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Protein

Glycine receptor subunit beta

Gene

GLRB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei307 – 3071Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit beta
Alternative name(s):
Glycine receptor 58 kDa subunit
Gene namesi
Name:GLRB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cell junctionsynapse By similarity
  • Cell projectiondendrite By similarity
  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasm By similarity

  • Note: Retained in the cytoplasm upon heterologous expression by itself. Coexpression with GPHN promotes expression at the cell membrane. Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 268246ExtracellularBy similarityAdd
BLAST
Transmembranei269 – 29022Helical; Name=1By similarityAdd
BLAST
Topological domaini291 – 2955CytoplasmicBy similarity
Transmembranei296 – 31621Helical; Name=2By similarityAdd
BLAST
Topological domaini317 – 32711ExtracellularBy similarityAdd
BLAST
Transmembranei328 – 34821Helical; Name=3By similarityAdd
BLAST
Topological domaini349 – 475127CytoplasmicBy similarityAdd
BLAST
Transmembranei476 – 49621Helical; Name=4By similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 497475Glycine receptor subunit betaPRO_0000236240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi183 ↔ 197By similarity
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence analysis
Disulfide bondi243 ↔ 255By similarity
Modified residuei391 – 3911PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9GJS9.
PRIDEiQ9GJS9.

Interactioni

Subunit structurei

Heteropentamer composed of GLRB and GLRA1. Heteropentamer composed of GLRB and GLRA2. Heteropentamer composed of GLRB and GLRA3. Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits. Interacts with GPHN.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029010.

Structurei

3D structure databases

ProteinModelPortaliQ9GJS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 2537Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3644. Eukaryota.
ENOG410XP43. LUCA.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ9GJS9.
KOiK05196.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR008060. Glycine_rcpt_B.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF29. PTHR18945:SF29. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01677. GLYRBETA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GJS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLAVAFF ILISLCIEEA YSKEKSSKKG KGKKKQYLCP SQQSAEDLAR
60 70 80 90 100
VPANSTSNIL NRLLVSYDPR IRPNFKGIPV DVVVNIFINS FGSIQETTMD
110 120 130 140 150
YRVNIFLRQK WNDPRLKLPS DFRGSDALTV DPTMYKCLWK PDLFFANEKS
160 170 180 190 200
ANFHDVTQEN ILLFIFRDGD VLVSMRLSIT LSCPLDLTLF PMDTQRCKMQ
210 220 230 240 250
LESFGYTTDD LRFIWQSGDP VQLEKIALPQ FDIKKEDIEY GNCTKYYKGT
260 270 280 290 300
GYYTCVEVIF TLRRQVGFYM MGVYAPTLLI VVLSWLSFWI NPDASAARVP
310 320 330 340 350
LGIFSVLSLA SECTTLAAEL PKVSYVKALD VWLIACLLFG FASLVEYAVV
360 370 380 390 400
QVMLNNPKRV EAEKARIAKA EQAVGKGGNV AKKNTVNGAS TPVHISTLQV
410 420 430 440 450
GETRCKKVCT SKSDLRSNDF SIVGSLPRDF ELSNYDCYGK PIEVNNGLGK
460 470 480 490
SQAKNNKKPP PAKPVIPTAA KRIDLYARAL FPFCFLFFNV IYWSIYL
Length:497
Mass (Da):56,039
Last modified:March 1, 2001 - v1
Checksum:i82F140C115A887E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268376 mRNA. Translation: AAG14347.1.
AF268374
, AF268367, AF268370, AF268369, AF268371, AF268368, AF268372, AF268373 Genomic DNA. Translation: AAG14345.1.
BC123491 mRNA. Translation: AAI23492.1.
RefSeqiNP_776496.1. NM_174071.2.
UniGeneiBt.5975.

Genome annotation databases

GeneIDi281198.
KEGGibta:281198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268376 mRNA. Translation: AAG14347.1.
AF268374
, AF268367, AF268370, AF268369, AF268371, AF268368, AF268372, AF268373 Genomic DNA. Translation: AAG14345.1.
BC123491 mRNA. Translation: AAI23492.1.
RefSeqiNP_776496.1. NM_174071.2.
UniGeneiBt.5975.

3D structure databases

ProteinModelPortaliQ9GJS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029010.

Proteomic databases

PaxDbiQ9GJS9.
PRIDEiQ9GJS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281198.
KEGGibta:281198.

Organism-specific databases

CTDi2743.

Phylogenomic databases

eggNOGiKOG3644. Eukaryota.
ENOG410XP43. LUCA.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ9GJS9.
KOiK05196.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR008060. Glycine_rcpt_B.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PTHR18945:SF29. PTHR18945:SF29. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01677. GLYRBETA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRB_BOVIN
AccessioniPrimary (citable) accession number: Q9GJS9
Secondary accession number(s): Q08DZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.