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Q9FZ03

- MAN2_SOLLC

UniProt

Q9FZ03 - MAN2_SOLLC

Protein

Mannan endo-1,4-beta-mannosidase 2

Gene

MAN2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Possesses endo-beta-mannanase activity in vitro. May be involved in seed germination by weakening the endosperm cap prior to radicle emergence.1 Publication

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei211 – 2111Proton donorBy similarity
    Active sitei328 – 3281NucleophileBy similarity

    GO - Molecular functioni

    1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase 2 (EC:3.2.1.78)
    Alternative name(s):
    Beta-mannanase 2
    Endo-beta-1,4-mannanase 2
    LeMAN2
    Gene namesi
    Name:MAN2
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Chromosome 6

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 414389Mannan endo-1,4-beta-mannosidase 2PRO_5000057062Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed specifically in the endosperm cap before radicle emergence.1 Publication

    Inductioni

    By gibberellin.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FZ03.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FZ03-1 [UniParc]FASTAAdd to Basket

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    MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN    50
    GSPFLFNGFN SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG 100
    GDRALQISPG VYDERVFQGL DFVISEAKKY GIRLILSFVN NYNDFGGKAQ 150
    YVQWARNAGA QINGDDDFYT NYITKNYYKN HIKKVVTRFN TITGMTYKDD 200
    STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK HLLEIGMEGF 250
    YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA 300
    QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI 350
    YRNIYSLAKD GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS 400
    QSHAMTALAH LVKI 414
    Length:414
    Mass (Da):46,835
    Last modified:December 4, 2007 - v2
    Checksum:i2ED692448896046E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184238 mRNA. Translation: AAG00315.2.
    RefSeqiNP_001234473.1. NM_001247544.1.
    UniGeneiLes.108.

    Genome annotation databases

    EnsemblPlantsiSolyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
    GeneIDi544170.
    KEGGisly:544170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184238 mRNA. Translation: AAG00315.2 .
    RefSeqi NP_001234473.1. NM_001247544.1.
    UniGenei Les.108.

    3D structure databases

    ProteinModelPortali Q9FZ03.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Solyc06g064520.2.1 ; Solyc06g064520.2.1 ; Solyc06g064520.2 .
    GeneIDi 544170.
    KEGGi sly:544170.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
      Nonogaki H., Gee O.H., Bradford K.J.
      Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: cv. Moneymaker.
      Tissue: Seed.
    2. Nonogaki H., Gee O.H., Bradford K.J.
      Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 170-174.

    Entry informationi

    Entry nameiMAN2_SOLLC
    AccessioniPrimary (citable) accession number: Q9FZ03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3