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Protein

Steroidogenic factor 1

Gene

NR5A1

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei341 – 3411Lipid headgroup; via amide nitrogenBy similarity
Binding sitei436 – 4361Lipid headgroupBy similarity
Binding sitei440 – 4401Lipid headgroupBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi10 – 8576Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Steroidogenic factor 1
Short name:
SF-1
Short name:
STF-1
Alternative name(s):
Nuclear receptor subfamily 5 group A member 1
Gene namesi
Name:NR5A1
Synonyms:SF1
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Steroidogenic factor 1PRO_0000053730Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki119 – 119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki194 – 194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei203 – 2031Phosphoserine; by CDK7By similarity

Post-translational modificationi

Acetylation stimulates the transcriptional activity.By similarity
Sumoylation reduces CDK7-mediated phosophorylation on Ser-203.By similarity
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9GKL2.
PRIDEiQ9GKL2.

Interactioni

Subunit structurei

Binds DNA as a monomer (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NR0B2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000011635.

Structurei

3D structure databases

ProteinModelPortaliQ9GKL2.
SMRiQ9GKL2. Positions 10-111, 236-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni260 – 34788Ligand-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 3321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri49 – 7325NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
HOGENOMiHOG000063718.
HOVERGENiHBG106677.
InParanoidiQ9GKL2.
KOiK08560.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GKL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT
60 70 80 90 100
ESQSCKIDKT LRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK
110 120 130 140 150
RDRALKQQKK AQIRANGFKL ETGPPMGVPP PPPPPPDYML PPGLHVPEPK
160 170 180 190 200
GLASGPPAGP LGDFGAPALP MAVPSTNGPL AGYLYPAFPG RAIKSEYPEP
210 220 230 240 250
YASPPQPGPP YGYPEPFSGG PGVPELILQL LQLEPDEDQV RARIIGCLQE
260 270 280 290 300
PAKGRPDQPA SFNLLCRMAD QTFISIVDWA RRCMVFKELE VADQMTLLQN
310 320 330 340 350
CWSELLVFDH IYRQVQHGKE GSTLLVTGQE VELTTVAAQA GSLLHGLVLR
360 370 380 390 400
AQELVLQMHA LQLDRQEFVC LKFLILFSLD VKFLNNHSLV KDAQEKANTA
410 420 430 440 450
LLDYTLCHYP HCGDKFQQLL LCLVEVRALS MQAKEYLYHK HLGNEMPRNN
460
LLIEMLQAKQ T
Length:461
Mass (Da):51,644
Last modified:March 1, 2001 - v1
Checksum:i85909263A89A1A9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203911 mRNA. Translation: AAG35648.1.
RefSeqiNP_001075320.1. NM_001081851.1.
UniGeneiEca.12998.

Genome annotation databases

GeneIDi100033902.
KEGGiecb:100033902.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203911 mRNA. Translation: AAG35648.1.
RefSeqiNP_001075320.1. NM_001081851.1.
UniGeneiEca.12998.

3D structure databases

ProteinModelPortaliQ9GKL2.
SMRiQ9GKL2. Positions 10-111, 236-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000011635.

Proteomic databases

PaxDbiQ9GKL2.
PRIDEiQ9GKL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100033902.
KEGGiecb:100033902.

Organism-specific databases

CTDi2516.

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
HOGENOMiHOG000063718.
HOVERGENiHBG106677.
InParanoidiQ9GKL2.
KOiK08560.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression and regulation of transcripts encoding two members of the NR5A nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process."
    Boerboom D., Pilon N., Behdjani R., Silversides D.W., Sirois J.
    Endocrinology 141:4647-4656(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Corpus luteum and Ovarian follicle.

Entry informationi

Entry nameiSTF1_HORSE
AccessioniPrimary (citable) accession number: Q9GKL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.