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Protein

Mitogen-activated protein kinase kinase kinase 2

Gene

ANP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei196Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 2 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related protein kinase 2
Gene namesi
Name:ANP2
Ordered Locus Names:At1g54960
ORF Names:F14C21.49, T24C10.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G54960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862711 – 651Mitogen-activated protein kinase kinase kinase 2Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9FZ36
PRIDEiQ9FZ36

PTM databases

iPTMnetiQ9FZ36

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9FZ36 baseline and differential
GenevisibleiQ9FZ36 AT

Interactioni

Protein-protein interaction databases

BioGridi27162, 1 interactor
STRINGi3702.AT1G54960.1

Structurei

3D structure databases

ProteinModelPortaliQ9FZ36
SMRiQ9FZ36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 330Protein kinasePROSITE-ProRule annotationAdd BLAST263

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili105 – 130Sequence analysisAdd BLAST26
Coiled coili605 – 628Sequence analysisAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi405 – 411Poly-Asp7

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
ENOG410XQGS LUCA
HOGENOMiHOG000033953
InParanoidiQ9FZ36
OrthoDBiEOG09360531
PhylomeDBiQ9FZ36

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FZ36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDLFGSVRR SLVFRSTTDD ENQENHPPPF PSLLADKITS CIRKSMVFAK
60 70 80 90 100
SQSPPNNSTV QIKPPIRWRK GQLIGRGAFG TVYMGMNLDS GELLAVKQVL
110 120 130 140 150
ITSNCASKEK TQAHIQELEE EVKLLKNLSH PNIVRYLGTV REDETLNILL
160 170 180 190 200
EFVPGGSISS LLEKFGAFPE SVVRTYTNQL LLGLEYLHNH AIMHRDIKGA
210 220 230 240 250
NILVDNQGCI KLADFGASKQ VAELATISGA KSMKGTPYWM APEVILQTGH
260 270 280 290 300
SFSADIWSVG CTVIEMVTGK APWSQQYKEI AAIFHIGTTK SHPPIPDNIS
310 320 330 340 350
SDANDFLLKC LQQEPNLRPT ASELLKHPFV TGKQKESASK DLTSFMDNSC
360 370 380 390 400
SPLPSELTNI TSYQTSTSDD VGDICNLGSL TCTLAFPEKS IQNNSLCLKS
410 420 430 440 450
NNGYDDDDDN DMCLIDDENF LTYNGETGPS LDNNTDAKKS CDTMSEISDI
460 470 480 490 500
LKCKFDENSG NGETETKVSM EVDHPSYSED ENELTESKIK AFLDDKAAEL
510 520 530 540 550
KKLQTPLYEE FYNGMITCSP ICMESNINNN KREEAPRGFL KLPPKSRSPS
560 570 580 590 600
QGHIGRSPSR ATDAACCSKS PESGNSSGAP KNSNASAGAE QESNSQSVAL
610 620 630 640 650
SEIERKWKEE LDQELERKRR EITRQAGMGS SPRDRSLSRH REKSRFASPG

K
Length:651
Mass (Da):71,849
Last modified:March 1, 2001 - v1
Checksum:i0204655E1F9E918E
GO

Sequence cautioni

The sequence AAG51109 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99V → A in BAA21856 (PubMed:9263451).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC064840 Genomic DNA Translation: AAG00876.1
AC069144 Genomic DNA Translation: AAG51109.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33167.2
CP002684 Genomic DNA Translation: ANM58828.1
AB000798 mRNA Translation: BAA21856.1
PIRiA96591
RefSeqiNP_001319236.1, NM_001333684.1
NP_175894.5, NM_104370.5

Genome annotation databases

EnsemblPlantsiAT1G54960.1; AT1G54960.1; AT1G54960
AT1G54960.2; AT1G54960.2; AT1G54960
GeneIDi841937
GrameneiAT1G54960.1; AT1G54960.1; AT1G54960
AT1G54960.2; AT1G54960.2; AT1G54960
KEGGiath:AT1G54960

Similar proteinsi

Entry informationi

Entry nameiM3K2_ARATH
AccessioniPrimary (citable) accession number: Q9FZ36
Secondary accession number(s): F4HYM4, O22041, Q9C7M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health