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Q9FX83

- NDS8B_ARATH

UniProt

Q9FX83 - NDS8B_ARATH

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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial

Gene

At1g16700

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 2 4Fe-4S clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi123 – 1231Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi129 – 1291Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi168 – 1681Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi172 – 1721Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: TAIR
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT1G16700-MONOMER.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Ordered Locus Names:At1g16700
ORF Names:F19K19.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G16700.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: TAIR
  2. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 222NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrialPRO_0000410498
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PRIDEiQ9FX83.

Expressioni

Gene expression databases

GenevestigatoriQ9FX83.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

STRINGi3702.AT1G16700.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FX83.
SMRiQ9FX83. Positions 96-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 143304Fe-4S ferredoxin-type 1Add
BLAST
Domaini153 – 182304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000228289.
InParanoidiQ9FX83.
KOiK03941.
OMAiTYKYVNL.
PhylomeDBiQ9FX83.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FX83 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLLARRSF SALRARHLAF SGQGLQGSHL CGLQSRAISY GSNKDDEEAE
60 70 80 90 100
QLAKEISKDW STVFERSMNT LFLTEMVRGL SLTLKYFFDP KVTINYPFEK
110 120 130 140 150
GPLSPRFRGE HALRRYPTGE ERCIACKLCE AVCPAQAITI EAEEREDGSR
160 170 180 190 200
RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL
210 220
LENGDRWETE IAENLRSESL YR
Length:222
Mass (Da):25,378
Last modified:March 1, 2001 - v1
Checksum:i64D8E9AFFF0BD5DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011808 Genomic DNA. Translation: AAG10813.1.
CP002684 Genomic DNA. Translation: AEE29484.1.
AY059922 mRNA. Translation: AAL24404.1.
AY081631 mRNA. Translation: AAM10193.1.
AY085448 mRNA. Translation: AAM62674.1.
PIRiC86302.
RefSeqiNP_173114.1. NM_101530.3.
UniGeneiAt.24368.

Genome annotation databases

EnsemblPlantsiAT1G16700.1; AT1G16700.1; AT1G16700.
GeneIDi838239.
KEGGiath:AT1G16700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011808 Genomic DNA. Translation: AAG10813.1 .
CP002684 Genomic DNA. Translation: AEE29484.1 .
AY059922 mRNA. Translation: AAL24404.1 .
AY081631 mRNA. Translation: AAM10193.1 .
AY085448 mRNA. Translation: AAM62674.1 .
PIRi C86302.
RefSeqi NP_173114.1. NM_101530.3.
UniGenei At.24368.

3D structure databases

ProteinModelPortali Q9FX83.
SMRi Q9FX83. Positions 96-201.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G16700.1-P.

Protein family/group databases

TCDBi 3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEi Q9FX83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G16700.1 ; AT1G16700.1 ; AT1G16700 .
GeneIDi 838239.
KEGGi ath:AT1G16700.

Organism-specific databases

GeneFarmi 1825. 140.
TAIRi AT1G16700.

Phylogenomic databases

HOGENOMi HOG000228289.
InParanoidi Q9FX83.
KOi K03941.
OMAi TYKYVNL.
PhylomeDBi Q9FX83.

Enzyme and pathway databases

BioCyci ARA:AT1G16700-MONOMER.

Gene expression databases

Genevestigatori Q9FX83.

Family and domain databases

HAMAPi MF_01351. NDH1_NuoI.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view ]
Pfami PF12838. Fer4_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01971. NuoI. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiNDS8B_ARATH
AccessioniPrimary (citable) accession number: Q9FX83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3