Reviewed, UniProtKB/Swiss-Prot Q9FXI9 (GUN2_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 2
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 2

Gene names

Ordered Locus Names:	At1g19940
ORF Names:	F6F9.1, T20H2.27

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 515	484	Endoglucanase 2		PRO_0000249255
Sites
	Active site	433	1	By similarity
	Active site	480	1	By similarity
	Active site	489	1	By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FXI9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F21BEC7479B5E209	FASTA	515	56,708
	10 20 30 40 50 60 MVAKPRSRCC CCSVFIGVII LIAIIIAVIF TIRHRSNHSD DDGSNVKNYA NALKIAMQFF 70 80 90 100 110 120 DIQKSGKLEN NEISWRGDSG LKDGSEASID LSKGLYDAGD HMKFGFPMAF TATVLSWSIL 130 140 150 160 170 180 EYGDQMASLN LLDHAKDSLK WTTDFLINAH PSPNVLYIQV GDPVTDHKCW DRPETMTRKR 190 200 210 220 230 240 TLTKIDTKTP GTEVAAETAA AMAAASLVFK ESDTKYSSTL LKHAKQLFDF ADNNRGSYSV 250 260 270 280 290 300 NIPEVQSYYN STGYGDELLW AASWLYHATE DQTYLDFVSE NGEEFGNFGS PSWFSWDNKL 310 320 330 340 350 360 PGTHILLSRL TFFKKGLSGS KGLQGFKETA EAVMCGLIPS SPTATSSRTD GGLIWVSEWN 370 380 390 400 410 420 ALQHPVSSAF LATLYSDYML TSGVKELSCS DQSFKPSDLR KFARSQADYM LGKNPEKMSY 430 440 450 460 470 480 LVGYGEKYPE FVHHRGASIP ADATTGCKDG FKWLNSDEPN PNVAYGALVG GPFLNDTFID 490 500 510 ARNNSMQNEP STYNSALVVG LLSSLVTTSS SVESF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC022472 Genomic DNA. Translation: AAF79918.1. AC007797 Genomic DNA. Translation: AAG12562.1. AY048245 mRNA. Translation: AAK82507.1. AY113063 mRNA. Translation: AAM47371.1.
IPI	IPI00541042.
PIR	G86332.
RefSeq	NP_173423.1.
UniGene	At.16213
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q9FXI9.
Genome annotation databases
GeneID	838583.
GenomeReviews	Gene locus AT1G19940 in contig CT485782_GR.
KEGG	ath:AT1G19940.
NMPDR	fig|3702.1.peg.2342.
Organism-specific databases
TAIR	At1g19940.
Phylogenomic databases
OMA	WASARYS.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
Genevestigator	Q9FXI9.
GermOnline	AT1G19940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN2_ARATH

Accession

Primary (citable) accession number: Q9FXI9
Secondary accession number(s): Q9LNS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)