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Q9FZ03 (MAN2_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 2

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
LeMAN2
Gene names
Name:MAN2
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possesses endo-beta-mannanase activity in vitro. May be involved in seed germination by weakening the endosperm cap prior to radicle emergence. Ref.1

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted Potential.

Developmental stage

Expressed specifically in the endosperm cap before radicle emergence. Ref.1

Induction

By gibberellin. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 414389Mannan endo-1,4-beta-mannosidase 2
PRO_5000057062

Sites

Active site2111Proton donor By similarity
Active site3281Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FZ03 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 2ED692448896046E

FASTA41446,835
        10         20         30         40         50         60 
MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN GSPFLFNGFN 

        70         80         90        100        110        120 
SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG GDRALQISPG VYDERVFQGL 

       130        140        150        160        170        180 
DFVISEAKKY GIRLILSFVN NYNDFGGKAQ YVQWARNAGA QINGDDDFYT NYITKNYYKN 

       190        200        210        220        230        240 
HIKKVVTRFN TITGMTYKDD STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK 

       250        260        270        280        290        300 
HLLEIGMEGF YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA 

       310        320        330        340        350        360 
QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI YRNIYSLAKD 

       370        380        390        400        410 
GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS QSHAMTALAH LVKI 

« Hide

References

[1]"A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
Nonogaki H., Gee O.H., Bradford K.J.
Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
Strain: cv. Moneymaker.
Tissue: Seed.
[2]Nonogaki H., Gee O.H., Bradford K.J.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 170-174.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184238 mRNA. Translation: AAG00315.2.
RefSeqNP_001234473.1. NM_001247544.1.
UniGeneLes.108.

3D structure databases

ProteinModelPortalQ9FZ03.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
GeneID544170.
KEGGsly:544170.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAN2_SOLLC
AccessionPrimary (citable) accession number: Q9FZ03
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 4, 2007
Last modified: February 19, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries