Q9FXD8 (PLY5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase 5
EC=4.2.2.2

Gene names

Ordered Locus Names:	At1g67750
ORF Names:	F12A21.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.
Sequence caution	The sequence AAG28907.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 408	381	Probable pectate lyase 5		PRO_0000024869
Sites
	Active site	286	1	Potential
	Metal binding	206	1	Calcium By similarity
	Metal binding	230	1	Calcium By similarity
	Metal binding	234	1	Calcium By similarity
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	47	1	S → A in AAM67091. Ref.4
	Sequence conflict	94	1	R → L in AAM67091. Ref.4
	Sequence conflict	238	1	G → A in AAM67091. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9FXD8 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: B44DCF15C8B8E69B	FASTA	408	44,886
	10 20 30 40 50 60 MRMTLVHLSL SLFSCLLLVL SPTFIASTPV SEPELVVQEV NEKINASRRN LGVLSCGTGN 70 80 90 100 110 120 PIDDCWRCDP KWEKNRQRLA DCAIGFGKHA IGGRDGKIYV VTDSSDKDVV NPKPGTLRHA 130 140 150 160 170 180 VIQDEPLWII FARDMVIKLK EELIMNSFKT IDGRGASVHI AGGACITVQY VTNIIIHGVN 190 200 210 220 230 240 IHDCKRKGNA YVRDSPSHYG WRTASDGDAV SIFGGSHVWV DHCSLSNCAD GLIDAIHGST 250 260 270 280 290 300 AITISNNYLS HHNKVMLLGH SDSYTRDKNM QVTIAFNHFG EGLVQRMPRC RHGYFHVVNN 310 320 330 340 350 360 DYTHWQMYAI GGSAAPTINS QGNRFLAPND HVFKEVTKYE DAPRSKWKKW NWRSEGDLFL 370 380 390 400 NGAFFTPSGG GASSSYAKAS SLSARPSSLV ASVTSNAGAL FCRKGSRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-408.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008113 Genomic DNA. Translation: AAG28907.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE34690.1. AF462797 mRNA. Translation: AAL58893.1. AY101535 mRNA. Translation: AAM26656.1. AY088778 mRNA. Translation: AAM67091.1.
IPI	IPI00547564.
RefSeq	NP_564906.1. NM_105443.4.
UniGene	At.20989.
3D structure databases
ProteinModelPortal	Q9FXD8.
SMR	Q9FXD8. Positions 59-401.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q9FXD8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G67750.1; AT1G67750.1; AT1G67750.
GeneID	843100.
GenomeReviews	Gene locus AT1G67750 in contig CT485782_GR.
KEGG	ath:AT1G67750.
NMPDR	fig|3702.1.peg.6213.
Organism-specific databases
TAIR	At1g67750.
Phylogenomic databases
eggNOG	euNOG10883.
GeneTree	EPGT00050000008990.
HOGENOM	HBG317381.
InParanoid	Q9FXD8.
OMA	TRDKNMQ.
PhylomeDB	Q9FXD8.
ProtClustDB	CLSN2917394.
Gene expression databases
ArrayExpress	Q9FXD8.
Genevestigator	Q9FXD8.
GermOnline	AT1G67750. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY5_ARATH

Accession

Primary (citable) accession number: Q9FXD8
Secondary accession number(s): Q8L8V9, Q8W116

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	December 14, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program