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Reviewed, UniProtKB/Swiss-Prot Q9FY99 (G6PD2_ARATH)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic
      Short name=G6PDH2
      Short name=G6PD2
    EC=1.1.1.49
Gene names
Ordered Locus Names: At5g13110
ORF Names: T19L5_70
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activity

D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast.

Developmental stage

Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence caution

The sequence CAC05439.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

pentose-phosphate shunt, oxidative branch

Inferred from direct assay. Source: TAIR

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from direct assay. Source: TAIR

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Potential
Chain51 – 596546Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic
PRO_0000010436

Sites

Active site3451Proton acceptor By similarity
Binding site1181NADP By similarity
Binding site1501NADP By similarity
Binding site2831Substrate By similarity
Binding site2871Substrate By similarity

Amino acid modifications

Disulfide bond168 ↔ 176Redox modulation By similarity

Experimental info

Sequence conflict1451I → V in CAA59011. Ref.3
Sequence conflict1901H → I in CAA59011. Ref.3
Sequence conflict2011T → I in CAA59011. Ref.3
Sequence conflict4891L → F in CAA59011. Ref.3
Sequence conflict5421D → G in AAL57678. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q9FY99-1 [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: BDD89BEB49EF6E3B

FASTA59667,160
        10         20         30         40         50         60 
MAALSSSVTT RSYHSGYLAS FSPVNGDRHR SLSFLSASPQ GLNPLDLCVR FQRKSGRASV 

        70         80         90        100        110        120 
FMQDGAIVTN SNSSESKTSL KGLKDEVLSA LSQEAAKVGV ESDGQSQSTV SITVVGASGD 

       130        140        150        160        170        180 
LAKKKIFPAL FALYYEGCLP EHFTIFGYSR SKMTDVELRN MVSKTLTCRI DKRANCGEKM 

       190        200        210        220        230        240 
EEFLKRCFYH SGQYDSQEHF TELDKKLKEH EAGRISNRLF YLSIPPNIFV DAVKCASTSA 

       250        260        270        280        290        300 
SSVNGWTRVI VEKPFGRDSE TSAALTKSLK QYLEEDQIFR IDHYLGKELV ENLSVLRFSN 

       310        320        330        340        350        360 
LIFEPLWSRQ YIRNVQFIFS EDFGTEGRGG YFDNYGIIRD IMQNHLLQIL ALFAMETPVS 

       370        380        390        400        410        420 
LDAEDIRNEK VKVLRSMRPI RVEDVVIGQY KSHTKGGVTY PAYTDDKTVP KGSLTPTFAA 

       430        440        450        460        470        480 
AALFIDNARW DGVPFLMKAG KALHTRSAEI RVQFRHVPGN LYNRNTGSDL DQATNELVIR 

       490        500        510        520        530        540 
VQPDEAIYLK INNKVPGLGM RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR 

       550        560        570        580        590 
SDELDAAWSL FTPLLKEIEE KKRIPEYYPY GSRGPVGAHY LAAKHKVQWG DVSIDQ

« Hide

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References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana."
Fink A., Greppin H., Tacchini P.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-596.
Strain: cv. Columbia.

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Cross-references

Sequence databases

AL391711 Genomic DNA. Translation: CAC05439.1. Sequence problems.
AY065042 mRNA. Translation: AAL57678.1.
X84229 mRNA. Translation: CAA59011.1.
IPIIPI00531306.
PIRS71245.
RefSeqNP_196815.2.
UniGeneAt.61

3D structure databases

HSSPHSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBaseSearch...

Proteomic databases

PRIDEQ9FY99.

Genome annotation databases

GeneID831150.
GenomeReviewsGene locus AT5G13110 in contig BA000015_GR.
KEGGath:AT5G13110.
NMPDRfig|3702.1.peg.23426.

Organism-specific databases

TAIRAt5g13110.

Phylogenomic databases

OMAQ9FY99. NYPAGSW.

Enzyme and pathway databases

BRENDA1.1.1.49. 302.

Gene expression databases

ArrayExpressQ9FY99.
GermOnlineAT5G13110. Arabidopsis thaliana.

Family and domain databases

InterProIPR001282. Glc-6-P_DH.
IPR019796. Glc-6-P_DH_AS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23429. G6PDH. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
ProDomPD001129. G6PD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameG6PD2_ARATH
AccessionPrimary (citable) accession number: Q9FY99
Secondary accession number(s): Q43728, Q8VZD6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents