Reviewed, UniProtKB/Swiss-Prot Q9FZP1 (HPSE3_ARATH)

Names and origin

Protein names

Recommended name:
    Heparanase-like protein 3
    EC=3.2.-.-

Gene names

Ordered Locus Names:	At5g34940
ORF Names:	T2L5.6, T2L5.7, MGG23.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans By similarity.
Subcellular location	Lysosome membrane; Peripheral membrane protein By similarity. Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 79 family.
Sequence caution	The sequence AAC62790.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAC62794.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB10787.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Lysosome Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FZP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FZP1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     382-382: S → R
     383-536: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 536	512	Heparanase-like protein 3		PRO_0000042271
Sites
	Active site	202	1	Proton donor Potential
	Active site	319	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Glycosylation	510	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	382	1	S → R in isoform 2.		VSP_018141
	Alternative sequence	383 – 536	154	Missing in isoform 2.		VSP_018142

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: BBD6C47CA17DF1C7	FASTA	536	59,709
	10 20 30 40 50 60 MAYRQILAIV LFLCVFQFLD CTVSSAVEEN GTVFVYGRAA VGTIDEDFIC ATLDWWPPEK 70 80 90 100 110 120 CDYGSCSWDH ASILNLDLNN VILQNAIKAF APLKIRIGGT LQDIVIYETP DSKQPCLPFT 130 140 150 160 170 180 KNSSILFGYT QGCLPMRRWD ELNAFFRKTG TKVIFGLNAL SGRSIKSNGE AIGAWNYTNA 190 200 210 220 230 240 ESFIRFTAEN NYTIDGWELG NELCGSGVGA RVGANQYAID TINLRNIVNR VYKNVSPMPL 250 260 270 280 290 300 VIGPGGFFEV DWFTEYLNKA ENSLNATTRH IYDLGPGVDE HLIEKILNPS YLDQEAKSFR 310 320 330 340 350 360 SLKNIIKNSS TKAVAWVGES GGAYNSGRNL VSNAFVYSFW YLDQLGMASL YDTKTYCRQS 370 380 390 400 410 420 LIGGNYGLLN TTNFTPNPDY YSALIWRQLM GRKALFTTFS GTKKIRSYTH CARQSKGITV 430 440 450 460 470 480 LLMNLDNTTT VVAKVELNNS FSLRHTKHMK SYKRASSQLF GGPNGVIQRE EYHLTAKDGN 490 500 510 520 530 LHSQTMLLNG NALQVNSMGD LPPIEPIHIN STEPITIAPY SIVFVHMRNV VVPACA
	Isoform 2. Checksum: EA2A5D5B6DA27268	FASTA	382	42,649
	10 20 30 40 50 60 MAYRQILAIV LFLCVFQFLD CTVSSAVEEN GTVFVYGRAA VGTIDEDFIC ATLDWWPPEK 70 80 90 100 110 120 CDYGSCSWDH ASILNLDLNN VILQNAIKAF APLKIRIGGT LQDIVIYETP DSKQPCLPFT 130 140 150 160 170 180 KNSSILFGYT QGCLPMRRWD ELNAFFRKTG TKVIFGLNAL SGRSIKSNGE AIGAWNYTNA 190 200 210 220 230 240 ESFIRFTAEN NYTIDGWELG NELCGSGVGA RVGANQYAID TINLRNIVNR VYKNVSPMPL 250 260 270 280 290 300 VIGPGGFFEV DWFTEYLNKA ENSLNATTRH IYDLGPGVDE HLIEKILNPS YLDQEAKSFR 310 320 330 340 350 360 SLKNIIKNSS TKAVAWVGES GGAYNSGRNL VSNAFVYSFW YLDQLGMASL YDTKTYCRQS 370 380 LIGGNYGLLN TTNFTPNPDY YR

References

[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF096371 Genomic DNA. Translation: AAC62790.1. Sequence problems. AF096371 Genomic DNA. Translation: AAC62794.1. Sequence problems. AB028613 Genomic DNA. Translation: BAB10787.1. Sequence problems. AK229275 mRNA. Translation: BAF01139.1.
IPI	IPI00523327. IPI00759229.
PIR	T01953. T01954.
RefSeq	NP_851093.1.
UniGene	At.30627
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH79. Glycoside Hydrolase Family 79.
Proteomic databases
PRIDE	Q9FZP1.
Genome annotation databases
GeneID	833437.
GenomeReviews	Gene locus AT5G34940 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.25201.
Organism-specific databases
TAIR	At5g34940.
Phylogenomic databases
OMA	Q9FZP1. WFTELIS.
Family and domain databases
InterPro	IPR005199. Glyco_hydro_79_N. [Graphical view]
PANTHER	PTHR14363. Glyco_hydro_79_N. 1 hit.
Pfam	PF03662. Glyco_hydro_79n. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPSE3_ARATH

Accession

Primary (citable) accession number: Q9FZP1
Secondary accession number(s): O82604, O82605, Q0WP10

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)