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Q9FZ48

- UBC36_ARATH

UniProt

Q9FZ48 - UBC36_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 36

Gene

UBC36

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G16890-MONOMER.
ARA:GQT-2517-MONOMER.
ARA:GQT-718-MONOMER.
ReactomeiREACT_247278. ISG15 antiviral mechanism.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 36 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 36
Gene namesi
Name:UBC36
Synonyms:UBC13B, UBG13B
Ordered Locus Names:At1g16890
ORF Names:F17F16.19, F6I1.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G16890.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Ubiquitin-conjugating enzyme E2 36PRO_0000345201Add
BLAST

Proteomic databases

PaxDbiQ9FZ48.
PRIDEiQ9FZ48.

Expressioni

Tissue specificityi

Ubiquitously expressed at low level.1 Publication

Inductioni

Not induced by iron.2 Publications

Gene expression databases

ExpressionAtlasiQ9FZ48. baseline.
GenevestigatoriQ9FZ48.

Interactioni

Subunit structurei

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

Protein-protein interaction databases

BioGridi23500. 10 interactions.
IntActiQ9FZ48. 9 interactions.
STRINGi3702.AT1G16890.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FZ48.
SMRiQ9FZ48. Positions 6-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ9FZ48.
KOiK10580.
PhylomeDBiQ9FZ48.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FZ48-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSNLPRRI IKETQRLLSE PAPGISASPS EENMRYFNVM ILGPTQSPYE
60 70 80 90 100
GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA
110 120 130 140 150
LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVE TAKEWTRLYA

SGA
Length:153
Mass (Da):17,220
Last modified:March 1, 2001 - v1
Checksum:i66FD614EA346EDD1
GO
Isoform 2 (identifier: Q9FZ48-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Show »
Length:120
Mass (Da):13,631
Checksum:iA9498B1FE3F0AD28
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3333Missing in isoform 2. CuratedVSP_034932Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027049 mRNA. Translation: AAY44875.1.
AC051629 Genomic DNA. Translation: AAF99844.1.
CP002684 Genomic DNA. Translation: AEE29515.1.
CP002684 Genomic DNA. Translation: AEE29516.1.
AY052307 mRNA. Translation: AAK96500.1.
AY061926 mRNA. Translation: AAL31253.1.
AY086780 mRNA. Translation: AAM63831.1.
PIRiC86304.
RefSeqiNP_564011.1. NM_101550.3. [Q9FZ48-1]
NP_849678.1. NM_179347.1. [Q9FZ48-2]
UniGeneiAt.16052.
At.24697.

Genome annotation databases

EnsemblPlantsiAT1G16890.2; AT1G16890.2; AT1G16890. [Q9FZ48-1]
GeneIDi838260.
KEGGiath:AT1G16890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027049 mRNA. Translation: AAY44875.1 .
AC051629 Genomic DNA. Translation: AAF99844.1 .
CP002684 Genomic DNA. Translation: AEE29515.1 .
CP002684 Genomic DNA. Translation: AEE29516.1 .
AY052307 mRNA. Translation: AAK96500.1 .
AY061926 mRNA. Translation: AAL31253.1 .
AY086780 mRNA. Translation: AAM63831.1 .
PIRi C86304.
RefSeqi NP_564011.1. NM_101550.3. [Q9FZ48-1 ]
NP_849678.1. NM_179347.1. [Q9FZ48-2 ]
UniGenei At.16052.
At.24697.

3D structure databases

ProteinModelPortali Q9FZ48.
SMRi Q9FZ48. Positions 6-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 23500. 10 interactions.
IntActi Q9FZ48. 9 interactions.
STRINGi 3702.AT1G16890.2-P.

Proteomic databases

PaxDbi Q9FZ48.
PRIDEi Q9FZ48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G16890.2 ; AT1G16890.2 ; AT1G16890 . [Q9FZ48-1 ]
GeneIDi 838260.
KEGGi ath:AT1G16890.

Organism-specific databases

TAIRi AT1G16890.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi Q9FZ48.
KOi K10580.
PhylomeDBi Q9FZ48.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G16890-MONOMER.
ARA:GQT-2517-MONOMER.
ARA:GQT-718-MONOMER.
Reactomei REACT_247278. ISG15 antiviral mechanism.

Gene expression databases

ExpressionAtlasi Q9FZ48. baseline.
Genevestigatori Q9FZ48.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, GENE FAMILY, NOMENCLATURE.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants."
    Wen R., Newton L., Li G., Wang H., Xiao W.
    Plant Mol. Biol. 61:241-253(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YEAST AND HUMAN MMS2, TISSUE SPECIFICITY, INDUCTION.
  7. Cited for: INTERACTION WITH RGLG2.
  8. "Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response."
    Wen R., Torres-Acosta J.A., Pastushok L., Lai X., Pelzer L., Wang H., Xiao W.
    Plant Cell 20:213-227(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UEV1A; UEV1B; UEV1C AND UEV1D.
  9. "A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots."
    Li W., Schmidt W.
    Plant J. 62:330-343(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY IRON.

Entry informationi

Entry nameiUBC36_ARATH
AccessioniPrimary (citable) accession number: Q9FZ48
Secondary accession number(s): Q3EDA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Partly functionally redundant with UBC35.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3