Q9FZ36 (M3K2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 2
EC=2.7.11.25

Alternative name(s):
Arabidopsis NPK1-related protein kinase 2

Gene names

Name:	ANP2
Ordered Locus Names:	At1g54960
ORF Names:	T24C10.7, F14C21.49

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm › cytoskeleton Probable.
Tissue specificity	Expressed in roots and flowers. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAG51109.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cortical microtubule organization Inferred from mutant phenotype Ref.3. Source: UniProtKB cytokinesis Inferred from genetic interaction. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Mitogen-activated protein kinase kinase kinase 2		PRO_0000086271
Regions
	Domain	68 – 330	263	Protein kinase
	Nucleotide binding	74 – 82	9	ATP By similarity
	Coiled coil	105 – 130	26	Potential
	Coiled coil	605 – 628	24	Potential
	Compositional bias	405 – 411	7	Poly-Asp
Sites
	Active site	196	1	Proton acceptor By similarity
	Binding site	97	1	ATP By similarity
Experimental info
	Sequence conflict	99	1	V → A in BAA21856. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ36 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0204655E1F9E918E	FASTA	651	71,849
	10 20 30 40 50 60 MQDLFGSVRR SLVFRSTTDD ENQENHPPPF PSLLADKITS CIRKSMVFAK SQSPPNNSTV 70 80 90 100 110 120 QIKPPIRWRK GQLIGRGAFG TVYMGMNLDS GELLAVKQVL ITSNCASKEK TQAHIQELEE 130 140 150 160 170 180 EVKLLKNLSH PNIVRYLGTV REDETLNILL EFVPGGSISS LLEKFGAFPE SVVRTYTNQL 190 200 210 220 230 240 LLGLEYLHNH AIMHRDIKGA NILVDNQGCI KLADFGASKQ VAELATISGA KSMKGTPYWM 250 260 270 280 290 300 APEVILQTGH SFSADIWSVG CTVIEMVTGK APWSQQYKEI AAIFHIGTTK SHPPIPDNIS 310 320 330 340 350 360 SDANDFLLKC LQQEPNLRPT ASELLKHPFV TGKQKESASK DLTSFMDNSC SPLPSELTNI 370 380 390 400 410 420 TSYQTSTSDD VGDICNLGSL TCTLAFPEKS IQNNSLCLKS NNGYDDDDDN DMCLIDDENF 430 440 450 460 470 480 LTYNGETGPS LDNNTDAKKS CDTMSEISDI LKCKFDENSG NGETETKVSM EVDHPSYSED 490 500 510 520 530 540 ENELTESKIK AFLDDKAAEL KKLQTPLYEE FYNGMITCSP ICMESNINNN KREEAPRGFL 550 560 570 580 590 600 KLPPKSRSPS QGHIGRSPSR ATDAACCSKS PESGNSSGAP KNSNASAGAE QESNSQSVAL 610 620 630 640 650 SEIERKWKEE LDQELERKRR EITRQAGMGS SPRDRSLSRH REKSRFASPG K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)." Nishihama R., Banno H., Kawahara E., Irie K., Machida Y. Plant J. 12:39-48(1997) [PubMed: 9263451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-651, TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization." Beck M., Komis G., Mueller J., Menzel D., Samaj J. Plant Cell 22:755-771(2010) [PubMed: 20215588] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC064840 Genomic DNA. Translation: AAG00876.1. AC069144 Genomic DNA. Translation: AAG51109.1. Sequence problems. AB000798 mRNA. Translation: BAA21856.1.
IPI	IPI00523113.
PIR	A96591.
3D structure databases
ProteinModelPortal	Q9FZ36.
SMR	Q9FZ36. Positions 67-331.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FZ36.
Proteomic databases
PRIDE	Q9FZ36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT1G54960 in contig CT485782_GR.
Organism-specific databases
GeneFarm	862. 89.
TAIR	At1g54960.
Phylogenomic databases
eggNOG	KOG0198.
GeneTree	EPGT00070000028518.
HOGENOM	HBG318239.
InParanoid	Q9FZ36.
PhylomeDB	Q9FZ36.
Gene expression databases
ArrayExpress	Q9FZ36.
Genevestigator	Q9FZ36.
GermOnline	AT1G54960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K2_ARATH

Accession

Primary (citable) accession number: Q9FZ36
Secondary accession number(s): O22041, Q9C7M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program