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Protein

E3 ubiquitin-protein ligase KEG

Gene

KEG

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates E2-dependent protein ubiquitination. Acts as a negative regulator of abscisic acid signaling. Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.3 Publications

Caution

The protein kinase domain is predicted to be catalytically inactive but PubMed:17194765 shows an in vitro activity.Curated

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei176ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 56RING-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi147 – 155ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein self-association Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • defense response Source: TAIR
  • developmental growth Source: TAIR
  • endosomal transport Source: TAIR
  • late endosome to vacuole transport Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  • protein ubiquitination Source: TAIR
  • response to abscisic acid Source: TAIR
  • secretion by cell Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAbscisic acid signaling pathway, Ubl conjugation pathway
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase KEG (EC:2.3.2.27, EC:2.7.11.1)
Alternative name(s):
Protein KEEP ON GOING
RING finger protein KEG
RING-type E3 ubiquitin transferase KEGCurated
Gene namesi
Name:KEG
Ordered Locus Names:At5g13530
ORF Names:T6I14.60, T6I14.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G13530
TAIRilocus:2185061 AT5G13530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Plants are seedling lethal and are hypersensitive to glucose and abscisic acid. High accumulation of ABI5.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29 – 31CGH → AGA: Small sterile plants unable to polyubiquitinate ABI5. 1 Publication3
Mutagenesisi176K → R: Loss of kinase activity associated with the loss of ABA-induced KEG autoubiquitination and subsequent degradation. 1 Publication1
Mutagenesisi1144G → S in keg-4/supp69; confers resistance to 6% glucose and suppresses abscisic acid signaling. Suppression of EDR1 disruption- (edr1-) mediated disease resistance. Reduced endosomal localization but increased localization to the endoplasmic reticulum and cytosol. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003561721 – 1625E3 ubiquitin-protein ligase KEGAdd BLAST1625

Post-translational modificationi

Autophosphotylated and autoubiquitinated in vitro.2 Publications
Phosphorylation enhances self-ubiquitination.2 Publications
Autoubiquitinated in response to abscisic acid (ABA) and subsequently targeted to proteolysis.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9FY48

PTM databases

iPTMnetiQ9FY48

Expressioni

Tissue specificityi

Expressed in all tissues of young seedlings. In flowering plants, only detected in the youngest part of the stem, anthers and the receptacle of immature siliques. Not found in mature leave, older parts of the stem, flower parts other than anthers or mature siliques.1 Publication

Developmental stagei

Expressed mainly in the actively growing and dividing cells.1 Publication

Gene expression databases

ExpressionAtlasiQ9FY48 baseline and differential
GenevisibleiQ9FY48 AT

Interactioni

Subunit structurei

Interacts with ABI5 and EDR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI5Q9SJN02EBI-1955729,EBI-1778690

GO - Molecular functioni

  • protein self-association Source: TAIR

Protein-protein interaction databases

BioGridi1647510 interactors.
IntActiQ9FY48 1 interactor.
STRINGi3702.AT5G13530.1

Structurei

3D structure databases

ProteinModelPortaliQ9FY48
SMRiQ9FY48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 427Protein kinasePROSITE-ProRule annotationAdd BLAST287
Repeati467 – 496ANK 1Add BLAST30
Repeati510 – 540ANK 2Add BLAST31
Repeati544 – 573ANK 3Add BLAST30
Repeati579 – 608ANK 4Add BLAST30
Repeati612 – 641ANK 5Add BLAST30
Repeati647 – 676ANK 6Add BLAST30
Repeati685 – 720ANK 7Add BLAST36
Repeati725 – 754ANK 8Add BLAST30
Repeati758 – 787ANK 9Add BLAST30
Repeati791 – 826ANK 10Add BLAST36
Repeati832 – 863ANK 11Add BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 107Asp-richAdd BLAST20
Compositional biasi1494 – 1497Poly-Glu4

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 56RING-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
KOG0504 Eukaryota
KOG4185 Eukaryota
COG0666 LUCA
HOGENOMiHOG000084006
InParanoidiQ9FY48
KOiK16279
OMAiGCRSMGI
OrthoDBiEOG0936005Q
PhylomeDBiQ9FY48

