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Protein

Ubiquitin-conjugating enzyme E2 36

Gene

UBC36

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.3 Publications

Miscellaneous

Partly functionally redundant with UBC35.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-9020702 Interleukin-1 signaling
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 36 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 36
Ubiquitin carrier protein 36
Gene namesi
Name:UBC36
Synonyms:UBC13B, UBG13B
Ordered Locus Names:At1g16890
ORF Names:F17F16.19, F6I1.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G16890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003452011 – 153Ubiquitin-conjugating enzyme E2 36Add BLAST153

Proteomic databases

PRIDEiQ9FZ48

Expressioni

Tissue specificityi

Ubiquitously expressed at low level.1 Publication

Inductioni

Not induced by iron.2 Publications

Gene expression databases

ExpressionAtlasiQ9FZ48 baseline and differential
GenevisibleiQ9FZ48 AT

Interactioni

Subunit structurei

Interacts with yeast and human Mms2, with the RING domain of RGLG2 and with UEV1A, UEV1B, UEV1C and UEV1D.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi23500, 11 interactors
IntActiQ9FZ48, 9 interactors

Structurei

3D structure databases

ProteinModelPortaliQ9FZ48
SMRiQ9FZ48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
InParanoidiQ9FZ48
KOiK10580
PhylomeDBiQ9FZ48

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FZ48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANSNLPRRI IKETQRLLSE PAPGISASPS EENMRYFNVM ILGPTQSPYE
60 70 80 90 100
GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA
110 120 130 140 150
LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVE TAKEWTRLYA

SGA
Length:153
Mass (Da):17,220
Last modified:March 1, 2001 - v1
Checksum:i66FD614EA346EDD1
GO
Isoform 2 (identifier: Q9FZ48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:120
Mass (Da):13,631
Checksum:iA9498B1FE3F0AD28
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0349321 – 33Missing in isoform 2. CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027049 mRNA Translation: AAY44875.1
AC051629 Genomic DNA Translation: AAF99844.1
CP002684 Genomic DNA Translation: AEE29515.1
CP002684 Genomic DNA Translation: AEE29516.1
AY052307 mRNA Translation: AAK96500.1
AY061926 mRNA Translation: AAL31253.1
AY086780 mRNA Translation: AAM63831.1
PIRiC86304
RefSeqiNP_564011.1, NM_101550.4 [Q9FZ48-1]
NP_849678.1, NM_179347.2 [Q9FZ48-2]
UniGeneiAt.16052
At.24697

Genome annotation databases

EnsemblPlantsiAT1G16890.1; AT1G16890.1; AT1G16890 [Q9FZ48-2]
AT1G16890.2; AT1G16890.2; AT1G16890 [Q9FZ48-1]
GeneIDi838260
GrameneiAT1G16890.1; AT1G16890.1; AT1G16890 [Q9FZ48-2]
AT1G16890.2; AT1G16890.2; AT1G16890 [Q9FZ48-1]
KEGGiath:AT1G16890

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC36_ARATH
AccessioniPrimary (citable) accession number: Q9FZ48
Secondary accession number(s): Q3EDA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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