Reviewed, UniProtKB/Swiss-Prot Q9FY19 (PGLR2_JUNAS)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase
    Major pollen allergen Jun a 2
    Allergen=Jun a 2

Gene names

Name:

JNA2

Organism

Juniperus ashei (Ozark white cedar)

Taxonomic identifier

13101 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Cupressaceae › Juniperus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Disease	Allergen
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 54	34			PRO_0000024819
	Chain	55 – 507	453	Polygalacturonase		PRO_0000024820
Regions
	Repeat	215 – 241	27	PbH1 1
	Repeat	242 – 263	22	PbH1 2
	Repeat	265 – 285	21	PbH1 3
	Repeat	295 – 316	22	PbH1 4
	Repeat	324 – 345	22	PbH1 5
	Repeat	358 – 385	28	PbH1 6
Sites
	Active site	256	1	Proton donor By similarity
	Active site	279	1	By similarity
Amino acid modifications
	Glycosylation	267	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FY19-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2B2E0AA5E958FE5A	FASTA	507	55,731
	10 20 30 40 50 60 MSMKFMAALA FLALQLIVMA AGEDQSAQIM LDSDTKQYHR SSRNLRKRVH HARHDVAIVF 70 80 90 100 110 120 NVEHYGAVGD GKHDSTDAFE KTWNAACNKL SAVFLVPANK KFVVNNLVFY GPCQPHFSFK 130 140 150 160 170 180 VDGTIAAYPD PAKWKNSKIW MHFARLTDFN LMGTGVIDGQ GNRWWSDQCK TINGRTVCND 190 200 210 220 230 240 KGRPTAIKID FSKSVTVKEL TLTNSPEFHL VFGECDGVKI QGIKIKAPRD SPNTDGIDIF 250 260 270 280 290 300 ASKRFEIEKC TIGTGDDCVA VGTGSSNITI KDLTCGPGHG MSIGSLGKGN SRSEVSFVHL 310 320 330 340 350 360 DGAKFIDTQN GLRIKTWQGG SGLASHITYE NVEMINAENP ILINQFYCTS AAACKNQRSA 370 380 390 400 410 420 VKIQDVTFKN IHGTSATTAA IQLMCSDSVP CSNIKLSNVF LKLTSGKVAT CVNKNANGYY 430 440 450 460 470 480 TNPLNPSCKS LHPGRTPKEL ELHQKPTTLL MDEKMGASLN SSPPNCKNKC KGCQPCKPKL 490 500 IIVHPNQPED YYPQRWVCSC HNKIYNP

References

[1]

"Purification, Identification and cDNA cloning of Jun a 2, the second major allergen of mountain cedar pollen." Yokoyama M., Miyahara M., Shimizu K., Kino K., Tsunoo H. Biochem. Biophys. Res. Commun. 275:195-202(2000) [PubMed: 10944464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 55-63. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ404653 mRNA. Translation: CAC05582.1.
PIR	JC7366.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR2_JUNAS

Accession

Primary (citable) accession number: Q9FY19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	March 1, 2001
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)