Reviewed, UniProtKB/Swiss-Prot Q9FZ29 (MAN1_ARATH)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase 1
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase 1
    Endo-beta-1,4-mannanase 1
      Short name=AtMAN1

Gene names

Name:	MAN1
Ordered Locus Names:	At1g02310
ORF Names:	T6A9.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in roots, stems and flowers. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 411	394	Mannan endo-1,4-beta-mannosidase 1		PRO_0000277474
Sites
	Active site	198	1	Proton donor By similarity
	Active site	319	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	384	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ29-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B72ECB54C4D7F218	FASTA	411	46,290
	10 20 30 40 50 60 MLNILPFFLF FLPFLIGNNR ICVAVKTGFV GRNGTQFVLN GEQVYLNGFN AYWMMTTAAD 70 80 90 100 110 120 TASKGRATVT TALRQASAVG MNVARIWGFN EGDYIPLQIS PGSYSEDVFK GLDFVVYEAG 130 140 150 160 170 180 RFNIKLIISL VNNFEDYGGR KKYVEWAGLD EPDEFYTNSA VKQFYKNHVK TVLTRKNTIT 190 200 210 220 230 240 GRMYKDDPTI FSWELINEPR CNDSTASNIL QDWVKEMASY VKSIDSNHLL EIGLEGFYGE 250 260 270 280 290 300 SIPERTVYNP GGRVLTGTDF ITNNQIPDID FATIHIYPDS WLPLQSSRTG EQDTFVDRWI 310 320 330 340 350 360 GAHIEDCDNI IKKPLLITEF GKSSKYPGFS LEKRNKFFQR VYDVIYDSAR AGGSCTGGVF 370 380 390 400 410 WQLTTNRTGL LGDGYEVFMQ AGPNTTAQLI ADQSSKLKNL KYPPLVTHSA E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed: 16897088] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC064879 Genomic DNA. Translation: AAG00883.1. AY081352 mRNA. Translation: AAL91241.1. BT002154 mRNA. Translation: AAN72165.1.
IPI	IPI00516697.
PIR	D86153.
RefSeq	NP_171733.2.
UniGene	At.42610
3D structure databases
HSSP	HSSP built from PDB template 1QNR based on UniProtKB Q99036.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Proteomic databases
PRIDE	Q9FZ29.
Genome annotation databases
GeneID	839444.
GenomeReviews	Gene locus AT1G02310 in contig CT485782_GR.
KEGG	ath:AT1G02310.
NMPDR	fig|3702.1.peg.350.
Organism-specific databases
TAIR	At1g02310.
Phylogenomic databases
OMA	WIATHER.
Enzyme and pathway databases
BRENDA	3.2.1.78. 302.
Gene expression databases
ArrayExpress	Q9FZ29.
Genevestigator	Q9FZ29.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN1_ARATH

Accession

Primary (citable) accession number: Q9FZ29

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)