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Q9FYT6

- CRS1_MAIZE

UniProt

Q9FYT6 - CRS1_MAIZE

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Protein

Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic

Gene

CRS1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Required for the splicing of group IIA introns in chloroplasts, and especially for atpF, by regulating the intron folding. Forms splicing particles with RNA. Also involved in chloroplast protein translation.3 Publications

pH dependencei

Optimum pH is 7-7.5.

Temperature dependencei

Optimum temperature is 25-30 degrees Celsius.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. Group II intron splicing Source: EnsemblPlants/Gramene
  2. mRNA processing Source: UniProtKB-KW
  3. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
Alternative name(s):
Chloroplastic RNA splicing factor 1
Protein CHLOROPLAST RNA SPLICING 1
Gene namesi
Name:CRS1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ9FYT6.
MaizeGDBi106357.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
  2. ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040ChloroplastSequence AnalysisAdd
BLAST
Chaini41 – 715675Chloroplastic group IIA intron splicing facilitator CRS1, chloroplasticPRO_0000283625Add
BLAST

Expressioni

Tissue specificityi

More expressed in leaves than in roots.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with RNA, specifically with atpF intron. Part of large ribonucleo-protein complexes that include group IIA introns and CRS1.2 Publications

Protein-protein interaction databases

DIPiDIP-48743N.

Structurei

3D structure databases

ProteinModelPortaliQ9FYT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 26598CRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini350 – 44798CRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini562 – 662101CRM 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili448 – 47326Sequence AnalysisAdd
BLAST
Coiled coili502 – 55049Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 5755Pro-richAdd
BLAST

Sequence similaritiesi

Contains 3 CRM domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000241574.

Family and domain databases

Gene3Di3.30.110.60. 3 hits.
InterProiIPR001890. RNA-binding_CRM.
[Graphical view]
PfamiPF01985. CRS1_YhbY. 3 hits.
[Graphical view]
SMARTiSM01103. CRS1_YhbY. 3 hits.
[Graphical view]
SUPFAMiSSF75471. SSF75471. 3 hits.
PROSITEiPS51295. CRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FYT6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPPLPLFS PSLKAPPPPP WLHGSSTQSR DSAPPVPPLP AEATPSKFRI
60 70 80 90 100
DSPKPAPARK NTKTAAKPLT AGVPGGRTHR AVLGIIRRVR SLELSDAPSP
110 120 130 140 150
NSVHTSNSGA AAAAFHLTIE LSPPREPGQY VVEKEKSRAV PWAAARDEGL
160 170 180 190 200
KVALRREKKP REPTRAETEL ETHELRRLRR LARGIGRWAR AKKAGVTDEV
210 220 230 240 250
VKEVRREWAS GEELAAVRIV EPLRRSMDRA REILEIKTGG LVVWTKGDMH
260 270 280 290 300
FVYRGSKYQQ NAKHSHTFLT NVHKDDAFQE NDQSICGQKD EEPVKGTLYE
310 320 330 340 350
REVNRLLDTL GPRFVDWWWD TPLPVDADLL PEFVPGSKTP YRLCPPGVRP
360 370 380 390 400
TLADEELTYL RKLARLLPTH FALGRNTRLQ GLAAAILKLW EKSLIAKIAV
410 420 430 440 450
KIGIQNTNNE QMAWNLKHLT GGTVILRNKD FIILYRGKDF LPGGVAQTVI
460 470 480 490 500
QREAQVHDEQ VKEEEARLKA VDSLQMVGEL SEESSLGTFR EYQGFHAKFV
510 520 530 540 550
HENTENSNTM IELEAEKYRL EKELKDHEWK LSVLNKKIER SNQALAKLHS
560 570 580 590 600
SWSPSEQSAD REHLTEEEKI MFRRIGRKMD GLVLLGRRGI FDGVIEEIHQ
610 620 630 640 650
HWKHKEVVKV ITKQNQTRQI MYAASLLEVE TGGILIAVEK LTTSHAIILY
660 670 680 690 700
RGKNYRRPAK SSFSNLLTKR EALRRSIEVQ RRGSMKYFVR ERQKSILELK
710
RKLRYVTRQI RYRTP
Length:715
Mass (Da):81,406
Last modified:March 1, 2001 - v1
Checksum:i9ADC6ECE35F6C55F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290414 mRNA. Translation: AAG00595.1.
RefSeqiNP_001105008.1. NM_001111538.1. [Q9FYT6-1]
UniGeneiZm.661.

Genome annotation databases

GeneIDi541870.
KEGGizma:541870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290414 mRNA. Translation: AAG00595.1 .
RefSeqi NP_001105008.1. NM_001111538.1. [Q9FYT6-1 ]
UniGenei Zm.661.

3D structure databases

ProteinModelPortali Q9FYT6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48743N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 541870.
KEGGi zma:541870.

Organism-specific databases

Gramenei Q9FYT6.
MaizeGDBi 106357.

Phylogenomic databases

HOGENOMi HOG000241574.

Family and domain databases

Gene3Di 3.30.110.60. 3 hits.
InterProi IPR001890. RNA-binding_CRM.
[Graphical view ]
Pfami PF01985. CRS1_YhbY. 3 hits.
[Graphical view ]
SMARTi SM01103. CRS1_YhbY. 3 hits.
[Graphical view ]
SUPFAMi SSF75471. SSF75471. 3 hits.
PROSITEi PS51295. CRM. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin."
    Till B., Schmitz-Linneweber C., Williams-Carrier R., Barkan A.
    RNA 7:1227-1238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH RNA.
  2. "Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants."
    Vogel J., Boerner T., Hess W.R.
    Nucleic Acids Res. 27:3866-3874(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains."
    Ostersetzer O., Cooke A.M., Watkins K.P., Barkan A.
    Plant Cell 17:241-255(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HOMODIMER, INTERACTION WITH ATPF INTRON.

Entry informationi

Entry nameiCRS1_MAIZE
AccessioniPrimary (citable) accession number: Q9FYT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3