Reviewed, UniProtKB/Swiss-Prot Q9GJX5 (CP4AL_PIG)

Names and origin

Protein names

Recommended name:
    Taurochenodeoxycholic 6 alpha-hydroxylase
    EC=1.14.13.97
Alternative name(s):
    Cytochrome P450 4A21
    CYPIVA21

Gene names

Name:

CYP4A21

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 6 alpha hydroxylation oxidation of taurodeoxycholate to produce the pig specific bile acid taurohyocholic acid. Ref.1 Ref.3 Ref.4
Catalytic activity	Taurochenodeoxycholate + NADPH + O2 = taurohyocholate + NADP+ + H2O. Lithocholate + NADPH + O2 = hyodeoxycholate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Primarily expressed in liver. Low expression in kidney. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro taurochenodeoxycholate 6alpha-hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 504	503	Taurochenodeoxycholic 6 alpha-hydroxylase		PRO_0000280742
Regions
	Transmembrane	6 – 26	21	Potential
	Transmembrane	110 – 130	21	Potential
Sites
	Metal binding	451	1	Iron (heme axial ligand) By similarity
Experimental info
	Mutagenesis	314 – 319	6	AAGHDS → FEGHDT: Complete loss of activity. Ref.1
	Sequence conflict	135	1	Q → P in CAE52532. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9GJX5-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 101DAF0494F1BF7D	FASTA	504	57,216
	10 20 30 40 50 60 MTVPALASVS GLLQVASLLG LLLLLLKAAQ LYLRRQWLLK ALQQFPSPPS HWLYGHSREF 70 80 90 100 110 120 QEESELQPLL KRVEKYPSAC ARWLWGTRAM VLVYDPDYMK VVLARSEPKA PVLYRLLIPW 130 140 150 160 170 180 IGCGLLLLNG QMWFQRRRML TPAFHYDILK PYVGLMAKSV QVMLDKWEQL VAQDPRLEIV 190 200 210 220 230 240 GPVSLMTLDT IMKCAFSHQG SAQTDGDSQS YIQAIWDLKN LIFSRLRSAF LQNDIIYRLS 250 260 270 280 290 300 PEGRQCQRAC QKVHQHTDRV IQLRKTHLQK EGEMENVKKK RHLDFLDILL FARMENGNSL 310 320 330 340 350 360 SDTDVRAEVD TFMAAGHDST ASGISWVLYA LASNPEHQQR CREEIQGLLG DGTSITWDHL 370 380 390 400 410 420 DQMPYTTMCI KEALRLYPPV PSVGRELSKP ITFPDGRSLP AGIILSLSIY GLHHNPQVWP 430 440 450 460 470 480 NPEEFDPSRF APGSARHSHA FMPFSGGSRN CIGKQFAMNE MKVVVALTLL RFELAPDPSR 490 500 IPVPIQGIVL KSKNGIHLNL RKIP

References

[1]	"Cloning and expression of a pig liver taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily." Lundell K., Hansson R., Wikvall K. J. Biol. Chem. 276:9606-9612(2001) [PubMed: 11113117] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF 314-ALA--SER-319. Tissue: Liver.
[2]	"The porcine taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21) gene: evolution by gene duplication and gene conversion." Lundell K. Biochem. J. 378:1053-1058(2004) [PubMed: 14641109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42 AND 83-504.
[3]	"Characterisation of taurochenodeoxycholic acid 6 alpha-hydroxylase from pig liver microsomes." Araya Z., Hellman U., Hansson R. Eur. J. Biochem. 231:855-861(1995) [PubMed: 7649186] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21; 59-71; 83-88; 228-238; 430-449 AND 472-480, FUNCTION.
[4]	"Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver." Bostroem H. J. Lipid Res. 27:807-812(1986) [PubMed: 3095476] [Abstract] Cited for: FUNCTION. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278474 mRNA. Translation: CAC19358.1. AJ586859 Genomic DNA. Translation: CAE52546.1. AJ586618 Genomic DNA. Translation: CAE52532.1.
RefSeq	NP_999590.1.
UniGene	Ssc.15895
3D structure databases
HSSP	HSSP built from PDB template 1SMI based on UniProtKB P14779.
SMR	Q9GJX5. Positions 42-504.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000004307; ENSSSCP00000004207; ENSSSCG00000003893; Sus scrofa. [Genome view]
GeneID	403327.
KEGG	ssc:403327.
Organism-specific databases
CTD	403327.
Phylogenomic databases
HOVERGEN	Q9GJX5.
Enzyme and pathway databases
BRENDA	1.14.13.97. 249.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP4AL_PIG

Accession

Primary (citable) accession number: Q9GJX5
Secondary accession number(s): Q70BW2, Q70BZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)