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Q9FXT4

- AGAL_ORYSJ

UniProt

Q9FXT4 - AGAL_ORYSJ

Protein

Alpha-galactosidase

Gene

Os10g0493600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Hydrolyzes melibiose, raffinose and stachyose in the following decreasing order of reactivity: raffinose, melibiose, stachyose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

    Enzyme regulationi

    1 mM Hg2+ and Ag2+ decrease activity by 98% and 96% respectively. 1 mM Para-chloromercuribenzoic acid (PCMB) completely inhibits enzymatic activity.1 Publication

    pH dependencei

    Optimum pH is 5.0. Slowly inactivated above pH 8.0 and below pH 3.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius with p-nitrophenyl-alpha-D-galactopyranoside as substrate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei185 – 1851NucleophileBy similarity
    Active sitei240 – 2401Proton donorBy similarity

    GO - Molecular functioni

    1. raffinose alpha-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. leaf morphogenesis Source: EnsemblPlants/Gramene
    3. positive regulation of flower development Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-galactosidase (EC:3.2.1.22)
    Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
    Gene namesi
    Ordered Locus Names:Os10g0493600, LOC_Os10g35110
    ORF Names:OSJNBa0041P03
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 10

    Organism-specific databases

    GrameneiQ9FXT4.

    Subcellular locationi

    GO - Cellular componenti

    1. plant-type cell wall Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 55551 PublicationAdd
    BLAST
    Chaini56 – 417362Alpha-galactosidasePRO_0000001000Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi76 ↔ 108By similarity
    Disulfide bondi156 ↔ 187By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    IntActiQ9FXT4. 9 interactions.
    STRINGi39947.LOC_Os10g35110.2.

    Structurei

    Secondary structure

    1
    417
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi66 – 705
    Helixi71 – 744
    Helixi80 – 9213
    Helixi95 – 984
    Beta strandi102 – 1043
    Turni124 – 1263
    Helixi131 – 14010
    Beta strandi144 – 15411
    Beta strandi158 – 1614
    Helixi167 – 17711
    Beta strandi181 – 1855
    Helixi194 – 20815
    Beta strandi212 – 2187
    Turni219 – 2224
    Helixi224 – 2263
    Helixi228 – 2303
    Beta strandi233 – 2364
    Helixi245 – 25612
    Helixi257 – 2615
    Beta strandi266 – 2694
    Beta strandi277 – 2804
    Helixi282 – 29413
    Beta strandi299 – 3013
    Helixi310 – 3167
    Helixi319 – 3257
    Beta strandi334 – 3396
    Beta strandi342 – 3487
    Beta strandi354 – 3607
    Beta strandi362 – 3643
    Beta strandi366 – 3716
    Helixi372 – 3743
    Beta strandi382 – 3876
    Turni388 – 3914
    Beta strandi392 – 40413
    Beta strandi409 – 4168

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UASX-ray1.50A56-417[»]
    ProteinModelPortaliQ9FXT4.
    SMRiQ9FXT4. Positions 56-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9FXT4.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2225Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    KOiK07407.
    OMAiRSVMERY.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FXT4-1 [UniParc]FASTAAdd to Basket

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    MARASSSSSP PSPRLLLLLL VAVAATLLPE AAALGNFTAE SRGARWRSRR    50
    ARRRAFENGL GRTPQMGWNS WNHFYCGINE QIIRETADAL VNTGLAKLGY 100
    QYVNIDDCWA EYSRDSQGNF VPNRQTFPSG IKALADYVHA KGLKLGIYSD 150
    AGSQTCSNKM PGSLDHEEQD VKTFASWGVD YLKYDNCNDA GRSVMERYTR 200
    MSNAMKTYGK NIFFSLCEWG KENPATWAGR MGNSWRTTGD IADNWGSMTS 250
    RADENDQWAA YAGPGGWNDP DMLEVGNGGM SEAEYRSHFS IWALAKAPLL 300
    IGCDVRSMSQ QTKNILSNSE VIAVNQDSLG VQGKKVQSDN GLEVWAGPLS 350
    NNRKAVVLWN RQSYQATITA HWSNIGLAGS VAVTARDLWA HSSFAAQGQI 400
    SASVAPHDCK MYVLTPN 417
    Length:417
    Mass (Da):45,821
    Last modified:March 1, 2001 - v1
    Checksum:i5F7B16326C042BF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039671 mRNA. Translation: BAB12570.1.
    AC068950 Genomic DNA. Translation: AAM92832.1.
    DP000086 Genomic DNA. Translation: AAP54412.1.
    AP008216 Genomic DNA. Translation: BAF26853.2.
    AF251064 mRNA. Translation: AAO85428.1.
    RefSeqiNP_001064939.2. NM_001071474.2.
    UniGeneiOs.88093.

    Genome annotation databases

    EnsemblPlantsiOS10T0493600-01; OS10T0493600-01; OS10G0493600.
    GeneIDi4348988.
    KEGGiosa:4348988.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039671 mRNA. Translation: BAB12570.1 .
    AC068950 Genomic DNA. Translation: AAM92832.1 .
    DP000086 Genomic DNA. Translation: AAP54412.1 .
    AP008216 Genomic DNA. Translation: BAF26853.2 .
    AF251064 mRNA. Translation: AAO85428.1 .
    RefSeqi NP_001064939.2. NM_001071474.2.
    UniGenei Os.88093.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UAS X-ray 1.50 A 56-417 [» ]
    ProteinModelPortali Q9FXT4.
    SMRi Q9FXT4. Positions 56-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9FXT4. 9 interactions.
    STRINGi 39947.LOC_Os10g35110.2.

    Chemistry

    ChEMBLi CHEMBL4537.

    Protein family/group databases

    CAZyi GH27. Glycoside Hydrolase Family 27.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS10T0493600-01 ; OS10T0493600-01 ; OS10G0493600 .
    GeneIDi 4348988.
    KEGGi osa:4348988.

    Organism-specific databases

    Gramenei Q9FXT4.

    Phylogenomic databases

    eggNOGi NOG68897.
    KOi K07407.
    OMAi RSVMERY.

    Miscellaneous databases

    EvolutionaryTracei Q9FXT4.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alpha-galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells."
      Kim W.-D., Kobayashi O., Kaneko S., Sakakibara Y., Park G.-G., Kusakabe I., Tanaka H., Kobayashi H.
      Phytochemistry 61:621-630(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 56-95, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: cv. Nipponbare.
      Tissue: Callus.
    2. "In-depth view of structure, activity, and evolution of rice chromosome 10."
      Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H.
      , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.
      Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. Lee R.H., Chen S.C.G.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 101-284.
      Strain: cv. Tainung 67.
      Tissue: Leaf.
    6. "Crystal structure of rice alpha-galactosidase complexed with D-galactose."
      Fujimoto Z., Kaneko S., Momma M., Kobayashi H., Mizuno H.
      J. Biol. Chem. 278:20313-20318(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 56-417 IN COMPLEX WITH D-GALACTOSE.

    Entry informationi

    Entry nameiAGAL_ORYSJ
    AccessioniPrimary (citable) accession number: Q9FXT4
    Secondary accession number(s): Q0IWR2, Q84UX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3