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Protein

Sodium-dependent dopamine transporter

Gene

SLC6A3

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (By similarity).By similarity

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi77Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi78Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi82Sodium 2By similarity1
Sitei105Contributes to high-affinity binding to cocaineBy similarity1
Metal bindingi321Sodium 2By similarity1
Metal bindingi353Sodium 2By similarity1
Metal bindingi418Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi421Sodium 1By similarity1
Metal bindingi422Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Symport, Transport
LigandMetal-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter
Short name:
DA transporter
Short name:
DAT
Alternative name(s):
Solute carrier family 6 member 3
Gene namesi
Name:SLC6A3
Synonyms:DAT1
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68CytoplasmicSequence analysisAdd BLAST68
Transmembranei69 – 89Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei96 – 116Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei140 – 160Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini161 – 237ExtracellularSequence analysisAdd BLAST77
Transmembranei238 – 256Helical; Name=4Sequence analysisAdd BLAST19
Transmembranei265 – 282Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei318 – 335Helical; Name=6Sequence analysisAdd BLAST18
Transmembranei347 – 368Helical; Name=7Sequence analysisAdd BLAST22
Transmembranei401 – 420Helical; Name=8Sequence analysisAdd BLAST20
Transmembranei447 – 465Helical; Name=9Sequence analysisAdd BLAST19
Transmembranei481 – 501Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei522 – 541Helical; Name=11Sequence analysisAdd BLAST20
Transmembranei560 – 578Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini579 – 620CytoplasmicSequence analysisAdd BLAST42

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147521 – 620Sodium-dependent dopamine transporterAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi180 ↔ 189By similarity
Glycosylationi181N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi306InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity).By similarity

Chemistry databases

BindingDBiQ9GJT6.

Structurei

3D structure databases

ProteinModelPortaliQ9GJT6.
SMRiQ9GJT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni561 – 590Interaction with TGFB1I1By similarityAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG071421.
KOiK05036.

Family and domain databases

InterProiView protein in InterPro
IPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiView protein in Pfam
PF00209. SNF. 1 hit.
PRINTSiPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiView protein in PROSITE
PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9GJT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSKCSVGL MSSVVAPAKE PNAMGPKEVE LILVKEQNGV QLTSSTLTNP
60 70 80 90 100
RQSPVEAQDR ETWGKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV
110 120 130 140 150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISL
160 170 180 190 200
YVGFFYNVII AWALHYLFSS FTTELPWIHC NNSWNSPNCS DAHSGDSGGN
210 220 230 240 250
GPGLNDTFGT TPAAEYFERG VLHLHQSHGI DDLGPPRWQL TACLVLVIVL
260 270 280 290 300
LYFSLWKGVK TSGKVVWITA TMPYVVLTAL LLRGVTLPGA IDGIRAYLSV
310 320 330 340 350
DFYRLCEASV WIDAATQVCF SLGVGFGVLI AFSSYNKFTN NCYRDAIVTT
360 370 380 390 400
SINSLTSFSS GFVVFSFLGY MAQKHSVPIG DVAKDGPGLI FIIYPEAIAT
410 420 430 440 450
LPLSSAWAVV FFIMLLTLGI DSAMGGMESV ITGLIDEFQL LHRHRELFTL
460 470 480 490 500
FIVLATFLLS LFCVTNGGIY VFTLLDHFAA GTSILFGVLI EAIGVAWFYG
510 520 530 540 550
VGQFSDDIQQ MTGQRPSLYW RLCWKLVSPC FLLFVVVVSI VTFRPPHYGA
560 570 580 590 600
YIFPDWANAL GWVIATSSMA MVPIYAAYKF CSLPGSFREK LAYAIAPEKD
610 620
RELVDRGEVR QFTLRHWLKV
Length:620
Mass (Da):68,452
Last modified:March 1, 2001 - v1
Checksum:i5A9B9379DFEDF0FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286447 mRNA. Translation: AAG00538.1.
RefSeqiNP_001270253.1. NM_001283324.1.
UniGeneiMfa.5823.

Genome annotation databases

GeneIDi102142756.
KEGGimcf:102142756.

Similar proteinsi

Entry informationi

Entry nameiSC6A3_MACFA
AccessioniPrimary (citable) accession number: Q9GJT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2001
Last modified: May 10, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families