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Protein

Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

ALG9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Adds the seventh and the ninth mannose residues in an alpha-1,2 linkage onto the dolichol-PP-oligosaccharide precursors dolichol-PP-Man6GlcNAc2 and dolichol-PP-Man8GlcNAc2.1 Publication

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication
Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: TAIR
  • oligosaccharide-lipid intermediate biosynthetic process Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Kinase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G16900-MONOMER.
BRENDAi2.4.1.259. 399.
2.4.1.261. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.259)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG9
Asparagine-linked glycosylation protein 9
Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.261)
Gene namesi
Name:ALG9
Ordered Locus Names:At1g16900
ORF Names:F17F16.20, F6I1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G16900.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei129 – 14921HelicalSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Transmembranei208 – 22821HelicalSequence analysisAdd
BLAST
Transmembranei237 – 25721HelicalSequence analysisAdd
BLAST
Transmembranei297 – 31721HelicalSequence analysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000412587Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FZ49.
PRIDEiQ9FZ49.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FZ49. baseline and differential.
GenevisibleiQ9FZ49. AT.

Interactioni

Protein-protein interaction databases

BioGridi23501. 1 interaction.
IntActiQ9FZ49. 1 interaction.
STRINGi3702.AT1G16900.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FZ49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 218Poly-Ser
Compositional biasi257 – 2604Poly-Tyr

Sequence similaritiesi

Belongs to the glycosyltransferase 22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2515. Eukaryota.
ENOG410XP6D. LUCA.
HOGENOMiHOG000205434.
InParanoidiQ9FZ49.
KOiK03846.
OMAiAPLNIVW.
OrthoDBiEOG093608G9.
PhylomeDBiQ9FZ49.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FZ49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLTTTRQRR PLISDSSSSS STKSYSKTDK PGRSNGGDAE DGGLRWFLPF
60 70 80 90 100
IALCYLRYMS ATSNIIHDCD EVFNYWEPLH YILYKSGFQT WEYSSNFALR
110 120 130 140 150
SYLYILFHEL AGRPASWWFG DDKVRVFYAV RLFLGLVSAV SDTVLVVALS
160 170 180 190 200
RKYGKRIATY AVAMLCLTSG CFFASTSFLP SSFSMYAISL SSGLLLFEKY
210 220 230 240 250
AMAVAVSVVG VILGWPFSIL AFLPVVIYSL VKRFKQAFIA GAVTTIFLLG
260 270 280 290 300
VSLLVDYYYY KRWTSSVLNL LIYNVLGGGE SHLYGTEGAL FYIRNGFNNF
310 320 330 340 350
NFCFILAMLF VAIYPVIRRK YDRALLVVIS PMYIWLAFMS LQPHKEERFL
360 370 380 390 400
YPIYPLICVS ASAVIENIPE LFREKYSSRE SLLVTITKYM RPVILGCILC
410 420 430 440 450
ASHSRTFALI NGYSAPLEVY KLLEHHDDAG PGSVLCVGSE WHRYPSSFFV
460 470 480 490 500
PHYISEVRWI DDGFRGLLPF PFNNTLGGTS ASPPYFNNKN QASEEQYLKN
510 520 530 540 550
IETCTFLIEL QLSRPYQYRG SDLSTWEAIA VLPYLDRELS PAKYRSFFIP
560 570
HMWQEKNVFG KYVALRRVPK
Length:570
Mass (Da):65,083
Last modified:March 1, 2001 - v1
Checksum:iCE6885EA115D0997
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC051629 Genomic DNA. Translation: AAF99843.1.
CP002684 Genomic DNA. Translation: AEE29518.1.
AY140061 mRNA. Translation: AAM98202.1.
BT010395 mRNA. Translation: AAQ56838.1.
PIRiD86304.
RefSeqiNP_173134.2. NM_101551.3.
UniGeneiAt.41864.

Genome annotation databases

EnsemblPlantsiAT1G16900.1; AT1G16900.1; AT1G16900.
GeneIDi838261.
GrameneiAT1G16900.1; AT1G16900.1; AT1G16900.
KEGGiath:AT1G16900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC051629 Genomic DNA. Translation: AAF99843.1.
CP002684 Genomic DNA. Translation: AEE29518.1.
AY140061 mRNA. Translation: AAM98202.1.
BT010395 mRNA. Translation: AAQ56838.1.
PIRiD86304.
RefSeqiNP_173134.2. NM_101551.3.
UniGeneiAt.41864.

3D structure databases

ProteinModelPortaliQ9FZ49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23501. 1 interaction.
IntActiQ9FZ49. 1 interaction.
STRINGi3702.AT1G16900.1.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Proteomic databases

PaxDbiQ9FZ49.
PRIDEiQ9FZ49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G16900.1; AT1G16900.1; AT1G16900.
GeneIDi838261.
GrameneiAT1G16900.1; AT1G16900.1; AT1G16900.
KEGGiath:AT1G16900.

Organism-specific databases

TAIRiAT1G16900.

Phylogenomic databases

eggNOGiKOG2515. Eukaryota.
ENOG410XP6D. LUCA.
HOGENOMiHOG000205434.
InParanoidiQ9FZ49.
KOiK03846.
OMAiAPLNIVW.
OrthoDBiEOG093608G9.
PhylomeDBiQ9FZ49.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT1G16900-MONOMER.
BRENDAi2.4.1.259. 399.
2.4.1.261. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ9FZ49.

Gene expression databases

ExpressionAtlasiQ9FZ49. baseline and differential.
GenevisibleiQ9FZ49. AT.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG9_ARATH
AccessioniPrimary (citable) accession number: Q9FZ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG9 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.