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Protein

Polygalacturonase

Gene

JNA2

Organism
Juniperus ashei (Ozark white cedar)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei256 – 2561Proton donorPROSITE-ProRule annotation
Active sitei279 – 2791PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Major pollen allergen Jun a 2
Pectinase
Allergen: Jun a 2
Gene namesi
Name:JNA2
OrganismiJuniperus ashei (Ozark white cedar)
Taxonomic identifieri13101 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeJuniperus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3339. Jun a 2.0101.
428. Jun a 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 54341 PublicationPRO_0000024819Add
BLAST
Chaini55 – 507453PolygalacturonasePRO_0000024820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ9FY19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati215 – 24127PbH1 1Add
BLAST
Repeati242 – 26322PbH1 2Add
BLAST
Repeati265 – 28521PbH1 3Add
BLAST
Repeati295 – 31622PbH1 4Add
BLAST
Repeati324 – 34522PbH1 5Add
BLAST
Repeati358 – 38528PbH1 6Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FY19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMKFMAALA FLALQLIVMA AGEDQSAQIM LDSDTKQYHR SSRNLRKRVH
60 70 80 90 100
HARHDVAIVF NVEHYGAVGD GKHDSTDAFE KTWNAACNKL SAVFLVPANK
110 120 130 140 150
KFVVNNLVFY GPCQPHFSFK VDGTIAAYPD PAKWKNSKIW MHFARLTDFN
160 170 180 190 200
LMGTGVIDGQ GNRWWSDQCK TINGRTVCND KGRPTAIKID FSKSVTVKEL
210 220 230 240 250
TLTNSPEFHL VFGECDGVKI QGIKIKAPRD SPNTDGIDIF ASKRFEIEKC
260 270 280 290 300
TIGTGDDCVA VGTGSSNITI KDLTCGPGHG MSIGSLGKGN SRSEVSFVHL
310 320 330 340 350
DGAKFIDTQN GLRIKTWQGG SGLASHITYE NVEMINAENP ILINQFYCTS
360 370 380 390 400
AAACKNQRSA VKIQDVTFKN IHGTSATTAA IQLMCSDSVP CSNIKLSNVF
410 420 430 440 450
LKLTSGKVAT CVNKNANGYY TNPLNPSCKS LHPGRTPKEL ELHQKPTTLL
460 470 480 490 500
MDEKMGASLN SSPPNCKNKC KGCQPCKPKL IIVHPNQPED YYPQRWVCSC

HNKIYNP
Length:507
Mass (Da):55,731
Last modified:March 1, 2001 - v1
Checksum:i2B2E0AA5E958FE5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ404653 mRNA. Translation: CAC05582.1.
PIRiJC7366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ404653 mRNA. Translation: CAC05582.1.
PIRiJC7366.

3D structure databases

ProteinModelPortaliQ9FY19.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3339. Jun a 2.0101.
428. Jun a 2.
CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR2_JUNAS
AccessioniPrimary (citable) accession number: Q9FY19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.