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Q9FXE5 (FUCO3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-L-fucosidase 3

EC=3.2.1.51
Alternative name(s):
Alpha-1,2-fucosidase
Short name=AtFXG1
Alpha-L-fucoside fucohydrolase 3
Gene names
Name:FXG1
Ordered Locus Names:At1g67830
ORF Names:F12A21.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. Ref.4

Catalytic activity

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Subcellular location

Secretedextracellular spaceapoplast Ref.4 Ref.5.

Tissue specificity

High expression in younger leaves and in the apical region of the inflorescence stem. Ref.5

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family.

Caution

The functional assignment is controversial.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

plant-type cell wall

Inferred from direct assay PubMed 17526915. Source: TAIR

   Molecular_functionalpha-L-fucosidase activity

Inferred from direct assay Ref.4. Source: TAIR

hydrolase activity, acting on ester bonds

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 372349Alpha-L-fucosidase 3
PRO_0000225694

Sites

Active site371Nucleophile By similarity
Active site3451 By similarity
Active site3481 By similarity

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict111L → R in BAD43265. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9FXE5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D88CA3FEE74A8155

FASTA37240,462
        10         20         30         40         50         60 
MNPILSSLFA LSLLSSLSPS THAHQCHFPA IFNFGDSNSD TGGLSAAFGQ AGPPHGSSFF 

        70         80         90        100        110        120 
GSPAGRYCDG RLVIDFIAES LGLPYLSAFL DSVGSNFSHG ANFATAGSPI RALNSTLRQS 

       130        140        150        160        170        180 
GFSPFSLDVQ FVQFYNFHNR SQTVRSRGGV YKTMLPESDS FSKALYTFDI GQNDLTAGYF 

       190        200        210        220        230        240 
ANKTVEQVET EVPEIISQFM NAIKNIYGQG GRYFWIHNTG PIGCLAYVIE RFPNKASDFD 

       250        260        270        280        290        300 
SHGCVSPLNH LAQQFNHALK QAVIELRSSL SEAAITYVDV YSLKHELFVH AQGHGFKGSL 

       310        320        330        340        350        360 
VSCCGHGGKY NYNKGIGCGM KKIVKGKEVY IGKPCDEPDK AVVWDGVHFT QAANKFIFDK 

       370 
IAPGLSKACK RQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-372.
Strain: cv. Columbia.
[4]"AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides."
de La Torre F., Sampedro J., Zarra I., Revilla G.
Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC008113 Genomic DNA. Translation: AAG28886.1.
CP002684 Genomic DNA. Translation: AEE34703.1.
AK175502 mRNA. Translation: BAD43265.1.
RefSeqNP_176949.1. NM_105451.3.
UniGeneAt.23529.
At.35596.

3D structure databases

ProteinModelPortalQ9FXE5.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9FXE5.
PRIDEQ9FXE5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G67830.1; AT1G67830.1; AT1G67830.
GeneID843109.
KEGGath:AT1G67830.

Organism-specific databases

TAIRAT1G67830.

Phylogenomic databases

eggNOGNOG245146.
HOGENOMHOG000237648.
InParanoidQ9FXE5.
KOK01206.
OMAKRRRCQA.
PhylomeDBQ9FXE5.

Enzyme and pathway databases

BioCycARA:AT1G67830-MONOMER.
MetaCyc:AT1G67830-MONOMER.

Gene expression databases

GenevestigatorQ9FXE5.

Family and domain databases

Gene3D3.40.50.1110. 1 hit.
InterProIPR001087. Lipase_GDSL.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamPF00657. Lipase_GDSL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUCO3_ARATH
AccessionPrimary (citable) accession number: Q9FXE5
Secondary accession number(s): Q681W5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names