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Q9FY99

- G6PD2_ARATH

UniProt

Q9FY99 - G6PD2_ARATH

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Protein

Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic

Gene

At5g13110

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei150 – 1501NADPBy similarity
Binding sitei253 – 2531NADP; via carbonyl oxygenBy similarity
Binding sitei253 – 2531SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei340 – 3401SubstrateBy similarity
Active sitei345 – 3451Proton acceptorBy similarity
Binding sitei441 – 4411SubstrateBy similarity
Binding sitei446 – 4461SubstrateBy similarity
Binding sitei482 – 4821SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1238NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: TAIR
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glucose metabolic process Source: TAIR
  2. pentose-phosphate shunt, oxidative branch Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (EC:1.1.1.49)
Short name:
G6PD2
Short name:
G6PDH2
Gene namesi
Ordered Locus Names:At5g13110
ORF Names:T19L5_70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G13110.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050ChloroplastSequence AnalysisAdd
BLAST
Chaini51 – 596546Glucose-6-phosphate 1-dehydrogenase 2, chloroplasticPRO_0000010436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi168 ↔ 176Redox modulationBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9FY99.
PRIDEiQ9FY99.

Expressioni

Developmental stagei

Increase of activity in the apex linked to the early stages of the transition from vegetative to reproductive growth.

Gene expression databases

GenevestigatoriQ9FY99.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi16428. 2 interactions.
IntActiQ9FY99. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FY99.
SMRiQ9FY99. Positions 110-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni283 – 2875Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046192.
InParanoidiQ9FY99.
KOiK00036.
OMAiWRWVEPL.
PhylomeDBiQ9FY99.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FY99-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALSSSVTT RSYHSGYLAS FSPVNGDRHR SLSFLSASPQ GLNPLDLCVR
60 70 80 90 100
FQRKSGRASV FMQDGAIVTN SNSSESKTSL KGLKDEVLSA LSQEAAKVGV
110 120 130 140 150
ESDGQSQSTV SITVVGASGD LAKKKIFPAL FALYYEGCLP EHFTIFGYSR
160 170 180 190 200
SKMTDVELRN MVSKTLTCRI DKRANCGEKM EEFLKRCFYH SGQYDSQEHF
210 220 230 240 250
TELDKKLKEH EAGRISNRLF YLSIPPNIFV DAVKCASTSA SSVNGWTRVI
260 270 280 290 300
VEKPFGRDSE TSAALTKSLK QYLEEDQIFR IDHYLGKELV ENLSVLRFSN
310 320 330 340 350
LIFEPLWSRQ YIRNVQFIFS EDFGTEGRGG YFDNYGIIRD IMQNHLLQIL
360 370 380 390 400
ALFAMETPVS LDAEDIRNEK VKVLRSMRPI RVEDVVIGQY KSHTKGGVTY
410 420 430 440 450
PAYTDDKTVP KGSLTPTFAA AALFIDNARW DGVPFLMKAG KALHTRSAEI
460 470 480 490 500
RVQFRHVPGN LYNRNTGSDL DQATNELVIR VQPDEAIYLK INNKVPGLGM
510 520 530 540 550
RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR SDELDAAWSL
560 570 580 590
FTPLLKEIEE KKRIPEYYPY GSRGPVGAHY LAAKHKVQWG DVSIDQ
Length:596
Mass (Da):67,160
Last modified:November 25, 2002 - v2
Checksum:iBDD89BEB49EF6E3B
GO

Sequence cautioni

The sequence CAC05439.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451I → V in CAA59011. 1 PublicationCurated
Sequence conflicti190 – 1901H → I in CAA59011. 1 PublicationCurated
Sequence conflicti201 – 2011T → I in CAA59011. 1 PublicationCurated
Sequence conflicti489 – 4891L → F in CAA59011. 1 PublicationCurated
Sequence conflicti542 – 5421D → G in AAL57678. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391711 Genomic DNA. Translation: CAC05439.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91851.1.
AY065042 mRNA. Translation: AAL57678.1.
X84229 mRNA. Translation: CAA59011.1.
PIRiS71245.
RefSeqiNP_196815.2. NM_121314.3.
UniGeneiAt.61.

Genome annotation databases

EnsemblPlantsiAT5G13110.1; AT5G13110.1; AT5G13110.
GeneIDi831150.
KEGGiath:AT5G13110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391711 Genomic DNA. Translation: CAC05439.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED91851.1 .
AY065042 mRNA. Translation: AAL57678.1 .
X84229 mRNA. Translation: CAA59011.1 .
PIRi S71245.
RefSeqi NP_196815.2. NM_121314.3.
UniGenei At.61.

3D structure databases

ProteinModelPortali Q9FY99.
SMRi Q9FY99. Positions 110-575.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16428. 2 interactions.
IntActi Q9FY99. 1 interaction.

Proteomic databases

PaxDbi Q9FY99.
PRIDEi Q9FY99.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G13110.1 ; AT5G13110.1 ; AT5G13110 .
GeneIDi 831150.
KEGGi ath:AT5G13110.

Organism-specific databases

TAIRi AT5G13110.

Phylogenomic databases

eggNOGi COG0364.
HOGENOMi HOG000046192.
InParanoidi Q9FY99.
KOi K00036.
OMAi WRWVEPL.
PhylomeDBi Q9FY99.

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .

Gene expression databases

Genevestigatori Q9FY99.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Nucleotide sequence of a cDNA encoding the glucose-6-phosphate dehydrogenase from Arabidopsis thaliana."
    Fink A., Greppin H., Tacchini P.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-596.
    Strain: cv. Columbia.

Entry informationi

Entry nameiG6PD2_ARATH
AccessioniPrimary (citable) accession number: Q9FY99
Secondary accession number(s): Q43728, Q8VZD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: October 1, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3