Q9FZL3 (MGDG_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable monogalactosyldiacylglycerol synthase, chloroplastic
Short name=NtMGD1
EC=2.4.1.46

Alternative name(s):
MGDG synthase type A

Gene names

Name:

MGD A

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the major structural component of photosynthetic membranes By similarity.
Catalytic activity	UDP-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.
Subcellular location	Plastid › chloroplast membrane Potential.
Sequence similarities	Belongs to the glycosyltransferase 28 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1,2-diacylglycerol 3-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 113	113	Chloroplast Potential
	Chain	114 – 535	422	Probable monogalactosyldiacylglycerol synthase, chloroplastic		PRO_0000349425

Sequences

	Sequence		Length	Mass (Da)
	Q9FZL3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B1B2067E86EDE477	FASTA	535	59,589
	10 20 30 40 50 60 MMQHSSSVTQ EPTNPFGFVS QVGSFVFNNS CSRVSKSNFT LDSYSNLLSN YLYFDCNVKK 70 80 90 100 110 120 DSLNHKNKPK ASPLLSLSIS NKSASSFSRV LFQFNKAIRF HCEKIPLGFA SVGVNCGESN 130 140 150 160 170 180 GVREEGSVVE NEGIPDNGVE SEPPKKVLIL MSDTGGGHRA SAEAIRSAFN EEFGDKYQVF 190 200 210 220 230 240 ITDLWTEHTP WPFNQLPRSY NFLVKHGSLW RMTYYATAPR LVHQTNFAAT STFIAREVAK 250 260 270 280 290 300 GLMKYQPDII ISVHPLMQHV PLRILRSKGL LKKIIFTTVI TDLSTCHPTW FHKLVTRCYC 310 320 330 340 350 360 PSEEVAKRAL RAGLKPYQLK VYGLPVRPSF VKPVPPKVEL RKELGMEEHL PAVLLMGGGE 370 380 390 400 410 420 GMGPIEATAR ALGDALYDEI HGEPIGQVLV ICGRNKKLFN RLTSVQWKIP VQVKGFVTKM 430 440 450 460 470 480 EECMGACDCI ITKAGPGTIA EAVIRGLPII LNDYIAGQEA GNVPYVIENG CGKFSKSPKK 490 500 510 520 530 IANIVAQWFG PRQDELRIMS QNALRLARPD AVFKIVHDMH ELVRQRNFEP QCCPA

References

[1]

"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana." Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J. Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed: 11553816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB047476 mRNA. Translation: BAB11980.1.
3D structure databases
ProteinModelPortal	Q9FZL3.
ModBase	Search...
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGDG_TOBAC

Accession

Primary (citable) accession number: Q9FZL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2001
Last modified:	March 8, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program