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Q9FYT6

- CRS1_MAIZE

UniProt

Q9FYT6 - CRS1_MAIZE

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Protein
Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Gene
CRS1
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the splicing of group IIA introns in chloroplasts, and especially for atpF, by regulating the intron folding. Forms splicing particles with RNA. Also involved in chloroplast protein translation.3 Publications

pH dependencei

Optimum pH is 7-7.5.

Temperature dependencei

Optimum temperature is 25-30 degrees Celsius.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. Group II intron splicing Source: EnsemblPlants/Gramene
  2. mRNA processing Source: UniProtKB-KW
  3. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Alternative name(s):
Chloroplastic RNA splicing factor 1
Protein CHLOROPLAST RNA SPLICING 1
Gene namesi
Name:CRS1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ9FYT6.
MaizeGDBi106357.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
  2. ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040Chloroplast Reviewed prediction
Add
BLAST
Chaini41 – 715675Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
PRO_0000283625Add
BLAST

Expressioni

Tissue specificityi

More expressed in leaves than in roots.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with RNA, specifically with atpF intron. Part of large ribonucleo-protein complexes that include group IIA introns and CRS1.2 Publications

Protein-protein interaction databases

DIPiDIP-48743N.

Structurei

3D structure databases

ProteinModelPortaliQ9FYT6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 26598CRM 1
Add
BLAST
Domaini350 – 44798CRM 2
Add
BLAST
Domaini562 – 662101CRM 3
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili448 – 47326 Reviewed prediction
Add
BLAST
Coiled coili502 – 55049 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 5755Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 3 CRM domains.

Keywords - Domaini

Coiled coil, Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000241574.

Family and domain databases

Gene3Di3.30.110.60. 3 hits.
InterProiIPR001890. RNA-binding_CRM.
[Graphical view]
PfamiPF01985. CRS1_YhbY. 3 hits.
[Graphical view]
SMARTiSM01103. CRS1_YhbY. 3 hits.
[Graphical view]
SUPFAMiSSF75471. SSF75471. 3 hits.
PROSITEiPS51295. CRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FYT6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAPPPLPLFS PSLKAPPPPP WLHGSSTQSR DSAPPVPPLP AEATPSKFRI    50
DSPKPAPARK NTKTAAKPLT AGVPGGRTHR AVLGIIRRVR SLELSDAPSP 100
NSVHTSNSGA AAAAFHLTIE LSPPREPGQY VVEKEKSRAV PWAAARDEGL 150
KVALRREKKP REPTRAETEL ETHELRRLRR LARGIGRWAR AKKAGVTDEV 200
VKEVRREWAS GEELAAVRIV EPLRRSMDRA REILEIKTGG LVVWTKGDMH 250
FVYRGSKYQQ NAKHSHTFLT NVHKDDAFQE NDQSICGQKD EEPVKGTLYE 300
REVNRLLDTL GPRFVDWWWD TPLPVDADLL PEFVPGSKTP YRLCPPGVRP 350
TLADEELTYL RKLARLLPTH FALGRNTRLQ GLAAAILKLW EKSLIAKIAV 400
KIGIQNTNNE QMAWNLKHLT GGTVILRNKD FIILYRGKDF LPGGVAQTVI 450
QREAQVHDEQ VKEEEARLKA VDSLQMVGEL SEESSLGTFR EYQGFHAKFV 500
HENTENSNTM IELEAEKYRL EKELKDHEWK LSVLNKKIER SNQALAKLHS 550
SWSPSEQSAD REHLTEEEKI MFRRIGRKMD GLVLLGRRGI FDGVIEEIHQ 600
HWKHKEVVKV ITKQNQTRQI MYAASLLEVE TGGILIAVEK LTTSHAIILY 650
RGKNYRRPAK SSFSNLLTKR EALRRSIEVQ RRGSMKYFVR ERQKSILELK 700
RKLRYVTRQI RYRTP 715
Length:715
Mass (Da):81,406
Last modified:March 1, 2001 - v1
Checksum:i9ADC6ECE35F6C55F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF290414 mRNA. Translation: AAG00595.1.
RefSeqiNP_001105008.1. NM_001111538.1. [Q9FYT6-1]
UniGeneiZm.661.

Genome annotation databases

GeneIDi541870.
KEGGizma:541870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF290414 mRNA. Translation: AAG00595.1 .
RefSeqi NP_001105008.1. NM_001111538.1. [Q9FYT6-1 ]
UniGenei Zm.661.

3D structure databases

ProteinModelPortali Q9FYT6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48743N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 541870.
KEGGi zma:541870.

Organism-specific databases

Gramenei Q9FYT6.
MaizeGDBi 106357.

Phylogenomic databases

HOGENOMi HOG000241574.

Family and domain databases

Gene3Di 3.30.110.60. 3 hits.
InterProi IPR001890. RNA-binding_CRM.
[Graphical view ]
Pfami PF01985. CRS1_YhbY. 3 hits.
[Graphical view ]
SMARTi SM01103. CRS1_YhbY. 3 hits.
[Graphical view ]
SUPFAMi SSF75471. SSF75471. 3 hits.
PROSITEi PS51295. CRM. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin."
    Till B., Schmitz-Linneweber C., Williams-Carrier R., Barkan A.
    RNA 7:1227-1238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH RNA.
  2. "Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants."
    Vogel J., Boerner T., Hess W.R.
    Nucleic Acids Res. 27:3866-3874(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains."
    Ostersetzer O., Cooke A.M., Watkins K.P., Barkan A.
    Plant Cell 17:241-255(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HOMODIMER, INTERACTION WITH ATPF INTRON.

Entry informationi

Entry nameiCRS1_MAIZE
AccessioniPrimary (citable) accession number: Q9FYT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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