Q9FXE5 (FUCO3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-L-fucosidase 3
EC=3.2.1.51

Alternative name(s):
Alpha-1,2-fucosidase
Short name=AtFXG1
Alpha-L-fucoside fucohydrolase 3

Gene names

Name:	FXG1
Ordered Locus Names:	At1g67830
ORF Names:	F12A21.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. Ref.4
Catalytic activity	An alpha-L-fucoside + H2O = L-fucose + an alcohol.
Subcellular location	Secreted › extracellular space › apoplast Ref.4 Ref.5.
Tissue specificity	High expression in younger leaves and in the apical region of the inflorescence stem. Ref.5
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family.
Caution	The functional assignment is controversial.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay PubMed 17526915. Source: TAIR
Molecular_function	alpha-L-fucosidase activity Inferred from direct assay Ref.4. Source: TAIR hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 372	349	Alpha-L-fucosidase 3		PRO_0000225694
Sites
	Active site	37	1	Nucleophile By similarity
	Active site	345	1	By similarity
	Active site	348	1	By similarity
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	11	1	L → R in BAD43265. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9FXE5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D88CA3FEE74A8155	FASTA	372	40,462
	10 20 30 40 50 60 MNPILSSLFA LSLLSSLSPS THAHQCHFPA IFNFGDSNSD TGGLSAAFGQ AGPPHGSSFF 70 80 90 100 110 120 GSPAGRYCDG RLVIDFIAES LGLPYLSAFL DSVGSNFSHG ANFATAGSPI RALNSTLRQS 130 140 150 160 170 180 GFSPFSLDVQ FVQFYNFHNR SQTVRSRGGV YKTMLPESDS FSKALYTFDI GQNDLTAGYF 190 200 210 220 230 240 ANKTVEQVET EVPEIISQFM NAIKNIYGQG GRYFWIHNTG PIGCLAYVIE RFPNKASDFD 250 260 270 280 290 300 SHGCVSPLNH LAQQFNHALK QAVIELRSSL SEAAITYVDV YSLKHELFVH AQGHGFKGSL 310 320 330 340 350 360 VSCCGHGGKY NYNKGIGCGM KKIVKGKEVY IGKPCDEPDK AVVWDGVHFT QAANKFIFDK 370 IAPGLSKACK RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-372. Strain: cv. Columbia.
[4]	"AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides." de La Torre F., Sampedro J., Zarra I., Revilla G. Plant Physiol. 128:247-255(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana." Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I. Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008113 Genomic DNA. Translation: AAG28886.1. CP002684 Genomic DNA. Translation: AEE34703.1. AK175502 mRNA. Translation: BAD43265.1.
IPI	IPI00521284.
RefSeq	NP_176949.1. NM_105451.3.
UniGene	At.23529. At.35596.
3D structure databases
ProteinModelPortal	Q9FXE5.
ModBase	Search...
Proteomic databases
PaxDb	Q9FXE5.
PRIDE	Q9FXE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G67830.1; AT1G67830.1; AT1G67830.
GeneID	843109.
KEGG	ath:AT1G67830.
Organism-specific databases
TAIR	At1g67830.
Phylogenomic databases
eggNOG	NOG245146.
HOGENOM	HOG000237648.
InParanoid	Q9FXE5.
KO	K01206.
OMA	GRYFWIH.
PhylomeDB	Q9FXE5.
ProtClustDB	CLSN2914480.
Enzyme and pathway databases
BioCyc	ARA:AT1G67830-MONOMER. MetaCyc:AT1G67830-MONOMER.
Gene expression databases
Genevestigator	Q9FXE5.
GermOnline	AT1G67830. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.50.1110. 1 hit.
InterPro	IPR001087. Lipase_GDSL. IPR013831. SGNH_hydro-type_esterase_dom. [Graphical view]
Pfam	PF00657. Lipase_GDSL. 1 hit. [Graphical view]
PROSITE	PS01098. LIPASE_GDSL_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUCO3_ARATH

Accession

Primary (citable) accession number: Q9FXE5
Secondary accession number(s): Q681W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program