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Q9FZH5

- MPI2_ARATH

UniProt

Q9FZH5 - MPI2_ARATH

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Protein
Mannose-6-phosphate isomerase 2
Gene
PMI2, DIN9, At1g67070, F1O19.12
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.1 Publication

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Inhibited by EDTA, Zn2+, Cd2+, DTT, p-chloromercuribenzoate and L-ascorbic acid (AsA).1 Publication

Kineticsi

  1. KM=372 µM for mannose-6-phosphate1 Publication

Vmax=22.5 µmol/min/mg enzyme with mannose-6-phosphate as substrate

pH dependencei

Optimum pH is 7.5.

Temperature dependencei

Optimum temperature is 48 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Zinc By similarity
Metal bindingi133 – 1331Zinc By similarity
Metal bindingi158 – 1581Zinc By similarity
Metal bindingi296 – 2961Zinc By similarity
Active sitei315 – 3151 By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: TAIR
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  2. L-ascorbic acid biosynthetic process Source: TAIR
  3. response to DDT Source: UniProtKB
  4. response to L-ascorbic acid Source: UniProtKB
  5. response to absence of light Source: UniProtKB
  6. response to cadmium ion Source: UniProtKB
  7. response to sucrose Source: UniProtKB
  8. response to zinc ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G67070-MONOMER.
MetaCyc:AT1G67070-MONOMER.
ReactomeiREACT_190862. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase 2 (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase 2
Phosphomannose isomerase 2
Short name:
PMI2
Protein DARK INDUCIBLE 9
Gene namesi
Name:PMI2
Synonyms:DIN9
Ordered Locus Names:At1g67070
ORF Names:F1O19.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G67070.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Mannose-6-phosphate isomerase 2
PRO_0000420340Add
BLAST

Proteomic databases

PRIDEiQ9FZH5.

Expressioni

Tissue specificityi

Not expressed in any organs under light (at protein level).1 Publication

Developmental stagei

Expressed at the last stage of senescence in old leaves.2 Publications

Inductioni

By sugar starvation, by dark and by 3-O-methyl-Glc (3-OMG). Down-regulated by sugars. Up-regulated by DCMU, an exogenous photosynthesis inhibitor.4 Publications

Gene expression databases

ArrayExpressiQ9FZH5.
GenevestigatoriQ9FZH5.

Structurei

3D structure databases

ProteinModelPortaliQ9FZH5.
SMRiQ9FZH5. Positions 20-423.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000241277.
InParanoidiQ9FZH5.
KOiK01809.
OMAiPYAEFWV.
PhylomeDBiQ9FZH5.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FZH5-1 [UniParc]FASTAAdd to Basket

« Hide

MGADAIQTNG HDQAKLTGGE EIQRLRCFVK NYEWGKLGPE SLVARLQEAN    50
TGQRVDSEIP YAEFWMGTHE SGPSHVEFGS GHGVSDKCMV TLKSWVLDNP 100
NLLGSKVVDK WGCDLPFLFK VLSVTKALSI QAHPNKALAE KLHREDPLLY 150
RDNNHKPEIA LAVTPFQALC GFVTLKELKE VITNVPEITE LVGSKAADQI 200
FNVHEHDEDE RIKSVVRLIF TQLMSASNNE TKQVVSRMKN RLLLETKHRE 250
LSEKEKLVLE LEKQYTGDIG VISAFFFNYV KLNPGEALYL DANEPHAYIS 300
GDCVECMAAS DNVVRAGLTP KHRDVQTLCS MLTYKLGYPE ILKGFPLTPY 350
VTRYLPPFDE FEVDHCDLPR GKSTVFPAVP GPSVYLVIEG KGQLRTGSSK 400
VLVNRGDVLF VPADIEIHVT GESDVMKLYR AGVSSRFFQT L 441
Length:441
Mass (Da):49,248
Last modified:March 1, 2001 - v1
Checksum:i05D6F0820858D2B1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851S → F in AAG23720. 1 Publication
Sequence conflicti90 – 901V → A in AAG23720. 1 Publication
Sequence conflicti211 – 2111R → S in AAG23720. 1 Publication
Sequence conflicti288 – 2881L → W in BAD44147. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007152 Genomic DNA. Translation: AAF98217.1.
CP002684 Genomic DNA. Translation: AEE34592.1.
AK176384 mRNA. Translation: BAD44147.1.
AK176481 mRNA. Translation: BAD44244.1.
BT025995 mRNA. Translation: ABG25084.1.
AF159377 mRNA. Translation: AAG23720.1.
PIRiE96694.
RefSeqiNP_176878.1. NM_105377.4.
UniGeneiAt.35695.

