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Q9FZ03

- MAN2_SOLLC

UniProt

Q9FZ03 - MAN2_SOLLC

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Protein

Mannan endo-1,4-beta-mannosidase 2

Gene

MAN2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Possesses endo-beta-mannanase activity in vitro. May be involved in seed germination by weakening the endosperm cap prior to radicle emergence.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111Proton donorBy similarity
Active sitei328 – 3281NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 2 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
LeMAN2
Gene namesi
Name:MAN2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Chromosome 6

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 414389Mannan endo-1,4-beta-mannosidase 2PRO_5000057062Add
BLAST

Expressioni

Developmental stagei

Expressed specifically in the endosperm cap before radicle emergence.1 Publication

Inductioni

By gibberellin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9FZ03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FZ03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN
60 70 80 90 100
GSPFLFNGFN SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG
110 120 130 140 150
GDRALQISPG VYDERVFQGL DFVISEAKKY GIRLILSFVN NYNDFGGKAQ
160 170 180 190 200
YVQWARNAGA QINGDDDFYT NYITKNYYKN HIKKVVTRFN TITGMTYKDD
210 220 230 240 250
STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK HLLEIGMEGF
260 270 280 290 300
YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA
310 320 330 340 350
QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI
360 370 380 390 400
YRNIYSLAKD GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS
410
QSHAMTALAH LVKI
Length:414
Mass (Da):46,835
Last modified:December 4, 2007 - v2
Checksum:i2ED692448896046E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184238 mRNA. Translation: AAG00315.2.
RefSeqiNP_001234473.1. NM_001247544.1.
UniGeneiLes.108.

Genome annotation databases

EnsemblPlantsiSolyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
GeneIDi544170.
KEGGisly:544170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184238 mRNA. Translation: AAG00315.2 .
RefSeqi NP_001234473.1. NM_001247544.1.
UniGenei Les.108.

3D structure databases

ProteinModelPortali Q9FZ03.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi Solyc06g064520.2.1 ; Solyc06g064520.2.1 ; Solyc06g064520.2 .
GeneIDi 544170.
KEGGi sly:544170.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
    Nonogaki H., Gee O.H., Bradford K.J.
    Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Moneymaker.
    Tissue: Seed.
  2. Nonogaki H., Gee O.H., Bradford K.J.
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 170-174.

Entry informationi

Entry nameiMAN2_SOLLC
AccessioniPrimary (citable) accession number: Q9FZ03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3