Reviewed, UniProtKB/Swiss-Prot Q9FYT6 (CRS1_MAIZE)

Names and origin

Protein names

Recommended name:
    Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Alternative name(s):
    Protein CHLOROPLAST RNA SPLICING 1
    Chloroplastic RNA splicing factor 1

Gene names

Name:

CRS1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the splicing of group IIA introns in chloroplasts, and especially for atpF, by regulating the intron folding. Forms splicing particles with RNA. Also involved in chloroplast protein translation. Ref.1 Ref.2 Ref.3
Subunit structure	Homodimer. Interacts with RNA, specifically with atpF intron. Part of large ribonucleo-protein complexes that include group IIA introns and CRS1. Ref.1 Ref.3
Subcellular location	Plastid › chloroplast stroma Ref.1.
Tissue specificity	More expressed in leaves than in roots. Ref.1
Sequence similarities	Contains 3 CRM domains.
Biophysicochemical properties	pH dependence: Optimum pH is 7-7.5. Temperature dependence: Optimum temperature is 25-30 degrees Celsius.

Ontologies

Keywords
Biological process	Translation regulation mRNA processing mRNA splicing
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Transit peptide
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9FYT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Potential
	Chain	41 – 715	675	Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic		PRO_0000283625
Regions
	Domain	168 – 265	98	CRM 1
	Domain	350 – 447	98	CRM 2
	Domain	562 – 662	101	CRM 3
	Coiled coil	448 – 473	26	Potential
	Coiled coil	502 – 550	49	Potential
	Compositional bias	3 – 57	55	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9ADC6ECE35F6C55F	FASTA	715	81,406
	10 20 30 40 50 60 MAPPPLPLFS PSLKAPPPPP WLHGSSTQSR DSAPPVPPLP AEATPSKFRI DSPKPAPARK 70 80 90 100 110 120 NTKTAAKPLT AGVPGGRTHR AVLGIIRRVR SLELSDAPSP NSVHTSNSGA AAAAFHLTIE 130 140 150 160 170 180 LSPPREPGQY VVEKEKSRAV PWAAARDEGL KVALRREKKP REPTRAETEL ETHELRRLRR 190 200 210 220 230 240 LARGIGRWAR AKKAGVTDEV VKEVRREWAS GEELAAVRIV EPLRRSMDRA REILEIKTGG 250 260 270 280 290 300 LVVWTKGDMH FVYRGSKYQQ NAKHSHTFLT NVHKDDAFQE NDQSICGQKD EEPVKGTLYE 310 320 330 340 350 360 REVNRLLDTL GPRFVDWWWD TPLPVDADLL PEFVPGSKTP YRLCPPGVRP TLADEELTYL 370 380 390 400 410 420 RKLARLLPTH FALGRNTRLQ GLAAAILKLW EKSLIAKIAV KIGIQNTNNE QMAWNLKHLT 430 440 450 460 470 480 GGTVILRNKD FIILYRGKDF LPGGVAQTVI QREAQVHDEQ VKEEEARLKA VDSLQMVGEL 490 500 510 520 530 540 SEESSLGTFR EYQGFHAKFV HENTENSNTM IELEAEKYRL EKELKDHEWK LSVLNKKIER 550 560 570 580 590 600 SNQALAKLHS SWSPSEQSAD REHLTEEEKI MFRRIGRKMD GLVLLGRRGI FDGVIEEIHQ 610 620 630 640 650 660 HWKHKEVVKV ITKQNQTRQI MYAASLLEVE TGGILIAVEK LTTSHAIILY RGKNYRRPAK 670 680 690 700 710 SSFSNLLTKR EALRRSIEVQ RRGSMKYFVR ERQKSILELK RKLRYVTRQI RYRTP

References

[1]	"CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin." Till B., Schmitz-Linneweber C., Williams-Carrier R., Barkan A. RNA 7:1227-1238(2001) [PubMed: 11565746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH RNA.
[2]	"Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants." Vogel J., Boerner T., Hess W.R. Nucleic Acids Res. 27:3866-3874(1999) [PubMed: 10481026] [Abstract] Cited for: FUNCTION.
[3]	"CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains." Ostersetzer O., Cooke A.M., Watkins K.P., Barkan A. Plant Cell 17:241-255(2005) [PubMed: 15598799] [Abstract] Cited for: FUNCTION, HOMODIMER, INTERACTION WITH ATPF INTRON.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF290414 mRNA. Translation: AAG00595.1.
RefSeq	NP_001105008.1.
UniGene	Zm.661
3D structure databases
SMR	Q9FYT6. Positions 168-257, 564-653.
ModBase	Search...
Genome annotation databases
GeneID	541870.
KEGG	zma:541870.
Organism-specific databases
Gramene	Q9FYT6.
MaizeGDB	106357.
Family and domain databases
InterPro	IPR001890. RNA-binding_CRM. [Graphical view]
Gene3D	G3DSA:3.30.110.60. CRS1_YhbY. 2 hits.
Pfam	PF01985. CRS1_YhbY. 3 hits. [Graphical view]
PROSITE	PS51295. CRM. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRS1_MAIZE

Accession

Primary (citable) accession number: Q9FYT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)