Q9FZ03 (MAN2_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 2
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
LeMAN2

Gene names

Name:

MAN2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses endo-beta-mannanase activity in vitro. May be involved in seed germination by weakening the endosperm cap prior to radicle emergence. Ref.1
Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Developmental stage	Expressed specifically in the endosperm cap before radicle emergence. Ref.1
Induction	By gibberellin. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 414	389	Mannan endo-1,4-beta-mannosidase 2		PRO_5000057062
Sites
	Active site	211	1	Proton donor By similarity
	Active site	328	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FZ03 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 2ED692448896046E	FASTA	414	46,835
	10 20 30 40 50 60 MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN GSPFLFNGFN 70 80 90 100 110 120 SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG GDRALQISPG VYDERVFQGL 130 140 150 160 170 180 DFVISEAKKY GIRLILSFVN NYNDFGGKAQ YVQWARNAGA QINGDDDFYT NYITKNYYKN 190 200 210 220 230 240 HIKKVVTRFN TITGMTYKDD STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK 250 260 270 280 290 300 HLLEIGMEGF YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA 310 320 330 340 350 360 QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI YRNIYSLAKD 370 380 390 400 410 GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS QSHAMTALAH LVKI

References

[1]	"A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds." Nonogaki H., Gee O.H., Bradford K.J. Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Moneymaker. Tissue: Seed.
[2]	Nonogaki H., Gee O.H., Bradford K.J. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 170-174.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184238 mRNA. Translation: AAG00315.2.
RefSeq	NP_001234473.1. NM_001247544.1.
UniGene	Les.108.
3D structure databases
ProteinModelPortal	Q9FZ03.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	Solyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
GeneID	544170.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN2_SOLLC

Accession

Primary (citable) accession number: Q9FZ03

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program