Reviewed, UniProtKB/Swiss-Prot Q9FYP9 (IMA2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Importin subunit alpha-2

Gene names

Ordered Locus Names:	Os01g0158000, LOC_Os01g06470
ORF Names:	P0011G08.44

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Ref.3
Subunit structure	Forms a complex with importin subunit beta-1. The whole complex, most stable and composed of importin alpha, importin beta and NLS substrate, is referred to as PTAC or pore targeting complex.
Subcellular location	Cytoplasm › perinuclear region.
Tissue specificity	Expressed in root, callus, and etiolated leaf. Low expression in green leaf. Ref.3 Ref.1
Induction	By dark in green leaf. Down-regulated by light. Ref.3
Sequence similarities	Belongs to the importin alpha family. Contains 8 ARM repeats.
Sequence caution	The sequence BAB08186.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB64664.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Importin subunit alpha-2		PRO_0000120742
Regions
	Repeat	125 – 165	41	ARM 1
	Repeat	167 – 206	40	ARM 2
	Repeat	209 – 248	40	ARM 3
	Repeat	253 – 292	40	ARM 4
	Repeat	294 – 335	42	ARM 5
	Repeat	338 – 383	46	ARM 6
	Repeat	386 – 425	40	ARM 7
	Repeat	438 – 477	40	ARM 8
Experimental info
	Sequence conflict	231	1	S → N in BAA31220. Ref.1
	Sequence conflict	349	1	H → N in BAA31220. Ref.1
	Sequence conflict	408	1	M → A in BAA31220. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FYP9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0B7885BBE87F55B8	FASTA	528	57,186
	10 20 30 40 50 60 MADDSASPSP SSASPLQHHR EALKSSVRNT AASRRREQAI AIGKERREAL IRAKRVCRAP 70 80 90 100 110 120 ISGSDEAEME EGDMVVDEEK ACLEAKTAHA VEELKSALSI QGKGVQKKKI EALRDLRRLL 130 140 150 160 170 180 SQPEVPLVDT AIKAGAVPLL VQYLSFGSSD EQLLEAAWCL TNIAAGEPEE TKSLLPALPL 190 200 210 220 230 240 LIAHLGEKSS TLVAEQCAWA IGNVAGEGAE LRSTLLAQGA LRPLTRLMFS SKGSTARTAA 250 260 270 280 290 300 WAMSNLIKGP DPKAANELIT IDGVLNAIIA SLEKEDEELA TEVAWVVVYL SALSDRGISL 310 320 330 340 350 360 IVRSSVPQLL IGRLFSSENL QLLIPVLRGL GNLIAADDYM VDSVLTVGHN IIDQALSGLI 370 380 390 400 410 420 KCLKSDNRVL RKESSWALSN IAAGSFEHKK LIFASEATPV LIRLVTSMQF DIRREAAYTL 430 440 450 460 470 480 GNLCVVPTGN CELPKIIVEH LVAIVDGGAL PGFIHLVRSA DVDTAGLGLQ FLELVMRGYP 490 500 510 520 NKQGPKLVEM EDGIEAMERF QFHENEQMRN MANGLVDEYF GEDYGLDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel importin alpha from rice, a component involved in the process of nuclear protein transport." Iwasaki T., Matsuki R., Shoji K., Sanmiya K., Miyao M., Yamamoto N. FEBS Lett. 428:259-262(1998) [PubMed: 9654145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: cv. Nipponbare.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported." Jiang C.-J., Shoji K., Matsuki R., Baba A., Inagaki N., Ban H., Iwasaki T., Imamoto N., Yoneda Y., Deng X.-W., Yamamoto N. J. Biol. Chem. 276:9322-9329(2001) [PubMed: 11124253] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78504 mRNA. Translation: BAA31220.1. AB006788 Genomic DNA. Translation: BAA31222.1. AP002539 Genomic DNA. Translation: BAB08186.1. Sequence problems. AP003225 Genomic DNA. Translation: BAB64664.1. Sequence problems.
PIR	T02972.
RefSeq	NP_001042074.1.
UniGene	Os.68623
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324088.
KEGG	osa:4324088.
NMPDR	fig|39947.1.peg.1287.
Organism-specific databases
Gramene	Q9FYP9.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF00514. Arm. 5 hits. [Graphical view]
SMART	SM00185. ARM. 6 hits. [Graphical view]
PROSITE	PS50176. ARM_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMA2_ORYSJ

Accession

Primary (citable) accession number: Q9FYP9
Secondary accession number(s): O80332

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2001
Last modified:	November 24, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)