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Protein

Beta-casein

Gene

CSN2

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important role in determination of the surface properties of the casein micelles.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-casein
Gene namesi
Name:CSN2By similarity
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysis1 PublicationAdd
BLAST
Chaini16 – 241226Beta-caseinPRO_0000004474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Phosphoserine; in form 4-P, form 5-P, form 6-P and form 7-P1 Publication
Modified residuei25 – 251Phosphoserine; in form 7-P1 Publication
Modified residuei27 – 271Phosphothreonine; in form 6-P and form 7-P1 Publication
Modified residuei30 – 301PhosphoserineBy similarity
Modified residuei32 – 321PhosphoserineBy similarity
Modified residuei33 – 331Phosphoserine; in form 5-P, form 6-P and form 7-P1 Publication
Modified residuei38 – 381Phosphoserine; in form 4-P, form 5-P, form 6-P and form 7-P1 Publication
Modified residuei39 – 391Phosphoserine; in form 4-P, form 5-P, form 6-P and form 7-P1 Publication
Modified residuei40 – 401Phosphoserine; in form 4-P, form 5-P, form 6-P and form 7-P1 Publication
Modified residuei150 – 1501Deamidated asparagine1 Publication

Post-translational modificationi

There are at least five different forms found in milk, with varying degrees of phosphorylation. These include form 3-P which is phosphorylated at three sites that have not been determined, this form is present in very low amounts, form 4-P which is phosphorylated at four sites, form 5-P which is phosphorylated at five sites, form 6-P which is phosphorylated at six sites, and form 7-P which is phosphorylated at seven sites.2 Publications
Spontaneous deamidation of Asn-150 produces aspartate or isoaspartate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei30 – 301Not phosphorylated
Sitei32 – 321Not phosphorylated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9GKK3.
PeptideAtlasiQ9GKK3.

PTM databases

iPTMnetiQ9GKK3.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.Curated

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007906.

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-casein family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYJE. Eukaryota.
ENOG411197Z. LUCA.
GeneTreeiENSGT00390000001890.
HOGENOMiHOG000074044.
HOVERGENiHBG004973.
InParanoidiQ9GKK3.
KOiK17107.
OMAiEIMEVPK.
OrthoDBiEOG7WQ7VM.
TreeFamiTF336929.

Family and domain databases

InterProiIPR001588. Casein.
IPR016345. Casein_beta.
IPR031305. Casein_CS.
[Graphical view]
PANTHERiPTHR11500. PTHR11500. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PIRSFiPIRSF002372. Beta-casein. 1 hit.
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9GKK3-1) [UniParc]FASTAAdd to basket

Also known as: B1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILILACLV ALALAREKEE LNVSSETVES LSSNEPDSSS EESITHINKE
60 70 80 90 100
KLQKFKHEGQ QQREVERQDK ISRFVQPQPV VYPYAEPVPY AVVPQSILPL
110 120 130 140 150
AQPPILPFLQ PEIMEVSQAK ETILPKRKVM PFLKSPIVPF SERQILNPTN
160 170 180 190 200
GENLRLPVHL IQPFMHQVPQ SLLQTLMLPS QPVLSPPQSK VAPFPQPVVP
210 220 230 240
YPQRDTPVQA FLLYQDPRLG PTGELDPATQ PIVAVHNPVI V
Length:241
Mass (Da):27,049
Last modified:March 7, 2006 - v3
Checksum:i140B6EBF3D2323C0
GO
Isoform 21 Publication (identifier: Q9GKK3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-49: Missing.

Show »
Length:233
Mass (Da):26,126
Checksum:i492A07BD010FBF88
GO
Isoform 31 Publication (identifier: Q9GKK3-3) [UniParc]FASTAAdd to basket

Also known as: low molecular weight beta-casein1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     65-196: Missing.

