Q9FZJ1 (IRX10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,4-xylosyltransferase IRX10
EC=2.4.2.-

Alternative name(s):
Glucuronoxylan glucuronosyltransferase 1
Short name=AtGUT1
Glucuronoxylan glucuronosyltransferase 2
Protein IRREGULAR XYLEM 10
Xylan xylosyltransferase IRX10

Gene names

Name:	IRX10
Synonyms:	GUT1, GUT2
Ordered Locus Names:	At1g27440
ORF Names:	F17L21.23

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. Ref.6 Ref.7
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in xylem cells of roots. Ref.7
Disruption phenotype	Moderate IRX phenotype. Slight reduction of secondary wall thickness and xylan content in cell wall. Ref.5 Ref.6
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from mutant phenotype Ref.6. Source: TAIR secondary cell wall biogenesis Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucuronoxylan glucuronosyltransferase activity Inferred from mutant phenotype Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Probable beta-1,4-xylosyltransferase IRX10		PRO_0000407576
Regions
	Transmembrane	1 – 21	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	22 – 412	391	Lumenal Potential
Amino acid modifications
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FZJ1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 37F694D052F80D39	FASTA	412	46,843
	10 20 30 40 50 60 MKIHSCLSAI LLFLFFSASS AKQNVRTERI SGSAGDVLED DPVGKLKVYV YELPSKYNKK 70 80 90 100 110 120 LLQKDPRCLT HMFAAEIFMH RFLLSSPVRT RNPDEADWFY TPIYPTCDLT PTGLPLPFKS 130 140 150 160 170 180 PRMMRSSIQL ISSNWPYWNR TEGADHFFVV PHDFGACFHY QEEKAIERGI LPLLQRATLV 190 200 210 220 230 240 QTFGQRNHVC LDEGSITIPP FAPPQKMQAH FIPPDIPRSI FVYFRGLFYD VNNDPEGGYY 250 260 270 280 290 300 ARGARAAVWE NFKNNPLFDI STDHPTTYYE DMQRAIFCLC PLGWAPWSPR LVEAVVFGCI 310 320 330 340 350 360 PVIIADDIVL PFADAIPWEE IGVFVAEKDV PELDTILTSI PTEVILRKQR LLANPSMKRA 370 380 390 400 410 MLFPQPAQPG DAFHQILNGL ARKLPHDKSI YLKTGEKALN WTAGPVADLK PW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics." Brown D.M., Zeef L.A.H., Ellis J., Goodacre R., Turner S.R. Plant Cell 17:2281-2295(2005) [PubMed: 15980264] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis." Brown D.M., Zhang Z., Stephens E., Dupree P., Turner S.R. Plant J. 57:732-746(2009) [PubMed: 18980662] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan." Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P., Marchant A. Plant Physiol. 153:542-554(2010) [PubMed: 20424005] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004557 Genomic DNA. Translation: AAF99743.1. CP002684 Genomic DNA. Translation: AEE30830.1. BT022053 mRNA. Translation: AAY25465.1. BT025555 mRNA. Translation: ABF58973.1.
IPI	IPI00546884.
RefSeq	NP_174064.1. NM_102508.2.
UniGene	At.49923.
3D structure databases
ProteinModelPortal	Q9FZJ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FZJ1.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q9FZJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G27440.1; AT1G27440.1; AT1G27440.
GeneID	839635.
GenomeReviews	Gene locus AT1G27440 in contig CT485782_GR.
KEGG	ath:AT1G27440.
NMPDR	fig|3702.1.peg.3071.
Organism-specific databases
TAIR	At1g27440.
Phylogenomic databases
eggNOG	KOG1021.
GeneTree	EPGT00070000028323.
HOGENOM	HBG319192.
InParanoid	Q9FZJ1.
OMA	RNPDEAD.
PhylomeDB	Q9FZJ1.
ProtClustDB	CLSN2682887.
Gene expression databases
Genevestigator	Q9FZJ1.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IRX10_ARATH

Accession

Primary (citable) accession number: Q9FZJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program