similarity1
Modified residuei483PhosphoserineBy similarity1
Modified residuei487PhosphothreonineBy similarity1
Modified residuei536PhosphoserineBy similarity1
Modified residuei542PhosphoserineBy similarity1
Modified residuei596PhosphoserineBy similarity1
Modified residuei600PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000019570.

Structurei

3D structure databases

ProteinModelPortaliQ9GKY7.
SMRiQ9GKY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 36MORN 1Add BLAST23
Repeati38 – 59MORN 2Add BLAST22
Repeati60 – 79MORN 3Add BLAST20
Repeati82 – 104MORN 4Add BLAST23
Repeati106 – 128MORN 5Add BLAST23
Repeati129 – 151MORN 6Add BLAST23
Repeati290 – 312MORN 7Add BLAST23
Repeati313 – 335MORN 8Add BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 142Gly-richAdd BLAST140
Compositional biasi372 – 407Ala-richAdd BLAST36

Domaini

The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, by interacting with phospholipids. Has affinity for phosphatidylserine, and phosphorylated phosphatidylinositols including PtdIns3P, PtdIns4P, PtdIns5P, PtdIns(3,5)P2 and PtdIns(3,4,5)P3 (By similarity).By similarity

Sequence similaritiesi

Belongs to the junctophilin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0231. Eukaryota.
COG4642. LUCA.
HOGENOMiHOG000264244.
HOVERGENiHBG031648.
InParanoidiQ9GKY7.
KOiK19530.

Family and domain databases

InterProiView protein in InterPro
IPR017191. Junctophilin.
IPR003409. MORN.
PfamiView protein in Pfam
PF02493. MORN. 8 hits.
PIRSFiPIRSF037387. Junctophilin. 1 hit.
SMARTiView protein in SMART
SM00698. MORN. 6 hits.

Sequencei

Sequence statusi: Complete.

Q9GKY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGRFDFDD GGAYCGGWEG GKAHGHGLCT GPKGQGEYSG SWNFGFEVAG
60 70 80 90 100
VYTWPSGNTF EGYWSQGKRH GLGIETKGRW LYKGEWTHGF KGRYGTRQST
110 120 130 140 150
SSGAKYEGTW NNGLQDGYGT ETYADGGTYQ GQFTNGMRHG YGVRQSVPYG
160 170 180 190 200
MAVVVRSPLR TSLSSLRSEH SNGTVAPDSP ASPAADGPAL PSPAIPRGGF
210 220 230 240 250
ALSLLANAEA ARAPKGGGLF PRGALLGKLR RAESRTSVGS QRSRVSFLKS
260 270 280 290 300
DLSSGASDAA STASLGEGAE GADDAAPFEA DIDATTTETY MGEWKNDKRS
310 320 330 340 350
GFGVSERSSG LRYEGEWLDN LRHGYGCTTL PDGHREEGKY RHNVLVKGTK
360 370 380 390 400
RRVLPLKSNK VRQKVEHSVE GAQRAAAIAR QKAEIAVSRT SHARAKAEAA
410 420 430 440 450
EQAALAANQE SNIARSLARE LAPDFYQPGP EYQKRRLLQE ILEHSESLLE
460 470 480 490 500
PPDRGAAGLP QPPRESPQLH ERETPRPEGG PPSPAGTPPQ PKRPRPGASK
510 520 530 540 550
DGLLGPGAWN GEPSGGSGGE GSRPATPAAA GAGRRSPARP ASEHMAIEAL
560 570 580 590 600
QAPPAPSREP EVALYRGYHS YAVRTAPPAP PPFEDDPQPE AADPDSAPAS
610 620 630 640 650
PATAPGQAPA LGNPEPAPES PAKLEPKPIV PKAKARKTEA RGLSKTGAKK
660 670 680 690
KPRKEAAQAA EAEVEVEEVP NTVLICMVIL LNIGLAILFV HLLT
Length:694
Mass (Da):73,343
Last modified:March 1, 2001 - v1
Checksum:iB0C3811BC2540016
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024444 mRNA. Translation: BAB20318.1.
RefSeqiNP_001075467.1. NM_001081998.1.
UniGeneiOcu.2521.

Genome annotation databases

GeneIDi100008613.
KEGGiocu:100008613.

Similar proteinsi

Entry informationi

Entry nameiJPH2_RABIT
AccessioniPrimary (citable) accession number: Q9GKY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: March 1, 2001
Last modified: July 5, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families