Q9FZ22 (GPAT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycerol-3-phosphate acyltransferase 2
Short name=AtGPAT2
EC=2.3.1.15

Gene names

Name:	GPAT2
Ordered Locus Names:	At1g02390
ORF Names:	T6A9.8, T6A9.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential. Note: Not mitochondrial.
Tissue specificity	Weakly or not expressed in roots, leaves, seedlings, developing siliques and flower buds. Ref.4
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Probable glycerol-3-phosphate acyltransferase 2		PRO_0000195250
Regions
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Transmembrane	275 – 295	21	Helical; Potential
	Motif	339 – 344	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ22 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 600A3D3243F11071	FASTA	530	59,968
	10 20 30 40 50 60 MSGNKISTLQ ALVFFLYRFF ILRRWCHRSP KQKYQKCPSH GLHQYQDLSN HTLIFNVEGA 70 80 90 100 110 120 LLKSNSLFPY FMVVAFEAGG VIRSLFLLVL YPFISLMSYE MGLKTMVMLS FFGVKKESFR 130 140 150 160 170 180 VGKSVLPKYF LEDVGLEMFQ VLKRGGKRVA VSDLPQVMID VFLRDYLEIE VVVGRDMKMV 190 200 210 220 230 240 GGYYLGIVED KKNLEIAFDK VVQEERLGSG RRLIGITSFN SPSHRSLFSQ FCQEIYFVRN 250 260 270 280 290 300 SDKKSWQTLP QDQYPKPLIF HDGRLAVKPT PLNTLVLFMW APFAAVLAAA RLVFGLNLPY 310 320 330 340 350 360 SLANPFLAFS GIHLTLTVNN HNDLISADRK RGCLFVCNHR TLLDPLYISY ALRKKNMKAV 370 380 390 400 410 420 TYSLSRLSEL LAPIKTVRLT RDRVKDGQAM EKLLSQGDLV VCPEGTTCRE PYLLRFSPLF 430 440 450 460 470 480 SEVCDVIVPV AIDSHVTFFY GTTASGLKAF DPIFFLLNPF PSYTVKLLDP VSGSSSSTCR 490 500 510 520 530 GVPDNGKVNF EVANHVQHEI GNALGFECTN LTRRDKYLIL AGNNGVVKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility." Zheng Z., Xia Q., Dauk M., Shen W., Selvaraj G., Zou J. Plant Cell 15:1872-1887(2003) [PubMed: 12897259] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC064879 Genomic DNA. Translation: AAG00890.1. CP002684 Genomic DNA. Translation: AEE27423.1. AF419560 mRNA. Translation: AAL31892.1. AY097339 mRNA. Translation: AAM19855.1.
IPI	IPI00532090.
PIR	C86154.
RefSeq	NP_563651.1. NM_100120.2.
UniGene	At.27394.
3D structure databases
ProteinModelPortal	Q9FZ22.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FZ22.
Proteomic databases
PRIDE	Q9FZ22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02390.1; AT1G02390.1; AT1G02390.
GeneID	839558.
GenomeReviews	Gene locus AT1G02390 in contig CT485782_GR.
KEGG	ath:AT1G02390.
NMPDR	fig|3702.1.peg.358.
Organism-specific databases
TAIR	At1g02390.
Phylogenomic databases
GeneTree	EPGT00050000001610.
HOGENOM	HBG590278.
InParanoid	Q9FZ22.
OMA	ANPFLAF.
PhylomeDB	Q9FZ22.
ProtClustDB	PLN02588.
Gene expression databases
Genevestigator	Q9FZ22.
GermOnline	AT1G02390. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
KO	K13508.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT2_ARATH

Accession

Primary (citable) accession number: Q9FZ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program