Note: Accounts for 4% of total casein.1 Publication
Show »
Length:109
Mass (Da):12,127
Checksum:i40C3C1961A496762
GO

Mass spectrometryi

Isoform 1 : Molecular mass is 25514±3 Da from positions 16 - 241. Determined by ESI. Dephosphorylated.1 Publication
Isoform 3 : Molecular mass is 10591±2 Da from positions 16 - 241. Determined by ESI. Dephosphorylated.1 Publication
Isoform 3 : Molecular mass is 10911 Da from positions 16 - 241. Determined by ESI. Form 4-P.1 Publication
Isoform 3 : Molecular mass is 10991 Da from positions 16 - 241. Determined by ESI. Form 5-P.1 Publication
Isoform 3 : Molecular mass is 11071 Da from positions 16 - 241. Determined by ESI. Form 6-P.1 Publication
Isoform 3 : Molecular mass is 11150 Da from positions 16 - 241. Determined by ESI. Form 7-P.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 498Missing in isoform 2. 1 PublicationVSP_051779
Alternative sequencei65 – 196132Missing in isoform 3. 1 PublicationVSP_051780Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214526 mRNA. Translation: AAG43954.1.
RefSeqiNP_001075321.1. NM_001081852.1. [Q9GKK3-2]
UniGeneiEca.12894.

Genome annotation databases

EnsembliENSECAT00000010264; ENSECAP00000007906; ENSECAG00000009837. [Q9GKK3-1]
GeneIDi100033903.
KEGGiecb:100033903.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214526 mRNA. Translation: AAG43954.1.
RefSeqiNP_001075321.1. NM_001081852.1. [Q9GKK3-2]
UniGeneiEca.12894.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007906.

PTM databases

iPTMnetiQ9GKK3.

Proteomic databases

PaxDbiQ9GKK3.
PeptideAtlasiQ9GKK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSECAT00000010264; ENSECAP00000007906; ENSECAG00000009837. [Q9GKK3-1]
GeneIDi100033903.
KEGGiecb:100033903.

Organism-specific databases

CTDi1447.

Phylogenomic databases

eggNOGiENOG410IYJE. Eukaryota.
ENOG411197Z. LUCA.
GeneTreeiENSGT00390000001890.
HOGENOMiHOG000074044.
HOVERGENiHBG004973.
InParanoidiQ9GKK3.
KOiK17107.
OMAiEIMEVPK.
OrthoDBiEOG7WQ7VM.
TreeFamiTF336929.

Family and domain databases

InterProiIPR001588. Casein.
IPR016345. Casein_beta.
IPR031305. Casein_CS.
[Graphical view]
PANTHERiPTHR11500. PTHR11500. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PIRSFiPIRSF002372. Beta-casein. 1 hit.
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of equine cDNA sequences for alphaS1-, beta- and kappa-casein."
    Lenasi T., Rogelj I., Dovc P.
    J. Dairy Res. 70:29-36(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Warmblood1 Publication.
    Tissue: Lactating mammary glandImported.
  2. "Determination of the phosphorylation level and deamidation susceptibility of equine beta-casein."
    Girardet J.-M., Miclo L., Florent S., Molle D., Gaillard J.-L.
    Proteomics 6:3707-3717(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-241 (ISOFORM 1), DEAMIDATION AT ASN-150, MASS SPECTROMETRY.
    Strain: Haflinger1 Publication.
    Tissue: Milk1 Publication.
  3. "The primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk."
    Miclo L., Girardet J.-M., Egito A.S., Molle D., Martin P., Gaillard J.-L.
    Proteomics 7:1327-1335(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-241 (ISOFORM 3), MASS SPECTROMETRY.
    Strain: Haflinger1 Publication.
    Tissue: Milk1 Publication.
  4. "Proteomic tools to characterize the protein fraction of Equidae milk."
    Miranda G., Mahe M.-F., Leroux C., Martin P.
    Proteomics 4:2496-2509(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-50 (ISOFORM 1), PROTEIN SEQUENCE OF 16-58 (ISOFORM 2).
    Strain: Welsh pony1 Publication.
    Tissue: Milk1 Publication.
  5. "Separation and characterization of mares' milk alpha(s1)-, beta-, kappa-caseins, gamma-casein-like, and proteose peptone component 5-like peptides."
    Egito A.S., Miclo L., Lopez C., Adam A., Girardet J.-M., Gaillard J.-L.
    J. Dairy Sci. 85:697-706(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-30, SUBCELLULAR LOCATION.
    Strain: Haflinger1 Publication.
    Tissue: Milk1 Publication.
  6. "Identification of phosphorylation sites of equine beta-casein isoforms."
    Mateos A., Girardet J.M., Molle D., Corbier C., Gaillard J.L., Miclo L.
    Rapid Commun. Mass Spectrom. 24:1533-1542(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-24; SER-25; THR-27; SER-33; SER-38; SER-39 AND SER-40.

Entry informationi

Entry nameiCASB_HORSE
AccessioniPrimary (citable) accession number: Q9GKK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 7, 2006
Last modified: July 6, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.