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Q9FY99

- G6PD2_ARATH

UniProt

Q9FY99 - G6PD2_ARATH

Protein

Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic

Gene

At5g13110

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (25 Nov 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei150 – 1501NADPBy similarity
    Binding sitei253 – 2531NADP; via carbonyl oxygenBy similarity
    Binding sitei253 – 2531SubstrateBy similarity
    Binding sitei321 – 3211SubstrateBy similarity
    Binding sitei340 – 3401SubstrateBy similarity
    Active sitei345 – 3451Proton acceptorBy similarity
    Binding sitei441 – 4411SubstrateBy similarity
    Binding sitei446 – 4461SubstrateBy similarity
    Binding sitei482 – 4821SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi116 – 1238NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: TAIR
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glucose metabolic process Source: TAIR
    2. pentose-phosphate shunt, oxidative branch Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (EC:1.1.1.49)
    Short name:
    G6PD2
    Short name:
    G6PDH2
    Gene namesi
    Ordered Locus Names:At5g13110
    ORF Names:T19L5_70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G13110.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050ChloroplastSequence AnalysisAdd
    BLAST
    Chaini51 – 596546Glucose-6-phosphate 1-dehydrogenase 2, chloroplasticPRO_0000010436Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi168 ↔ 176Redox modulationBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ9FY99.
    PRIDEiQ9FY99.

    Expressioni

    Developmental stagei

    Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.

    Gene expression databases

    GenevestigatoriQ9FY99.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi16428. 2 interactions.
    IntActiQ9FY99. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FY99.
    SMRiQ9FY99. Positions 110-575.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni283 – 2875Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    InParanoidiQ9FY99.
    KOiK00036.
    OMAiWRWVEPL.
    PhylomeDBiQ9FY99.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FY99-1 [UniParc]FASTAAdd to Basket

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    MAALSSSVTT RSYHSGYLAS FSPVNGDRHR SLSFLSASPQ GLNPLDLCVR    50
    FQRKSGRASV FMQDGAIVTN SNSSESKTSL KGLKDEVLSA LSQEAAKVGV 100
    ESDGQSQSTV SITVVGASGD LAKKKIFPAL FALYYEGCLP EHFTIFGYSR 150
    SKMTDVELRN MVSKTLTCRI DKRANCGEKM EEFLKRCFYH SGQYDSQEHF 200
    TELDKKLKEH EAGRISNRLF YLSIPPNIFV DAVKCASTSA SSVNGWTRVI 250
    VEKPFGRDSE TSAALTKSLK QYLEEDQIFR IDHYLGKELV ENLSVLRFSN 300
    LIFEPLWSRQ YIRNVQFIFS EDFGTEGRGG YFDNYGIIRD IMQNHLLQIL 350
    ALFAMETPVS LDAEDIRNEK VKVLRSMRPI RVEDVVIGQY KSHTKGGVTY 400
    PAYTDDKTVP KGSLTPTFAA AALFIDNARW DGVPFLMKAG KALHTRSAEI 450
    RVQFRHVPGN LYNRNTGSDL DQATNELVIR VQPDEAIYLK INNKVPGLGM 500
    RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR SDELDAAWSL 550
    FTPLLKEIEE KKRIPEYYPY GSRGPVGAHY LAAKHKVQWG DVSIDQ 596
    Length:596
    Mass (Da):67,160
    Last modified:November 25, 2002 - v2
    Checksum:iBDD89BEB49EF6E3B
    GO

    Sequence cautioni

    The sequence CAC05439.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451I → V in CAA59011. 1 PublicationCurated
    Sequence conflicti190 – 1901H → I in CAA59011. 1 PublicationCurated
    Sequence conflicti201 – 2011T → I in CAA59011. 1 PublicationCurated
    Sequence conflicti489 – 4891L → F in CAA59011. 1 PublicationCurated
    Sequence conflicti542 – 5421D → G in AAL57678. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL391711 Genomic DNA. Translation: CAC05439.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED91851.1.
    AY065042 mRNA. Translation: AAL57678.1.
    X84229 mRNA. Translation: CAA59011.1.
    PIRiS71245.
    RefSeqiNP_196815.2. NM_121314.3.
    UniGeneiAt.61.

    Genome annotation databases

    EnsemblPlantsiAT5G13110.1; AT5G13110.1; AT5G13110.
    GeneIDi831150.
    KEGGiath:AT5G13110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL391711 Genomic DNA. Translation: CAC05439.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED91851.1 .
    AY065042 mRNA. Translation: AAL57678.1 .
    X84229 mRNA. Translation: CAA59011.1 .
    PIRi S71245.
    RefSeqi NP_196815.2. NM_121314.3.
    UniGenei At.61.

    3D structure databases

    ProteinModelPortali Q9FY99.
    SMRi Q9FY99. Positions 110-575.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16428. 2 interactions.
    IntActi Q9FY99. 1 interaction.

    Proteomic databases

    PaxDbi Q9FY99.
    PRIDEi Q9FY99.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G13110.1 ; AT5G13110.1 ; AT5G13110 .
    GeneIDi 831150.
    KEGGi ath:AT5G13110.

    Organism-specific databases

    TAIRi AT5G13110.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    InParanoidi Q9FY99.
    KOi K00036.
    OMAi WRWVEPL.
    PhylomeDBi Q9FY99.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Gene expression databases

    Genevestigatori Q9FY99.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana."
      Fink A., Greppin H., Tacchini P.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-596.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PD2_ARATH
    AccessioniPrimary (citable) accession number: Q9FY99
    Secondary accession number(s): Q43728, Q8VZD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: November 25, 2002
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3