Reviewed, UniProtKB/Swiss-Prot Q9FZ27 (GL22_ARATH)

Names and origin

Protein names

Recommended name:
    Germin-like protein subfamily 2 member 2

Gene names

Ordered Locus Names:	At1g02335
ORF Names:	T6A9.29, T6A9.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.
Subunit structure	Oligomer (believed to be a pentamer but probably hexamer) By similarity.
Subcellular location	Secreted › extracellular space › apoplast By similarity.
Sequence similarities	Belongs to the germin family.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Ligand	Manganese Metal-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 219	197	Germin-like protein subfamily 2 member 2		PRO_0000010822
Sites
	Metal binding	109	1	Manganese By similarity
	Metal binding	111	1	Manganese By similarity
	Metal binding	116	1	Manganese By similarity
	Metal binding	155	1	Manganese By similarity
Amino acid modifications
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 47		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ27-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4D6D80460BDFDFE8	FASTA	219	23,441
	10 20 30 40 50 60 MMNSRISIII ALSCIMITSI RAYDPDALQD LCVADKSHGT KLNGFPCKET LNITESDFFF 70 80 90 100 110 120 AGISKPAVIN STMGSAVTGA NVEKIPGLNT LSVSLARIDY APGGLNPPHT HPRATEVVYV 130 140 150 160 170 180 LEGELEVGFI TTANKLFTKT IKIGEVFVFP RGLVHFQKNN GKSPASVLSA FNSQLPGTAS 190 200 210 VAATLFAAEP ALPEDVLTKT FQVGSKMVDK IKERLATKK

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC064879 Genomic DNA. Translation: AAG00885.1. AK176405 mRNA. Translation: BAD44168.1.
IPI	IPI00540989.
PIR	F86153.
RefSeq	NP_001077450.1.
UniGene	At.27586 Rra.433 Rsa.6808
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9FZ27.
Genome annotation databases
GeneID	3766642.
GenomeReviews	Gene locus AT1G02335 in contig CT485782_GR.
KEGG	ath:AT1G02335.
Organism-specific databases
TAIR	At1g02335.
Phylogenomic databases
HOGENOM	HBG744545.
Gene expression databases
Genevestigator	Q9FZ27.
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
SMART	SM00835. Cupin_1. 1 hit. [Graphical view]
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GL22_ARATH

Accession

Primary (citable) accession number: Q9FZ27
Secondary accession number(s): Q67YR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	March 1, 2001
Last modified:	December 15, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)