Q9FZ29 (MAN1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 1
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
Short name=AtMAN1

Gene names

Name:	MAN1
Ordered Locus Names:	At1g02310
ORF Names:	T6A9.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in roots, stems and flowers. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 411	394	Mannan endo-1,4-beta-mannosidase 1		PRO_0000277474
Sites
	Active site	198	1	Proton donor By similarity
	Active site	319	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ29 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B72ECB54C4D7F218	FASTA	411	46,290
	10 20 30 40 50 60 MLNILPFFLF FLPFLIGNNR ICVAVKTGFV GRNGTQFVLN GEQVYLNGFN AYWMMTTAAD 70 80 90 100 110 120 TASKGRATVT TALRQASAVG MNVARIWGFN EGDYIPLQIS PGSYSEDVFK GLDFVVYEAG 130 140 150 160 170 180 RFNIKLIISL VNNFEDYGGR KKYVEWAGLD EPDEFYTNSA VKQFYKNHVK TVLTRKNTIT 190 200 210 220 230 240 GRMYKDDPTI FSWELINEPR CNDSTASNIL QDWVKEMASY VKSIDSNHLL EIGLEGFYGE 250 260 270 280 290 300 SIPERTVYNP GGRVLTGTDF ITNNQIPDID FATIHIYPDS WLPLQSSRTG EQDTFVDRWI 310 320 330 340 350 360 GAHIEDCDNI IKKPLLITEF GKSSKYPGFS LEKRNKFFQR VYDVIYDSAR AGGSCTGGVF 370 380 390 400 410 WQLTTNRTGL LGDGYEVFMQ AGPNTTAQLI ADQSSKLKNL KYPPLVTHSA E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC064879 Genomic DNA. Translation: AAG00883.1. CP002684 Genomic DNA. Translation: AEE27414.1. AY081352 mRNA. Translation: AAL91241.1. BT002154 mRNA. Translation: AAN72165.1.
IPI	IPI00516697.
PIR	D86153.
RefSeq	NP_171733.2. NM_100112.3.
UniGene	At.23486. At.42610.
3D structure databases
ProteinModelPortal	Q9FZ29.
SMR	Q9FZ29. Positions 29-400.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G02310.1-P.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Proteomic databases
PaxDb	Q9FZ29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02310.1; AT1G02310.1; AT1G02310.
GeneID	839444.
KEGG	ath:AT1G02310.
Organism-specific databases
TAIR	At1g02310.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169952.
InParanoid	Q9FZ29.
OMA	KEMASYV.
PhylomeDB	Q9FZ29.
ProtClustDB	CLSN2917984.
Gene expression databases
Genevestigator	Q9FZ29.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN1_ARATH

Accession

Primary (citable) accession number: Q9FZ29

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program