Reviewed, UniProtKB/Swiss-Prot Q9FZ36 (M3K2_ARATH)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase 2
    EC=2.7.11.25
Alternative name(s):
    Arabidopsis NPK1-related protein kinase 2

Gene names

Name:	ANP2
Ordered Locus Names:	At1g54960
ORF Names:	T24C10.7, F14C21.49

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in roots and flowers. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAG51109.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis Inferred from genetic interaction. Source: TAIR protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Mitogen-activated protein kinase kinase kinase 2		PRO_0000086271
Regions
	Domain	68 – 330	263	Protein kinase
	Nucleotide binding	74 – 82	9	ATP By similarity
	Coiled coil	105 – 130	26	Potential
	Coiled coil	605 – 628	24	Potential
	Compositional bias	405 – 411	7	Poly-Asp
Sites
	Active site	196	1	Proton acceptor By similarity
	Binding site	97	1	ATP By similarity
Experimental info
	Sequence conflict	99	1	V → A in BAA21856. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ36-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0204655E1F9E918E	FASTA	651	71,849
	10 20 30 40 50 60 MQDLFGSVRR SLVFRSTTDD ENQENHPPPF PSLLADKITS CIRKSMVFAK SQSPPNNSTV 70 80 90 100 110 120 QIKPPIRWRK GQLIGRGAFG TVYMGMNLDS GELLAVKQVL ITSNCASKEK TQAHIQELEE 130 140 150 160 170 180 EVKLLKNLSH PNIVRYLGTV REDETLNILL EFVPGGSISS LLEKFGAFPE SVVRTYTNQL 190 200 210 220 230 240 LLGLEYLHNH AIMHRDIKGA NILVDNQGCI KLADFGASKQ VAELATISGA KSMKGTPYWM 250 260 270 280 290 300 APEVILQTGH SFSADIWSVG CTVIEMVTGK APWSQQYKEI AAIFHIGTTK SHPPIPDNIS 310 320 330 340 350 360 SDANDFLLKC LQQEPNLRPT ASELLKHPFV TGKQKESASK DLTSFMDNSC SPLPSELTNI 370 380 390 400 410 420 TSYQTSTSDD VGDICNLGSL TCTLAFPEKS IQNNSLCLKS NNGYDDDDDN DMCLIDDENF 430 440 450 460 470 480 LTYNGETGPS LDNNTDAKKS CDTMSEISDI LKCKFDENSG NGETETKVSM EVDHPSYSED 490 500 510 520 530 540 ENELTESKIK AFLDDKAAEL KKLQTPLYEE FYNGMITCSP ICMESNINNN KREEAPRGFL 550 560 570 580 590 600 KLPPKSRSPS QGHIGRSPSR ATDAACCSKS PESGNSSGAP KNSNASAGAE QESNSQSVAL 610 620 630 640 650 SEIERKWKEE LDQELERKRR EITRQAGMGS SPRDRSLSRH REKSRFASPG K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)." Nishihama R., Banno H., Kawahara E., Irie K., Machida Y. Plant J. 12:39-48(1997) [PubMed: 9263451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-651, TISSUE SPECIFICITY. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC064840 Genomic DNA. Translation: AAG00876.1. AC069144 Genomic DNA. Translation: AAG51109.1. Sequence problems. AB000798 mRNA. Translation: BAA21856.1.
IPI	IPI00523113.
PIR	A96591.
3D structure databases
SMR	Q9FZ36. Positions 68-331.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT1G54960 in contig CT485782_GR.
Organism-specific databases
GeneFarm	862. 89.
TAIR	At1g54960.
Phylogenomic databases
eggNOG	KOG0198.
HOGENOM	HBG318239.
InParanoid	Q9FZ36.
PhylomeDB	Q9FZ36.
Enzyme and pathway databases
BRENDA	2.7.11.25. 302.
Gene expression databases
ArrayExpress	Q9FZ36.
Genevestigator	Q9FZ36.
GermOnline	AT1G54960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR015748. MAPKKK3. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22986:SF64. MAPKKK3. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K2_ARATH

Accession

Primary (citable) accession number: Q9FZ36
Secondary accession number(s): O22041, Q9C7M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)