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Q9FYT6

- CRS1_MAIZE

UniProt

Q9FYT6 - CRS1_MAIZE

Protein

Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic

Gene

CRS1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Required for the splicing of group IIA introns in chloroplasts, and especially for atpF, by regulating the intron folding. Forms splicing particles with RNA. Also involved in chloroplast protein translation.3 Publications

    pH dependencei

    Optimum pH is 7-7.5.

    Temperature dependencei

    Optimum temperature is 25-30 degrees Celsius.

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. Group II intron splicing Source: EnsemblPlants/Gramene
    2. mRNA processing Source: UniProtKB-KW
    3. regulation of translation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
    Alternative name(s):
    Chloroplastic RNA splicing factor 1
    Protein CHLOROPLAST RNA SPLICING 1
    Gene namesi
    Name:CRS1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ9FYT6.
    MaizeGDBi106357.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell
    2. ribonucleoprotein complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4040ChloroplastSequence AnalysisAdd
    BLAST
    Chaini41 – 715675Chloroplastic group IIA intron splicing facilitator CRS1, chloroplasticPRO_0000283625Add
    BLAST

    Expressioni

    Tissue specificityi

    More expressed in leaves than in roots.1 Publication

    Interactioni

    Subunit structurei

    Homodimer. Interacts with RNA, specifically with atpF intron. Part of large ribonucleo-protein complexes that include group IIA introns and CRS1.2 Publications

    Protein-protein interaction databases

    DIPiDIP-48743N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FYT6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 26598CRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini350 – 44798CRM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini562 – 662101CRM 3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili448 – 47326Sequence AnalysisAdd
    BLAST
    Coiled coili502 – 55049Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 5755Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 CRM domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000241574.

    Family and domain databases

    Gene3Di3.30.110.60. 3 hits.
    InterProiIPR001890. RNA-binding_CRM.
    [Graphical view]
    PfamiPF01985. CRS1_YhbY. 3 hits.
    [Graphical view]
    SMARTiSM01103. CRS1_YhbY. 3 hits.
    [Graphical view]
    SUPFAMiSSF75471. SSF75471. 3 hits.
    PROSITEiPS51295. CRM. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FYT6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPPPLPLFS PSLKAPPPPP WLHGSSTQSR DSAPPVPPLP AEATPSKFRI    50
    DSPKPAPARK NTKTAAKPLT AGVPGGRTHR AVLGIIRRVR SLELSDAPSP 100
    NSVHTSNSGA AAAAFHLTIE LSPPREPGQY VVEKEKSRAV PWAAARDEGL 150
    KVALRREKKP REPTRAETEL ETHELRRLRR LARGIGRWAR AKKAGVTDEV 200
    VKEVRREWAS GEELAAVRIV EPLRRSMDRA REILEIKTGG LVVWTKGDMH 250
    FVYRGSKYQQ NAKHSHTFLT NVHKDDAFQE NDQSICGQKD EEPVKGTLYE 300
    REVNRLLDTL GPRFVDWWWD TPLPVDADLL PEFVPGSKTP YRLCPPGVRP 350
    TLADEELTYL RKLARLLPTH FALGRNTRLQ GLAAAILKLW EKSLIAKIAV 400
    KIGIQNTNNE QMAWNLKHLT GGTVILRNKD FIILYRGKDF LPGGVAQTVI 450
    QREAQVHDEQ VKEEEARLKA VDSLQMVGEL SEESSLGTFR EYQGFHAKFV 500
    HENTENSNTM IELEAEKYRL EKELKDHEWK LSVLNKKIER SNQALAKLHS 550
    SWSPSEQSAD REHLTEEEKI MFRRIGRKMD GLVLLGRRGI FDGVIEEIHQ 600
    HWKHKEVVKV ITKQNQTRQI MYAASLLEVE TGGILIAVEK LTTSHAIILY 650
    RGKNYRRPAK SSFSNLLTKR EALRRSIEVQ RRGSMKYFVR ERQKSILELK 700
    RKLRYVTRQI RYRTP 715
    Length:715
    Mass (Da):81,406
    Last modified:March 1, 2001 - v1
    Checksum:i9ADC6ECE35F6C55F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290414 mRNA. Translation: AAG00595.1.
    RefSeqiNP_001105008.1. NM_001111538.1. [Q9FYT6-1]
    UniGeneiZm.661.

    Genome annotation databases

    GeneIDi541870.
    KEGGizma:541870.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290414 mRNA. Translation: AAG00595.1 .
    RefSeqi NP_001105008.1. NM_001111538.1. [Q9FYT6-1 ]
    UniGenei Zm.661.

    3D structure databases

    ProteinModelPortali Q9FYT6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48743N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 541870.
    KEGGi zma:541870.

    Organism-specific databases

    Gramenei Q9FYT6.
    MaizeGDBi 106357.

    Phylogenomic databases

    HOGENOMi HOG000241574.

    Family and domain databases

    Gene3Di 3.30.110.60. 3 hits.
    InterProi IPR001890. RNA-binding_CRM.
    [Graphical view ]
    Pfami PF01985. CRS1_YhbY. 3 hits.
    [Graphical view ]
    SMARTi SM01103. CRS1_YhbY. 3 hits.
    [Graphical view ]
    SUPFAMi SSF75471. SSF75471. 3 hits.
    PROSITEi PS51295. CRM. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin."
      Till B., Schmitz-Linneweber C., Williams-Carrier R., Barkan A.
      RNA 7:1227-1238(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, INTERACTION WITH RNA.
    2. "Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants."
      Vogel J., Boerner T., Hess W.R.
      Nucleic Acids Res. 27:3866-3874(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains."
      Ostersetzer O., Cooke A.M., Watkins K.P., Barkan A.
      Plant Cell 17:241-255(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, HOMODIMER, INTERACTION WITH ATPF INTRON.

    Entry informationi

    Entry nameiCRS1_MAIZE
    AccessioniPrimary (citable) accession number: Q9FYT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3