Reviewed, UniProtKB/Swiss-Prot Q9GKL8 (CATL1_CERAE)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Alternative name(s):
    Major excreted protein
      Short name=MEP
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL1
Synonyms:	CTSL

Organism

Cercopithecus aethiops (Green monkey) (Grivet)

Taxonomic identifier

9534 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Chlorocebus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes By similarity.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds By similarity.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 113	96	Activation peptide By similarity		PRO_0000287866
	Chain	114 – 288	175	Cathepsin L1 heavy chain		PRO_0000287867
	Propeptide	289 – 291	3	By similarity		PRO_0000287868
	Chain	292 – 333	42	Cathepsin L1 light chain		PRO_0000287869
Sites
	Active site	138	1	By similarity
	Active site	276	1	By similarity
	Active site	300	1	By similarity
Amino acid modifications
	Glycosylation	221	1	N-linked (GlcNAc...)
	Disulfide bond	135 ↔ 178		By similarity
	Disulfide bond	169 ↔ 211		By similarity
	Disulfide bond	269 ↔ 322		Interchain (between heavy and light chains) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GKL8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F6CB37EF18A36E59	FASTA	333	37,430
	10 20 30 40 50 60 MNPTFILAAL CLGIASATLT FNHSLEAQWT KWKAMHNRLY GMNEEGWRRA VWEKNMKMIE 70 80 90 100 110 120 LHNQEYSQGK HSFTMAMNTF GDMTSEEFRQ VMNGFQNRKP RKGKVFQEPL FYEAPRSVDW 130 140 150 160 170 180 REKGYVTPVK NQGQCGSCWA FSATGALEGQ MFRKTGKLVS LSEQNLVDCS GPQGNEGCNG 190 200 210 220 230 240 GLMDYAFQYV ADNGGLDSEE SYPYEATEES CKYNPEYSVA NDTGFVDIPK QEKALMKAVA 250 260 270 280 290 300 TVGPISVAID AGHESFMFYK EGIYFEPDCS SEDMDHGVLV VGYGFESTES DNSKYWLVKN 310 320 330 SWGEEWGMGG YIKMAKDRRN HCGIASAASY PTV

References

[1]	"Molecular cloning of a cDNA encoding a nuclear localizing cathepsin L-like cysteine protease, SPase." Nishinaka T., Huynh T., Cheng C.-H., Chiu R. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF201700 mRNA. Translation: AAG35605.1.
3D structure databases
HSSP	HSSP built from PDB template 1ICF based on UniProtKB P07711.
SMR	Q9GKL8. Positions 18-333.
ModBase	Search...
Protein family/group databases
MEROPS	C01.032. I29.001.
Phylogenomic databases
HOVERGEN	Q9GKL8.
Enzyme and pathway databases
BRENDA	3.4.22.15. 2052.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL1_CERAE

Accession

Primary (citable) accession number: Q9GKL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)