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Protein

Alpha-galactosidase

Gene

Os10g0493600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes melibiose, raffinose and stachyose in the following decreasing order of reactivity: raffinose, melibiose, stachyose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Enzyme regulationi

1 mM Hg2+ and Ag2+ decrease activity by 98% and 96% respectively. 1 mM Para-chloromercuribenzoic acid (PCMB) completely inhibits enzymatic activity.1 Publication

pH dependencei

Optimum pH is 5.0. Slowly inactivated above pH 8.0 and below pH 3.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius with p-nitrophenyl-alpha-D-galactopyranoside as substrate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185NucleophileBy similarity1
Active sitei240Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.22. 4460.
ReactomeiR-OSA-1660662. Glycosphingolipid metabolism.
R-OSA-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
Gene namesi
Ordered Locus Names:Os10g0493600, LOC_Os10g35110
ORF Names:OSJNBa0041P03
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4537.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 551 PublicationAdd BLAST55
ChainiPRO_000000100056 – 417Alpha-galactosidaseAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi76 ↔ 108By similarity
Disulfide bondi156 ↔ 187By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FXT4.
PRIDEiQ9FXT4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FXT4. baseline and differential.
GenevisibleiQ9FXT4. OS.

Interactioni

Protein-protein interaction databases

IntActiQ9FXT4. 9 interactors.
STRINGi39947.LOC_Os10g35110.1.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi66 – 70Combined sources5
Helixi71 – 74Combined sources4
Helixi80 – 92Combined sources13
Helixi95 – 98Combined sources4
Beta strandi102 – 104Combined sources3
Turni124 – 126Combined sources3
Helixi131 – 140Combined sources10
Beta strandi144 – 154Combined sources11
Beta strandi158 – 161Combined sources4
Helixi167 – 177Combined sources11
Beta strandi181 – 185Combined sources5
Helixi194 – 208Combined sources15
Beta strandi212 – 218Combined sources7
Turni219 – 222Combined sources4
Helixi224 – 226Combined sources3
Helixi228 – 230Combined sources3
Beta strandi233 – 236Combined sources4
Helixi245 – 256Combined sources12
Helixi257 – 261Combined sources5
Beta strandi266 – 269Combined sources4
Beta strandi277 – 280Combined sources4
Helixi282 – 294Combined sources13
Beta strandi299 – 301Combined sources3
Helixi310 – 316Combined sources7
Helixi319 – 325Combined sources7
Beta strandi334 – 339Combined sources6
Beta strandi342 – 348Combined sources7
Beta strandi354 – 360Combined sources7
Beta strandi362 – 364Combined sources3
Beta strandi366 – 371Combined sources6
Helixi372 – 374Combined sources3
Beta strandi382 – 387Combined sources6
Turni388 – 391Combined sources4
Beta strandi392 – 404Combined sources13
Beta strandi409 – 416Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UASX-ray1.50A56-417[»]
ProteinModelPortaliQ9FXT4.
SMRiQ9FXT4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9FXT4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 222Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
InParanoidiQ9FXT4.
KOiK07407.
OMAiKENPATW.
OrthoDBiEOG09360A7S.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FXT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARASSSSSP PSPRLLLLLL VAVAATLLPE AAALGNFTAE SRGARWRSRR
60 70 80 90 100
ARRRAFENGL GRTPQMGWNS WNHFYCGINE QIIRETADAL VNTGLAKLGY
110 120 130 140 150
QYVNIDDCWA EYSRDSQGNF VPNRQTFPSG IKALADYVHA KGLKLGIYSD
160 170 180 190 200
AGSQTCSNKM PGSLDHEEQD VKTFASWGVD YLKYDNCNDA GRSVMERYTR
210 220 230 240 250
MSNAMKTYGK NIFFSLCEWG KENPATWAGR MGNSWRTTGD IADNWGSMTS
260 270 280 290 300
RADENDQWAA YAGPGGWNDP DMLEVGNGGM SEAEYRSHFS IWALAKAPLL
310 320 330 340 350
IGCDVRSMSQ QTKNILSNSE VIAVNQDSLG VQGKKVQSDN GLEVWAGPLS
360 370 380 390 400
NNRKAVVLWN RQSYQATITA HWSNIGLAGS VAVTARDLWA HSSFAAQGQI
410
SASVAPHDCK MYVLTPN
Length:417
Mass (Da):45,821
Last modified:March 1, 2001 - v1
Checksum:i5F7B16326C042BF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039671 mRNA. Translation: BAB12570.1.
AC068950 Genomic DNA. Translation: AAM92832.1.
DP000086 Genomic DNA. Translation: AAP54412.1.
DP000086 Genomic DNA. Translation: ABB47819.1.
AP008216 Genomic DNA. Translation: BAF26853.2.
AP014966 Genomic DNA. Translation: BAT11449.1.
AF251064 mRNA. Translation: AAO85428.1.
RefSeqiXP_015613565.1. XM_015758079.1.
XP_015613566.1. XM_015758080.1.
UniGeneiOs.88093.

Genome annotation databases

EnsemblPlantsiOS10T0493600-01; OS10T0493600-01; OS10G0493600.
GeneIDi4348988.
GrameneiOS10T0493600-01; OS10T0493600-01; OS10G0493600.
KEGGiosa:4348988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039671 mRNA. Translation: BAB12570.1.
AC068950 Genomic DNA. Translation: AAM92832.1.
DP000086 Genomic DNA. Translation: AAP54412.1.
DP000086 Genomic DNA. Translation: ABB47819.1.
AP008216 Genomic DNA. Translation: BAF26853.2.
AP014966 Genomic DNA. Translation: BAT11449.1.
AF251064 mRNA. Translation: AAO85428.1.
RefSeqiXP_015613565.1. XM_015758079.1.
XP_015613566.1. XM_015758080.1.
UniGeneiOs.88093.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UASX-ray1.50A56-417[»]
ProteinModelPortaliQ9FXT4.
SMRiQ9FXT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9FXT4. 9 interactors.
STRINGi39947.LOC_Os10g35110.1.

Chemistry databases

ChEMBLiCHEMBL4537.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Proteomic databases

PaxDbiQ9FXT4.
PRIDEiQ9FXT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS10T0493600-01; OS10T0493600-01; OS10G0493600.
GeneIDi4348988.
GrameneiOS10T0493600-01; OS10T0493600-01; OS10G0493600.
KEGGiosa:4348988.

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
InParanoidiQ9FXT4.
KOiK07407.
OMAiKENPATW.
OrthoDBiEOG09360A7S.

Enzyme and pathway databases

BRENDAi3.2.1.22. 4460.
ReactomeiR-OSA-1660662. Glycosphingolipid metabolism.
R-OSA-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ9FXT4.

Gene expression databases

ExpressionAtlasiQ9FXT4. baseline and differential.
GenevisibleiQ9FXT4. OS.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL_ORYSJ
AccessioniPrimary (citable) accession number: Q9FXT4
Secondary accession number(s): F4MGX5, Q0IWR2, Q84UX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.