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Protein

Alpha-galactosidase

Gene

Os10g0493600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes melibiose, raffinose and stachyose in the following decreasing order of reactivity: raffinose, melibiose, stachyose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Enzyme regulationi

1 mM Hg2+ and Ag2+ decrease activity by 98% and 96% respectively. 1 mM Para-chloromercuribenzoic acid (PCMB) completely inhibits enzymatic activity.1 Publication

pH dependencei

Optimum pH is 5.0. Slowly inactivated above pH 8.0 and below pH 3.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius with p-nitrophenyl-alpha-D-galactopyranoside as substrate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185NucleophileBy similarity1
Active sitei240Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.22 4460
ReactomeiR-OSA-1660662 Glycosphingolipid metabolism
R-OSA-6798695 Neutrophil degranulation

Protein family/group databases

CAZyiGH27 Glycoside Hydrolase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
Gene namesi
Ordered Locus Names:Os10g0493600, LOC_Os10g35110
ORF Names:OSJNBa0041P03
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4537

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 551 PublicationAdd BLAST55
ChainiPRO_000000100056 – 417Alpha-galactosidaseAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi76 ↔ 108By similarity
Disulfide bondi156 ↔ 187By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FXT4
PRIDEiQ9FXT4

Expressioni

Gene expression databases

ExpressionAtlasiQ9FXT4 baseline and differential
GenevisibleiQ9FXT4 OS

Interactioni

Protein-protein interaction databases

IntActiQ9FXT4 9 interactors.
STRINGi39947.LOC_Os10g35110.1

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi66 – 70Combined sources5
Helixi71 – 74Combined sources4
Helixi80 – 92Combined sources13
Helixi95 – 98Combined sources4
Beta strandi102 – 104Combined sources3
Turni124 – 126Combined sources3
Helixi131 – 140Combined sources10
Beta strandi144 – 154Combined sources11
Beta strandi158 – 161Combined sources4
Helixi167 – 177Combined sources11
Beta strandi181 – 185Combined sources5
Helixi194 – 208Combined sources15
Beta strandi212 – 218Combined sources7
Turni219 – 222Combined sources4
Helixi224 – 226Combined sources3
Helixi228 – 230Combined sources3
Beta strandi233 – 236Combined sources4
Helixi245 – 256Combined sources12
Helixi257 – 261Combined sources5
Beta strandi266 – 269Combined sources4
Beta strandi277 – 280Combined sources4
Helixi282 – 294Combined sources13
Beta strandi299 – 301Combined sources3
Helixi310 – 316Combined sources7
Helixi319 – 325Combined sources7
Beta strandi334 – 339Combined sources6
Beta strandi342 – 348Combined sources7
Beta strandi354 – 360Combined sources7
Beta strandi362 – 364Combined sources3
Beta strandi366 – 371Combined sources6
Helixi372 – 374Combined sources3
Beta strandi382 – 387Combined sources6
Turni388 – 391Combined sources4
Beta strandi392 – 404Combined sources13
Beta strandi409 – 416Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UASX-ray1.50A56-417[»]
ProteinModelPortaliQ9FXT4
SMRiQ9FXT4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9FXT4

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 222Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366 Eukaryota
ENOG410XPF1 LUCA
InParanoidiQ9FXT4
KOiK07407
OMAiSQFITRV
OrthoDBiEOG09360A7S

Family and domain databases

CDDicd14792 GH27, 1 hit
Gene3Di2.60.40.11801 hit
3.20.20.701 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002241 Glyco_hydro_27
IPR000111 Glyco_hydro_27/36_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR035373 Melibiase/NAGA_C
PfamiView protein in Pfam
PF16499 Melibiase_2, 1 hit
PF17450 Melibiase_2_C, 1 hit
PRINTSiPR00740 GLHYDRLASE27
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FXT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARASSSSSP PSPRLLLLLL VAVAATLLPE AAALGNFTAE SRGARWRSRR
60 70 80 90 100
ARRRAFENGL GRTPQMGWNS WNHFYCGINE QIIRETADAL VNTGLAKLGY
110 120 130 140 150
QYVNIDDCWA EYSRDSQGNF VPNRQTFPSG IKALADYVHA KGLKLGIYSD
160 170 180 190 200
AGSQTCSNKM PGSLDHEEQD VKTFASWGVD YLKYDNCNDA GRSVMERYTR
210 220 230 240 250
MSNAMKTYGK NIFFSLCEWG KENPATWAGR MGNSWRTTGD IADNWGSMTS
260 270 280 290 300
RADENDQWAA YAGPGGWNDP DMLEVGNGGM SEAEYRSHFS IWALAKAPLL
310 320 330 340 350
IGCDVRSMSQ QTKNILSNSE VIAVNQDSLG VQGKKVQSDN GLEVWAGPLS
360 370 380 390 400
NNRKAVVLWN RQSYQATITA HWSNIGLAGS VAVTARDLWA HSSFAAQGQI
410
SASVAPHDCK MYVLTPN
Length:417
Mass (Da):45,821
Last modified:March 1, 2001 - v1
Checksum:i5F7B16326C042BF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039671 mRNA Translation: BAB12570.1
AC068950 Genomic DNA Translation: AAM92832.1
DP000086 Genomic DNA Translation: AAP54412.1
DP000086 Genomic DNA Translation: ABB47819.1
AP008216 Genomic DNA Translation: BAF26853.2
AP014966 Genomic DNA Translation: BAT11449.1
AF251064 mRNA Translation: AAO85428.1
RefSeqiXP_015613565.1, XM_015758079.1
XP_015613566.1, XM_015758080.1
UniGeneiOs.88093

Genome annotation databases

EnsemblPlantsiOs10t0493600-01; Os10t0493600-01; Os10g0493600
Os10t0493600-03; Os10t0493600-03; Os10g0493600
GeneIDi4348988
GrameneiOs10t0493600-01; Os10t0493600-01; Os10g0493600
Os10t0493600-03; Os10t0493600-03; Os10g0493600
KEGGiosa:4348988

Similar proteinsi

Entry informationi

Entry nameiAGAL_ORYSJ
AccessioniPrimary (citable) accession number: Q9FXT4
Secondary accession number(s): F4MGX5, Q0IWR2, Q84UX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2001
Last modified: February 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome