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Q9FY99 (G6PD2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic

Short name=G6PD2
Short name=G6PDH2
EC=1.1.1.49
Gene names
Ordered Locus Names:At5g13110
ORF Names:T19L5_70
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00966

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00966.

Developmental stage

Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth. HAMAP-Rule MF_00966

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence caution

The sequence CAC05439.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Potential
Chain51 – 596546Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic HAMAP-Rule MF_00966
PRO_0000010436

Regions

Nucleotide binding116 – 1238NADP By similarity
Region283 – 2875Substrate binding By similarity

Sites

Active site3451Proton acceptor By similarity
Binding site1501NADP By similarity
Binding site2531NADP; via carbonyl oxygen By similarity
Binding site2531Substrate By similarity
Binding site3211Substrate By similarity
Binding site3401Substrate By similarity
Binding site4411Substrate By similarity
Binding site4461Substrate By similarity
Binding site4821Substrate By similarity

Amino acid modifications

Disulfide bond168 ↔ 176Redox modulation By similarity

Experimental info

Sequence conflict1451I → V in CAA59011. Ref.4
Sequence conflict1901H → I in CAA59011. Ref.4
Sequence conflict2011T → I in CAA59011. Ref.4
Sequence conflict4891L → F in CAA59011. Ref.4
Sequence conflict5421D → G in AAL57678. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9FY99 [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: BDD89BEB49EF6E3B

FASTA59667,160
        10         20         30         40         50         60 
MAALSSSVTT RSYHSGYLAS FSPVNGDRHR SLSFLSASPQ GLNPLDLCVR FQRKSGRASV 

        70         80         90        100        110        120 
FMQDGAIVTN SNSSESKTSL KGLKDEVLSA LSQEAAKVGV ESDGQSQSTV SITVVGASGD 

       130        140        150        160        170        180 
LAKKKIFPAL FALYYEGCLP EHFTIFGYSR SKMTDVELRN MVSKTLTCRI DKRANCGEKM 

       190        200        210        220        230        240 
EEFLKRCFYH SGQYDSQEHF TELDKKLKEH EAGRISNRLF YLSIPPNIFV DAVKCASTSA 

       250        260        270        280        290        300 
SSVNGWTRVI VEKPFGRDSE TSAALTKSLK QYLEEDQIFR IDHYLGKELV ENLSVLRFSN 

       310        320        330        340        350        360 
LIFEPLWSRQ YIRNVQFIFS EDFGTEGRGG YFDNYGIIRD IMQNHLLQIL ALFAMETPVS 

       370        380        390        400        410        420 
LDAEDIRNEK VKVLRSMRPI RVEDVVIGQY KSHTKGGVTY PAYTDDKTVP KGSLTPTFAA 

       430        440        450        460        470        480 
AALFIDNARW DGVPFLMKAG KALHTRSAEI RVQFRHVPGN LYNRNTGSDL DQATNELVIR 

       490        500        510        520        530        540 
VQPDEAIYLK INNKVPGLGM RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR 

       550        560        570        580        590 
SDELDAAWSL FTPLLKEIEE KKRIPEYYPY GSRGPVGAHY LAAKHKVQWG DVSIDQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana."
Fink A., Greppin H., Tacchini P.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-596.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL391711 Genomic DNA. Translation: CAC05439.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91851.1.
AY065042 mRNA. Translation: AAL57678.1.
X84229 mRNA. Translation: CAA59011.1.
PIRS71245.
RefSeqNP_196815.2. NM_121314.3.
UniGeneAt.61.

3D structure databases

ProteinModelPortalQ9FY99.
SMRQ9FY99. Positions 110-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid16428. 2 interactions.
IntActQ9FY99. 1 interaction.

Proteomic databases

PaxDbQ9FY99.
PRIDEQ9FY99.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G13110.1; AT5G13110.1; AT5G13110.
GeneID831150.
KEGGath:AT5G13110.

Organism-specific databases

TAIRAT5G13110.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
InParanoidQ9FY99.
KOK00036.
OMAWRWVEPL.
PhylomeDBQ9FY99.

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Gene expression databases

GenevestigatorQ9FY99.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD2_ARATH
AccessionPrimary (citable) accession number: Q9FY99
Secondary accession number(s): Q43728, Q8VZD6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: May 14, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names