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Protein

Probable sucrose-phosphate synthase 2

Gene

SPS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation. Required for nectar secretion.2 Publications

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase 3 (SPS3), Probable sucrose-phosphate synthase 4 (SPS4), Sucrose-phosphate synthase 1 (SPS1), Probable sucrose-phosphate synthase 2 (SPS2)
  2. Probable sucrose-phosphatase 3b (SPP3B), Probable sucrose-phosphatase 1 (SPP1), Probable sucrose-phosphatase 2 (SPP2), Probable sucrose-phosphatase 3a (SPP3A)
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • nectar secretion Source: UniProtKB
  • pollen wall assembly Source: UniProtKB
  • sucrose biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545

Protein family/group databases

CAZyiGT4 Glycosyltransferase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sucrose-phosphate synthase 2 (EC:2.4.1.14)
Alternative name(s):
Protein KAONASHI 2
Sucrose-phosphate synthase 2F
Short name:
AtSPS2F
Sucrose-phosphate synthase 5.2
Short name:
AtSPS5.2
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS2
Synonyms:KNS2, SPS1, SPSA2
Ordered Locus Names:At5g11110
ORF Names:T5K6.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G11110
TAIRilocus:2184891 AT5G11110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Loss of nectar secretion accompanied by starch accumulation in nectaries.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi596P → L in kns2-1; pollen grain with collapsed exine structure. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004136381 – 1047Probable sucrose-phosphate synthase 2Add BLAST1047

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei127PhosphoserineBy similarity1
Modified residuei131PhosphoserineBy similarity1
Modified residuei159PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FY54
PRIDEiQ9FY54

PTM databases

iPTMnetiQ9FY54
SwissPalmiQ9FY54

Expressioni

Tissue specificityi

Expressed in roots, cauline leaves, flower buds, flowers and anthers. Highly expressed in maturing nectaries.2 Publications

Gene expression databases

ExpressionAtlasiQ9FY54 baseline and differential
GenevisibleiQ9FY54 AT

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G11110.1

Structurei

3D structure databases

ProteinModelPortaliQ9FY54
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG0853 Eukaryota
COG0438 LUCA
HOGENOMiHOG000009685
InParanoidiQ9FY54
KOiK00696
OMAiKWQRVEF
OrthoDBiEOG093600RM
PhylomeDBiQ9FY54

Family and domain databases

CDDicd16419 HAD_SPS, 1 hit
InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR036412 HAD-like_sf
IPR006380 SPP_N
IPR035659 SPS_C
IPR000368 Sucrose_synth
IPR012819 SucrsPsyn_pln
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF05116 S6PP, 1 hit
PF00862 Sucrose_synth, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02468 sucrsPsyn_pln, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FY54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGNDWVNSY LEAILAAEPG IANSKPPGTG DSKSSLLLRE RGHFSPTRYF
60 70 80 90 100
VEEVITGFDE TDLHRSWVQA AATRSPQERN TRLENLCWRI WNLARQKKQV
110 120 130 140 150
EGKNAKREAK REREREKARR EVTAEMSEDF SEGEKADLPG EIPTPSDNNT
160 170 180 190 200
KGRMSRISSV DVFENWFAQH KEKKLYIVLI SLHGLIRGEN MELGRDSDTG
210 220 230 240 250
GQVKYVVELA RALGSMPGVY RVDLLTRQVT APDVDSSYSE PSEMLNPIDT
260 270 280 290 300
DIEQENGESS GAYIIRIPFG PKDKYVPKEL LWPHIPEFVD RALSHIMQIS
310 320 330 340 350
KVLGEQIGGG QQVWPVSIHG HYADAGDSTA LLSGALNVPM VFTGHSLGRD
360 370 380 390 400
KLEQLLKQGR PKEEINSNYK IWRRIEAEEL CLDASEIVIT STRQEVDEQW
410 420 430 440 450
RLYDGFDPVL ERKLRARMKR GVSCLGRFMP RMVVIPPGME FHHIVPHDVD
460 470 480 490 500
ADGDDENPQT ADPPIWSEIM RFFSNPRKPM ILALARPDPK KNLVTLVKAF
510 520 530 540 550
GECRPLRELA NLTLIMGNRN DIDELSSTNS SVLLSILKLI DKYDLYGQVA
560 570 580 590 600
MPKHHQQSDV PEIYRLAAKT KGVFINPAFI EPFGLTLIEA GAHGLPTVAT
610 620 630 640 650
INGGPVDIHR VLDNGLLVDP HDQQAIADAL LKLVSDRQLW GRCRQNGLNN
660 670 680 690 700
IHLFSWPEHC KTYLARIASC KQRHPKWQRV EFENSDSDSP SDSLRDINDI
710 720 730 740 750
SLNLKLSLDG EKSGSNNGVD TNLDAEDRAA ERKAEVEKAV STLAQKSKPT
760 770 780 790 800
EKFDSKMPTL KRRKNIFVIS VDCSATSDLL AVVKTVIDAA GRGSSTGFIL
810 820 830 840 850
STSMTISETH TALLSGGLKP QDFDAVICSS GSELYFTSSG SEDKTALPYT
860 870 880 890 900
LDADYHSHIE FRWGGESLRK TLIRWISSVE EKKKTKKGEI LVEDESSSTN
910 920 930 940 950
YCLSFKVKDP ALMPPMKELR KLMRNQALRC NAVYCQNGAR LNVIPVLASR
960 970 980 990 1000
SQALRYLLVR WGIDLSNMVV FVGDSGDTDY EGLLGGIHKT VILKGLASDL
1010 1020 1030 1040
REQPGNRSYP MEDVTPLNSP NITEAKECGR DAIKVALEKL GISLLKP
Length:1,047
Mass (Da):116,966
Last modified:March 1, 2001 - v1
Checksum:i0BA67F6E8DD9FA80
GO

Sequence cautioni

The sequence AAL47425 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391222 Genomic DNA Translation: CAC03459.1
CP002688 Genomic DNA Translation: AED91636.1
AY069868 mRNA Translation: AAL47425.1 Different initiation.
BT002697 mRNA Translation: AAO11613.1
PIRiT51800
RefSeqiNP_196672.3, NM_121149.4
UniGeneiAt.28444

Genome annotation databases

EnsemblPlantsiAT5G11110.1; AT5G11110.1; AT5G11110
GeneIDi830979
GrameneiAT5G11110.1; AT5G11110.1; AT5G11110
KEGGiath:AT5G11110

Similar proteinsi

Entry informationi

Entry nameiSPSA2_ARATH
AccessioniPrimary (citable) accession number: Q9FY54
Secondary accession number(s): Q8VYW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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