Reviewed, UniProtKB/Swiss-Prot Q9FY06 (PPF1_PEA)

Names and origin

Protein names

Recommended name:
    Inner membrane protein PPF-1, chloroplastic
Alternative name(s):
    Post-floral-specific protein 1

Gene names

Name:

PPF-1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be required for the insertion of some integral membrane proteins into the chloroplast thylakoid membrane. May play a role in inhibiting senescence.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein Potential.
Tissue specificity	Highly expressed in apical buds. Low levels of expression in leaves. Not expressed in roots, and stems.
Developmental stage	Expressed after flower initiation.
Induction	By the plant hormone gibberellin A3. Expression is induced to a greater extent by short day conditions than long day conditions.
Sequence similarities	Belongs to the OXA1/oxaA family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid
Coding sequence diversity	Polymorphism
Domain	Transit peptide Transmembrane
Gene Ontology (GO)
Biological process	protein insertion into membrane Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 442		Inner membrane protein PPF-1, chloroplastic		PRO_0000020370
Regions
	Topological domain	1 – 108	108	Lumenal Potential
	Transmembrane	109 – 129	21	Potential
	Topological domain	130 – 183	54	Stromal Potential
	Transmembrane	184 – 204	21	Potential
	Topological domain	205 – 296	92	Lumenal Potential
	Transmembrane	297 – 317	21	Potential
	Topological domain	318 – 442	125	Stromal Potential
Natural variations
	Natural variant	107	1	D → G in strain: cv. Alaska. Ref.2
	Natural variant	127	1	V → G in strain: cv. Alaska. Ref.2
	Natural variant	150	1	L → P in strain: cv. Alaska. Ref.2
	Natural variant	250	1	L → P in strain: cv. Alaska. Ref.2
	Natural variant	326 – 327	2	QQ → HK in strain: cv. Alaska.

Sequences

	Sequence		Length	Mass (Da)
	Q9FY06-1 [UniParc]. Last modified November 14, 2003. Version 2. Checksum: 3D4D4C99C5BE9B04	FASTA	442	48,238
	10 20 30 40 50 60 MAKTLISSPS FLGTPLPSLH RTFSPNRTRL FTKVQFSFHQ LPPIQSVSHS VDLSGIFARA 70 80 90 100 110 120 EGLLYTLADA TVAADAAAST DVAAQKNGGW FGFISDGMEF VLKVLKDGLS SVHVPYSYGF 130 140 150 160 170 180 AIILLTVIVK AATLPLTKQQ VESTLAMQNL QPKIKAIQER YAGNQERIQL ETSRLYTQAG 190 200 210 220 230 240 VNPLAGCLPT LATIPVWIGL YQALSNVANE GLLTEGFLWI PSLGGPTSIA ARQSGSGISW 250 260 270 280 290 300 LFPFVDGHPL LGWYDTAAYL VLPVLLIVSQ YVSMEIMKPP QTNDPNQKNT LLIFKFLPLM 310 320 330 340 350 360 IGYFSLSVPS GLTIYWFTNN VLSTAQQVWL RKLGGAKPAV NENAGGIITA GQAKRSASKP 370 380 390 400 410 420 EKGGERFRQL KEEEKKKKLI KALPVEEVQP LASASASNDG SDVENNKEQE VTEESNTSKV 430 440 SQEVQSFSRE RRSKRSKRKP VA

References

[1]	"PPF-1, a post-floral-specific gene expressed in short-day-grown G2 pea, may be important for its never-senescing phenotype." Zhu Y., Zhang Y., Luo J., Davies P.J., Ho D.T.-H. Gene 208:1-6(1998) [PubMed: 9479033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. G2. Tissue: Apical bud.
[2]	"Cloning and sequencing of the homologous gene of PPF-1 from Alaska pea." Xu Y., Wang M., Li Q., Ji X., Zhu Y. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-107; GLY-127; PRO-150; PRO-250 AND 326-HIS-LYS-327. Strain: cv. Alaska.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y12618 mRNA. Translation: CAA73179.1. AJ297606 mRNA. Translation: CAC04249.1.
PIR	T06476.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR020001. Membrane_insert_OxaA/YidC_core. IPR001708. Membrane_insertion_OxaA/YidC. [Graphical view]
PANTHER	PTHR12428. Innermemb_insert. 1 hit.
Pfam	PF02096. 60KD_IMP. 1 hit. [Graphical view]
TIGRFAMs	TIGR03592. YidC_oxa1_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPF1_PEA

Accession

Primary (citable) accession number: Q9FY06
Secondary accession number(s): O04699

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	November 14, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)