Q9GKM4 (BCAT1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Branched-chain-amino-acid aminotransferase, cytosolic
Short name=BCAT(c)
EC=2.6.1.42

Gene names

Name:

BCAT1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Catalytic activity	L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate. L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate. L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in muscles. Ref.1
Sequence similarities	Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological_process	branched-chain amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-isoleucine transaminase activity Inferred from electronic annotation. Source: EC L-leucine transaminase activity Inferred from electronic annotation. Source: EC L-valine transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Branched-chain-amino-acid aminotransferase, cytosolic		PRO_0000103295
Amino acid modifications
	Modified residue	221	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GKM4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BF245858BBE684D2	FASTA	385	43,073
	10 20 30 40 50 60 MDCNNGCSAE GTGEGGSKEP VETFKAEDLI ITRATILKEK PDPSTLVFGT VFTDHMLTVE 70 80 90 100 110 120 WSLELGWEKP RIKPLQNLSL HPGSSALHYA VELFEGLKAF RGVDNKIRLF RPNLNMDRMY 130 140 150 160 170 180 RSAMRATLPA FDKKELLECI QQLVKLDEEW VPYSTSASLY IRPTFIGTEP SLGVKKPTKA 190 200 210 220 230 240 LLFVILSPVG PYFSSGSFNP VSLWANPKYV RAWKGGTGDC KMGGNYGSSL FAQCEAVENA 250 260 270 280 290 300 CQQVLWLYGE ENQITEVGTM NLFLYWINED GEEELATPPL DGIILPGVMR QSILDLAHKW 310 320 330 340 350 360 GEFKVSERYL TMDDLTTAVE ENRVREMFGS GTACVVCPVS TILYKDETIH IPTMENGPKL 370 380 ASRILEKLTD IQYGREESDW TITVA

References

[1]

"Sheep cytosolic branched-chain amino acid aminotransferase: cDNA cloning, primary structure and molecular modelling and its unique expression in muscles." Bonfils J., Faure M., Gibrat J.-F., Glomot F., Papet I. Biochim. Biophys. Acta 1494:129-136(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, 3D-STRUCTURE MODELING. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184916 mRNA. Translation: AAG16994.1.
RefSeq	NP_001009444.1. NM_001009444.1.
UniGene	Oar.797.
3D structure databases
ProteinModelPortal	Q9GKM4.
SMR	Q9GKM4. Positions 23-384.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443485.
Organism-specific databases
CTD	586.
Phylogenomic databases
HOVERGEN	HBG050678.
Enzyme and pathway databases
BRENDA	2.6.1.42. 4472.
Family and domain databases
InterPro	IPR001544. Aminotrans_IV. IPR018300. Aminotrans_IV_CS. IPR005786. B_amino_transII. [Graphical view]
PANTHER	PTHR11825. PTHR11825. 1 hit.
Pfam	PF01063. Aminotran_4. 1 hit. [Graphical view]
PIRSF	PIRSF006468. BCAT1. 1 hit.
SUPFAM	SSF56752. Aminotrans_IV. 1 hit.
TIGRFAMs	TIGR01123. ilvE_II. 1 hit.
PROSITE	PS00770. AA_TRANSFER_CLASS_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCAT1_SHEEP

Accession

Primary (citable) accession number: Q9GKM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program