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Q9FYT6 (CRS1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Alternative name(s):
Chloroplastic RNA splicing factor 1
Protein CHLOROPLAST RNA SPLICING 1
Gene names
Name:CRS1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the splicing of group IIA introns in chloroplasts, and especially for atpF, by regulating the intron folding. Forms splicing particles with RNA. Also involved in chloroplast protein translation. Ref.1 Ref.2 Ref.3

Subunit structure

Homodimer. Interacts with RNA, specifically with atpF intron. Part of large ribonucleo-protein complexes that include group IIA introns and CRS1. Ref.1 Ref.3

Subcellular location

Plastidchloroplast stroma Ref.1.

Tissue specificity

More expressed in leaves than in roots. Ref.1

Sequence similarities

Contains 3 CRM domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 7-7.5.

Temperature dependence:

Optimum temperature is 25-30 degrees Celsius.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
Translation regulation
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
Transit peptide
   LigandRNA-binding
   Molecular functionRibonucleoprotein
Gene Ontology (GO)
   Biological_processGroup II intron splicing

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of translation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast stroma

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribonucleoprotein complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FYT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Chloroplast Potential
Chain41 – 715675Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
PRO_0000283625

Regions

Domain168 – 26598CRM 1
Domain350 – 44798CRM 2
Domain562 – 662101CRM 3
Coiled coil448 – 47326 Potential
Coiled coil502 – 55049 Potential
Compositional bias3 – 5755Pro-rich

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 9ADC6ECE35F6C55F

FASTA71581,406
        10         20         30         40         50         60 
MAPPPLPLFS PSLKAPPPPP WLHGSSTQSR DSAPPVPPLP AEATPSKFRI DSPKPAPARK 

        70         80         90        100        110        120 
NTKTAAKPLT AGVPGGRTHR AVLGIIRRVR SLELSDAPSP NSVHTSNSGA AAAAFHLTIE 

       130        140        150        160        170        180 
LSPPREPGQY VVEKEKSRAV PWAAARDEGL KVALRREKKP REPTRAETEL ETHELRRLRR 

       190        200        210        220        230        240 
LARGIGRWAR AKKAGVTDEV VKEVRREWAS GEELAAVRIV EPLRRSMDRA REILEIKTGG 

       250        260        270        280        290        300 
LVVWTKGDMH FVYRGSKYQQ NAKHSHTFLT NVHKDDAFQE NDQSICGQKD EEPVKGTLYE 

       310        320        330        340        350        360 
REVNRLLDTL GPRFVDWWWD TPLPVDADLL PEFVPGSKTP YRLCPPGVRP TLADEELTYL 

       370        380        390        400        410        420 
RKLARLLPTH FALGRNTRLQ GLAAAILKLW EKSLIAKIAV KIGIQNTNNE QMAWNLKHLT 

       430        440        450        460        470        480 
GGTVILRNKD FIILYRGKDF LPGGVAQTVI QREAQVHDEQ VKEEEARLKA VDSLQMVGEL 

       490        500        510        520        530        540 
SEESSLGTFR EYQGFHAKFV HENTENSNTM IELEAEKYRL EKELKDHEWK LSVLNKKIER 

       550        560        570        580        590        600 
SNQALAKLHS SWSPSEQSAD REHLTEEEKI MFRRIGRKMD GLVLLGRRGI FDGVIEEIHQ 

       610        620        630        640        650        660 
HWKHKEVVKV ITKQNQTRQI MYAASLLEVE TGGILIAVEK LTTSHAIILY RGKNYRRPAK 

       670        680        690        700        710 
SSFSNLLTKR EALRRSIEVQ RRGSMKYFVR ERQKSILELK RKLRYVTRQI RYRTP 

« Hide

References

[1]"CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin."
Till B., Schmitz-Linneweber C., Williams-Carrier R., Barkan A.
RNA 7:1227-1238(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH RNA.
[2]"Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants."
Vogel J., Boerner T., Hess W.R.
Nucleic Acids Res. 27:3866-3874(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains."
Ostersetzer O., Cooke A.M., Watkins K.P., Barkan A.
Plant Cell 17:241-255(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, HOMODIMER, INTERACTION WITH ATPF INTRON.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF290414 mRNA. Translation: AAG00595.1.
RefSeqNP_001105008.1. NM_001111538.1. [Q9FYT6-1]
UniGeneZm.661.

3D structure databases

ProteinModelPortalQ9FYT6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48743N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID541870.
KEGGzma:541870.

Organism-specific databases

GrameneQ9FYT6.
MaizeGDB106357.

Phylogenomic databases

HOGENOMHOG000241574.

Family and domain databases

Gene3D3.30.110.60. 3 hits.
InterProIPR001890. RNA-binding_CRM.
[Graphical view]
PfamPF01985. CRS1_YhbY. 3 hits.
[Graphical view]
SMARTSM01103. CRS1_YhbY. 3 hits.
[Graphical view]
SUPFAMSSF75471. SSF75471. 3 hits.
PROSITEPS51295. CRM. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRS1_MAIZE
AccessionPrimary (citable) accession number: Q9FYT6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families