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Q9FZ48

- UBC36_ARATH

UniProt

Q9FZ48 - UBC36_ARATH

Protein

Ubiquitin-conjugating enzyme E2 36

Gene

UBC36

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G16890-MONOMER.
    ARA:GQT-2517-MONOMER.
    ARA:GQT-718-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 36 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 36
    Gene namesi
    Name:UBC36
    Synonyms:UBC13B, UBG13B
    Ordered Locus Names:At1g16890
    ORF Names:F17F16.19, F6I1.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G16890.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 153153Ubiquitin-conjugating enzyme E2 36PRO_0000345201Add
    BLAST

    Proteomic databases

    PaxDbiQ9FZ48.
    PRIDEiQ9FZ48.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed at low level.1 Publication

    Inductioni

    Not induced by iron.2 Publications

    Gene expression databases

    GenevestigatoriQ9FZ48.

    Interactioni

    Subunit structurei

    Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

    Protein-protein interaction databases

    BioGridi23500. 10 interactions.
    IntActiQ9FZ48. 9 interactions.
    STRINGi3702.AT1G16890.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FZ48.
    SMRiQ9FZ48. Positions 6-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ9FZ48.
    KOiK10580.
    PhylomeDBiQ9FZ48.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FZ48-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANSNLPRRI IKETQRLLSE PAPGISASPS EENMRYFNVM ILGPTQSPYE    50
    GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA 100
    LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVE TAKEWTRLYA 150
    SGA 153
    Length:153
    Mass (Da):17,220
    Last modified:March 1, 2001 - v1
    Checksum:i66FD614EA346EDD1
    GO
    Isoform 2 (identifier: Q9FZ48-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-33: Missing.

    Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:120
    Mass (Da):13,631
    Checksum:iA9498B1FE3F0AD28
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3333Missing in isoform 2. CuratedVSP_034932Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027049 mRNA. Translation: AAY44875.1.
    AC051629 Genomic DNA. Translation: AAF99844.1.
    CP002684 Genomic DNA. Translation: AEE29515.1.
    CP002684 Genomic DNA. Translation: AEE29516.1.
    AY052307 mRNA. Translation: AAK96500.1.
    AY061926 mRNA. Translation: AAL31253.1.
    AY086780 mRNA. Translation: AAM63831.1.
    PIRiC86304.
    RefSeqiNP_564011.1. NM_101550.3. [Q9FZ48-1]
    NP_849678.1. NM_179347.1. [Q9FZ48-2]
    UniGeneiAt.16052.
    At.24697.

    Genome annotation databases

    EnsemblPlantsiAT1G16890.2; AT1G16890.2; AT1G16890. [Q9FZ48-1]
    GeneIDi838260.
    KEGGiath:AT1G16890.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027049 mRNA. Translation: AAY44875.1 .
    AC051629 Genomic DNA. Translation: AAF99844.1 .
    CP002684 Genomic DNA. Translation: AEE29515.1 .
    CP002684 Genomic DNA. Translation: AEE29516.1 .
    AY052307 mRNA. Translation: AAK96500.1 .
    AY061926 mRNA. Translation: AAL31253.1 .
    AY086780 mRNA. Translation: AAM63831.1 .
    PIRi C86304.
    RefSeqi NP_564011.1. NM_101550.3. [Q9FZ48-1 ]
    NP_849678.1. NM_179347.1. [Q9FZ48-2 ]
    UniGenei At.16052.
    At.24697.

    3D structure databases

    ProteinModelPortali Q9FZ48.
    SMRi Q9FZ48. Positions 6-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23500. 10 interactions.
    IntActi Q9FZ48. 9 interactions.
    STRINGi 3702.AT1G16890.2-P.

    Proteomic databases

    PaxDbi Q9FZ48.
    PRIDEi Q9FZ48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G16890.2 ; AT1G16890.2 ; AT1G16890 . [Q9FZ48-1 ]
    GeneIDi 838260.
    KEGGi ath:AT1G16890.

    Organism-specific databases

    TAIRi AT1G16890.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q9FZ48.
    KOi K10580.
    PhylomeDBi Q9FZ48.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT1G16890-MONOMER.
    ARA:GQT-2517-MONOMER.
    ARA:GQT-718-MONOMER.

    Gene expression databases

    Genevestigatori Q9FZ48.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, GENE FAMILY, NOMENCLATURE.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants."
      Wen R., Newton L., Li G., Wang H., Xiao W.
      Plant Mol. Biol. 61:241-253(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH YEAST AND HUMAN MMS2, TISSUE SPECIFICITY, INDUCTION.
    7. Cited for: INTERACTION WITH RGLG2.
    8. "Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response."
      Wen R., Torres-Acosta J.A., Pastushok L., Lai X., Pelzer L., Wang H., Xiao W.
      Plant Cell 20:213-227(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UEV1A; UEV1B; UEV1C AND UEV1D.
    9. "A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots."
      Li W., Schmidt W.
      Plant J. 62:330-343(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY IRON.

    Entry informationi

    Entry nameiUBC36_ARATH
    AccessioniPrimary (citable) accession number: Q9FZ48
    Secondary accession number(s): Q3EDA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Partly functionally redundant with UBC35.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3