Genome annotation databases

EnsemblPlantsiAT1G67070.1; AT1G67070.1; AT1G67070.
GeneIDi843027.
KEGGiath:AT1G67070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007152 Genomic DNA. Translation: AAF98217.1 .
CP002684 Genomic DNA. Translation: AEE34592.1 .
AK176384 mRNA. Translation: BAD44147.1 .
AK176481 mRNA. Translation: BAD44244.1 .
BT025995 mRNA. Translation: ABG25084.1 .
AF159377 mRNA. Translation: AAG23720.1 .
PIRi E96694.
RefSeqi NP_176878.1. NM_105377.4.
UniGenei At.35695.

3D structure databases

ProteinModelPortali Q9FZH5.
SMRi Q9FZH5. Positions 20-423.
ModBasei Search...

Proteomic databases

PRIDEi Q9FZH5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G67070.1 ; AT1G67070.1 ; AT1G67070 .
GeneIDi 843027.
KEGGi ath:AT1G67070.

Organism-specific databases

TAIRi AT1G67070.

Phylogenomic databases

HOGENOMi HOG000241277.
InParanoidi Q9FZH5.
KOi K01809.
OMAi PYAEFWV.
PhylomeDBi Q9FZH5.

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00423 .
BioCyci ARA:AT1G67070-MONOMER.
MetaCyc:AT1G67070-MONOMER.
Reactomei REACT_190862. Synthesis of GDP-mannose.

Miscellaneous databases

PROi Q9FZH5.

Gene expression databases

ArrayExpressi Q9FZH5.
Genevestigatori Q9FZH5.

Family and domain databases

Gene3Di 2.60.120.10. 3 hits.
InterProi IPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR10309. PTHR10309. 1 hit.
Pfami PF01238. PMI_typeI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSi PR00714. MAN6PISMRASE.
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR00218. manA. 1 hit.
PROSITEi PS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars."
    Fujiki Y., Yoshikawa Y., Sato T., Inada N., Ito M., Nishida I., Watanabe A.
    Physiol. Plantarum 111:345-352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-240, INDUCTION BY DARK, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  6. "Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of Arabidopsis."
    Fujiki Y., Ito M., Nishida I., Watanabe A.
    Plant Physiol. 124:1139-1148(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Response to darkness of late-responsive dark-inducible genes is positively regulated by leaf age and negatively regulated by calmodulin-antagonist-sensitive signalling in Arabidopsis thaliana."
    Fujiki Y., Nakagawa Y., Furumoto T., Yoshida S., Biswal B., Ito M., Watanabe A., Nishida I.
    Plant Cell Physiol. 46:1741-1746(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY DARK, DEVELOPMENTAL STAGE.
  8. "Arabidopsis phosphomannose isomerase 1, but not phosphomannose isomerase 2, is essential for ascorbic acid biosynthesis."
    Maruta T., Yonemitsu M., Yabuta Y., Tamoi M., Ishikawa T., Shigeoka S.
    J. Biol. Chem. 283:28842-28851(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiMPI2_ARATH
AccessioniPrimary (citable) accession number: Q9FZH5
Secondary accession number(s): Q67YT4, Q9FVM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi