Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial

Gene

At1g16700

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi129 – 1291Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi168 – 1681Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi172 – 1721Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT1G16700-MONOMER.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Ordered Locus Names:At1g16700
ORF Names:F19K19.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G16700.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 222NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrialPRO_0000410498
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PRIDEiQ9FX83.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

STRINGi3702.AT1G16700.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FX83.
SMRiQ9FX83. Positions 96-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 143304Fe-4S ferredoxin-type 1Add
BLAST
Domaini153 – 182304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000228289.
InParanoidiQ9FX83.
KOiK03941.
OMAiMAMSTRF.
PhylomeDBiQ9FX83.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FX83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLLARRSF SALRARHLAF SGQGLQGSHL CGLQSRAISY GSNKDDEEAE
60 70 80 90 100
QLAKEISKDW STVFERSMNT LFLTEMVRGL SLTLKYFFDP KVTINYPFEK
110 120 130 140 150
GPLSPRFRGE HALRRYPTGE ERCIACKLCE AVCPAQAITI EAEEREDGSR
160 170 180 190 200
RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL
210 220
LENGDRWETE IAENLRSESL YR
Length:222
Mass (Da):25,378
Last modified:March 1, 2001 - v1
Checksum:i64D8E9AFFF0BD5DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011808 Genomic DNA. Translation: AAG10813.1.
CP002684 Genomic DNA. Translation: AEE29484.1.
AY059922 mRNA. Translation: AAL24404.1.
AY081631 mRNA. Translation: AAM10193.1.
AY085448 mRNA. Translation: AAM62674.1.
PIRiC86302.
RefSeqiNP_173114.1. NM_101530.3.
UniGeneiAt.24368.

Genome annotation databases

EnsemblPlantsiAT1G16700.1; AT1G16700.1; AT1G16700.
GeneIDi838239.
KEGGiath:AT1G16700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011808 Genomic DNA. Translation: AAG10813.1.
CP002684 Genomic DNA. Translation: AEE29484.1.
AY059922 mRNA. Translation: AAL24404.1.
AY081631 mRNA. Translation: AAM10193.1.
AY085448 mRNA. Translation: AAM62674.1.
PIRiC86302.
RefSeqiNP_173114.1. NM_101530.3.
UniGeneiAt.24368.

3D structure databases

ProteinModelPortaliQ9FX83.
SMRiQ9FX83. Positions 96-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G16700.1.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEiQ9FX83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G16700.1; AT1G16700.1; AT1G16700.
GeneIDi838239.
KEGGiath:AT1G16700.

Organism-specific databases

GeneFarmi1825. 140.
TAIRiAT1G16700.

Phylogenomic databases

HOGENOMiHOG000228289.
InParanoidiQ9FX83.
KOiK03941.
OMAiMAMSTRF.
PhylomeDBiQ9FX83.

Enzyme and pathway databases

BioCyciARA:AT1G16700-MONOMER.

Miscellaneous databases

PROiQ9FX83.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiNDS8B_ARATH
AccessioniPrimary (citable) accession number: Q9FX83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2001
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.