Family and domain databases

CDDicd00204 ANK, 3 hits
Gene3Di1.25.40.202 hits
3.30.40.101 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF00069 Pkinase, 1 hit
PF14634 zf-RING_5, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 9 hits
SM00184 RING, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FY48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGRVKVPCC SVCHTRYNED ERVPLLLQCG HGFCKDCLSK MFSTSSDTTL
60 70 80 90 100
TCPRCRHVSV VGNSVQGLRK NYAMLALIHA ASGGANFDCD YTDDEDDDDE
110 120 130 140 150
EDGSDEDGAR AARGFHASSS INSLCGPVIE VGAHPEMKLV RQIGEESSSG
160 170 180 190 200
GFGGVEMWDA TVAGGGGRCK HRVAVKKMTL TEDMDVEWMQ GQLESLRRAS
210 220 230 240 250
MWCRNVCTFH GVVKMDGSLC LLMDRCFGSV QSEMQRNEGR LTLEQILRYG
260 270 280 290 300
ADVARGVAEL HAAGVICMNI KPSNLLLDAS GNAVVSDYGL APILKKPTCQ
310 320 330 340 350
KTRPEFDSSK VTLYTDCVTL SPHYTAPEAW GPVKKLFWED ASGVSPESDA
360 370 380 390 400
WSFGCTLVEM CTGSTPWDGL SREEIFQAVV KARKVPPQYE RIVGVGIPRE
410 420 430 440 450
LWKMIGECLQ FKPSKRPTFN AMLATFLRHL QEIPRSPSAS PDNGIAKICE
460 470 480 490 500
VNIVQAPRAT NIGVFQDNPN NLHRVVLEGD FEGVRNILAK AAAGGGGSSV
510 520 530 540 550
RSLLEAQNAD GQSALHLACR RGSAELVEAI LEYGEANVDI VDKDGDPPLV
560 570 580 590 600
FALAAGSPQC VHVLIKKGAN VRSRLREGSG PSVAHVCSYH GQPDCMRELL
610 620 630 640 650
VAGADPNAVD DEGETVLHRA VAKKYTDCAI VILENGGSRS MTVSNAKCLT
660 670 680 690 700
PLHMCVATWN VAVIKRWVEV SSPEEISQAI NIPSPVGTAL CMAASIRKDH
710 720 730 740 750
EKEGRELVQI LLAAGADPTA QDAQHGRTAL HTAAMANNVE LVRVILDAGV
760 770 780 790 800
NANIRNVHNT IPLHMALARG ANSCVSLLLE SGSDCNIQDD EGDNAFHIAA
810 820 830 840 850
DAAKMIRENL DWLIVMLRSP DAAVDVRNHS GKTVRDFLEA LPREWISEDL
860 870 880 890 900
MEALLKRGVH LSPTIYEVGD WVKFKRGITT PLHGWQGAKP KSVGFVQTIL
910 920 930 940 950
EKEDMIIAFC SGEARVLANE VVKLIPLDRG QHVRLRADVK EPRFGWRGQS
960 970 980 990 1000
RDSVGTVLCV DEDGILRVGF PGASRGWKAD PAEMERVEEF KVGDWVRIRQ
1010 1020 1030 1040 1050
NLTSAKHGFG SVVPGSMGIV YCVRPDSSLL VELSYLPNPW HCEPEEVEPV
1060 1070 1080 1090 1100
APFRIGDRVC VKRSVAEPRY AWGGETHHSV GKISEIENDG LLIIEIPNRP
1110 1120 1130 1140 1150
IPWQADPSDM EKIDDFKVGD WVRVKASVSS PKYGWEDITR NSIGVMHSLD
1160 1170 1180 1190 1200
EDGDVGIAFC FRSKPFSCSV TDVEKVTPFH VGQEIHMTPS ITQPRLGWSN
1210 1220 1230 1240 1250
ETPATIGKVM RIDMDGTLSA QVTGRQTLWR VSPGDAELLS GFEVGDWVRS
1260 1270 1280 1290 1300
KPSLGNRPSY DWSNVGRESI AVVHSIQETG YLELACCFRK GRWSTHYTDL
1310 1320 1330 1340 1350
EKIPALKVGQ FVHFQKGITE PRWGWRAAKP DSRGIITTVH ADGEVRVAFF
1360 1370 1380 1390 1400
GLPGLWRGDP ADLEVEPMFE VGEWVRLREG VSCWKSVGPG SVGVVHGVGY
1410 1420 1430 1440 1450
EGDEWDGTTS VSFCGEQERW AGPTSHLEKA KKLVVGQKTR VKLAVKQPRF
1460 1470 1480 1490 1500
GWSGHSHGSV GTISAIDADG KLRIYTPAGS KTWMLDPSEV ETIEEEELKI
1510 1520 1530 1540 1550
GDWVRVKASI TTPTYQWGEV NPSSTGVVHR MEDGDLCVSF CFLDRLWLCK
1560 1570 1580 1590 1600
AGELERIRPF RIGDRVKIKD GLVTPRWGWG METHASKGHV VGVDANGKLR
1610 1620
IKFLWREGRP WIGDPADIVL DETSG
Length:1,625
Mass (Da):178,216
Last modified:December 16, 2008 - v2
Checksum:i8F16ECA3DC32B9C4
GO

Sequence cautioni

The sequence CAC05430 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAC05431 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ315360 mRNA Translation: ABC46683.1
AL391710 Genomic DNA Translation: CAC05430.1 Sequence problems.
AL391710 Genomic DNA Translation: CAC05431.1 Sequence problems.
CP002688 Genomic DNA Translation: AED91908.1
RefSeqiNP_196857.2, NM_121356.3 [Q9FY48-1]
UniGeneiAt.32056

Genome annotation databases

EnsemblPlantsiAT5G13530.1; AT5G13530.1; AT5G13530 [Q9FY48-1]
GeneIDi831197
GrameneiAT5G13530.1; AT5G13530.1; AT5G13530 [Q9FY48-1]
KEGGiath:AT5G13530

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKEG_ARATH
AccessioniPrimary (citable) accession number: Q9FY48
Secondary accession number(s): Q27YP2, Q9FY47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: April 25, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome