Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase kinase kinase 2

Gene

ANP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971ATPPROSITE-ProRule annotation
Active sitei196 – 1961Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 829ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cortical microtubule organization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G54960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 2 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related protein kinase 2
Gene namesi
Name:ANP2
Ordered Locus Names:At1g54960
ORF Names:F14C21.49, T24C10.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G54960.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 651651Mitogen-activated protein kinase kinase kinase 2PRO_0000086271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki108 – 108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki110 – 110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9FZ36.
PRIDEiQ9FZ36.

PTM databases

iPTMnetiQ9FZ36.

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Gene expression databases

GenevisibleiQ9FZ36. AT.

Interactioni

Protein-protein interaction databases

BioGridi27162. 1 interaction.
MINTiMINT-8067973.
STRINGi3702.AT1G54960.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FZ36.
SMRiQ9FZ36. Positions 15-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 330263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili105 – 13026Sequence analysisAdd
BLAST
Coiled coili605 – 62824Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi405 – 4117Poly-Asp

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiQ9FZ36.
PhylomeDBiQ9FZ36.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FZ36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDLFGSVRR SLVFRSTTDD ENQENHPPPF PSLLADKITS CIRKSMVFAK
60 70 80 90 100
SQSPPNNSTV QIKPPIRWRK GQLIGRGAFG TVYMGMNLDS GELLAVKQVL
110 120 130 140 150
ITSNCASKEK TQAHIQELEE EVKLLKNLSH PNIVRYLGTV REDETLNILL
160 170 180 190 200
EFVPGGSISS LLEKFGAFPE SVVRTYTNQL LLGLEYLHNH AIMHRDIKGA
210 220 230 240 250
NILVDNQGCI KLADFGASKQ VAELATISGA KSMKGTPYWM APEVILQTGH
260 270 280 290 300
SFSADIWSVG CTVIEMVTGK APWSQQYKEI AAIFHIGTTK SHPPIPDNIS
310 320 330 340 350
SDANDFLLKC LQQEPNLRPT ASELLKHPFV TGKQKESASK DLTSFMDNSC
360 370 380 390 400
SPLPSELTNI TSYQTSTSDD VGDICNLGSL TCTLAFPEKS IQNNSLCLKS
410 420 430 440 450
NNGYDDDDDN DMCLIDDENF LTYNGETGPS LDNNTDAKKS CDTMSEISDI
460 470 480 490 500
LKCKFDENSG NGETETKVSM EVDHPSYSED ENELTESKIK AFLDDKAAEL
510 520 530 540 550
KKLQTPLYEE FYNGMITCSP ICMESNINNN KREEAPRGFL KLPPKSRSPS
560 570 580 590 600
QGHIGRSPSR ATDAACCSKS PESGNSSGAP KNSNASAGAE QESNSQSVAL
610 620 630 640 650
SEIERKWKEE LDQELERKRR EITRQAGMGS SPRDRSLSRH REKSRFASPG

K
Length:651
Mass (Da):71,849
Last modified:March 1, 2001 - v1
Checksum:i0204655E1F9E918E
GO

Sequence cautioni

The sequence AAG51109.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AEE33167.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991V → A in BAA21856 (PubMed:9263451).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC064840 Genomic DNA. Translation: AAG00876.1.
AC069144 Genomic DNA. Translation: AAG51109.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33167.1. Different initiation.
AB000798 mRNA. Translation: BAA21856.1.
PIRiA96591.
RefSeqiNP_175894.4. NM_104370.4.
UniGeneiAt.37136.

Genome annotation databases

EnsemblPlantsiAT1G54960.1; AT1G54960.1; AT1G54960.
GeneIDi841937.
GrameneiAT1G54960.1; AT1G54960.1; AT1G54960.
KEGGiath:AT1G54960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC064840 Genomic DNA. Translation: AAG00876.1.
AC069144 Genomic DNA. Translation: AAG51109.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33167.1. Different initiation.
AB000798 mRNA. Translation: BAA21856.1.
PIRiA96591.
RefSeqiNP_175894.4. NM_104370.4.
UniGeneiAt.37136.

3D structure databases

ProteinModelPortaliQ9FZ36.
SMRiQ9FZ36. Positions 15-473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27162. 1 interaction.
MINTiMINT-8067973.
STRINGi3702.AT1G54960.1.

PTM databases

iPTMnetiQ9FZ36.

Proteomic databases

PaxDbiQ9FZ36.
PRIDEiQ9FZ36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G54960.1; AT1G54960.1; AT1G54960.
GeneIDi841937.
GrameneiAT1G54960.1; AT1G54960.1; AT1G54960.
KEGGiath:AT1G54960.

Organism-specific databases

TAIRiAT1G54960.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiQ9FZ36.
PhylomeDBiQ9FZ36.

Enzyme and pathway databases

BioCyciARA:AT1G54960-MONOMER.

Miscellaneous databases

PROiQ9FZ36.

Gene expression databases

GenevisibleiQ9FZ36. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)."
    Nishihama R., Banno H., Kawahara E., Irie K., Machida Y.
    Plant J. 12:39-48(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-651, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  4. "Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization."
    Beck M., Komis G., Mueller J., Menzel D., Samaj J.
    Plant Cell 22:755-771(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiM3K2_ARATH
AccessioniPrimary (citable) accession number: Q9FZ36
Secondary accession number(s): F4HYM4, O22041, Q9C7M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.