Q9FX97 (FUT12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative fucosyltransferase-like protein

Alternative name(s):
FucT2
FucTB
Fucosyltransferase 12
Short name=AtFUT12

Gene names

Name:	FUT12
Ordered Locus Names:	At1g49710
ORF Names:	F14J22.8, F14J22_18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in cell wall biosynthesis. May act as a fucosyltransferase.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform Long (identifier: Q9FX97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9FX97-2)

The sequence of this isoform is not available.

Note: Unstable and lacks the key conserved region and thus would presumably lack the catalytic activity.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Putative fucosyltransferase-like protein		PRO_0000221125
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	61 – 513	453	Lumenal Potential
Amino acid modifications
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
	Glycosylation	493	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2122AEDACBB0E4E5	FASTA	513	57,308
	10 20 30 40 50 60 MGVFSNLRGP RAGATHDEFP ATNGSPSSSS SPSSSIKRKL SNLLPLCVAL VVIAEIGFLG 70 80 90 100 110 120 RLDKVALVDT LTDFFTQSPS LSQSPPARSD RKKIGLFTDR SCEEWLMRED SVTYSRDFTK 130 140 150 160 170 180 DPIFISGGEK DFQWCSVDCT FGDSSGKTPD AAFGLGQKPG TLSIIRSMES AQYYPENDLA 190 200 210 220 230 240 QARRRGYDIV MTTSLSSDVP VGYFSWAEYD IMSPVQPKTE RAIAAAFISN CGARNFRLQA 250 260 270 280 290 300 LEALMKTNIK IDSYGGCHRN RDGKVDKVEA LKRYKFSLAF ENTNEEDYVT EKFFQSLVAG 310 320 330 340 350 360 SVPVVVGPPN IEEFAPASDS FLHIKTMEDV EPVAKRMKYL AANPAAYNQT LRWKYEGPSD 370 380 390 400 410 420 SFKALVDMAA VHSSCRLCIF LATRVREQEE ESPNFKKRPC KCSRGGSDTV YHVFVRERGR 430 440 450 460 470 480 FEMESVFLRG KSVTQEALES AVLAKFKSLK HEAVWKKERP GNLKGDKELK IHRIYPLGLT 490 500 510 QRQALYNFKF EGNSSLSSHI QNNPCAKFEV VFV
	Isoform Short (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNAs encoding alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana." Wilson I.B., Rendic D., Freilinger A., Dumic J., Altmann F., Mucha J., Muller S., Hauser M.T. Biochim. Biophys. Acta 1527:88-96(2001) [PubMed: 11420147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Strain: cv. Columbia. Tissue: Root.
[2]	"Plant members of the alpha1-->3/4-fucosyltransferase gene family encode an alpha1-->4-fucosyltransferase, potentially involved in Lewis(a) biosynthesis, and two core alpha1-->3-fucosyltransferases." Bakker H., Schijlen E., de Vries T., Schiphorst W.E., Jordi W., Lommen A., Bosch D., van Die I. FEBS Lett. 507:307-312(2001) [PubMed: 11696361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Strain: cv. Columbia. Tissue: Silique.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ404861 mRNA. No translation available. AJ345085 mRNA. Translation: CAC78980.1. AC011807 Genomic DNA. Translation: AAG13053.1. CP002684 Genomic DNA. Translation: AEE32462.1. AY054522 mRNA. Translation: AAK96713.1. BT002570 mRNA. Translation: AAO00930.1.
IPI	IPI00548222.
PIR	F96533.
RefSeq	NP_175393.1. NM_103858.4.
UniGene	At.10376.
3D structure databases
ProteinModelPortal	Q9FX97.
ModBase	Search...
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Proteomic databases
PRIDE	Q9FX97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G49710.1; AT1G49710.1; AT1G49710.
GeneID	841394.
GenomeReviews	Gene locus AT1G49710 in contig CT485782_GR.
KEGG	ath:AT1G49710.
NMPDR	fig|3702.1.peg.4444.
Organism-specific databases
TAIR	At1g49710.
Phylogenomic databases
GeneTree	EPGT00070000030315.
HOGENOM	HBG317569.
InParanoid	Q9FX97.
OMA	NQSLRWK.
PhylomeDB	Q9FX97.
ProtClustDB	CLSN2682683.
Gene expression databases
ArrayExpress	Q9FX97.
Genevestigator	Q9FX97.
GermOnline	AT1G49710. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
KO	K00753.
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT12_ARATH

Accession

Primary (citable) accession number: Q9FX97

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program