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Protein

Trihelix transcription factor GT-1

Gene

GT-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor that binds specifically to the core DNA sequence 5'-GGTTAA-3'. May act as a molecular switch in response to light signals.3 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trihelix transcription factor GT-1
Alternative name(s):
Trihelix DNA-binding protein GT-1
Gene namesi
Name:GT-1
Ordered Locus Names:At1g13450
ORF Names:T6J4.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G13450.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86T → A: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi86T → D: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi89Q → P: Decreases strongly DNA-binding activity. 1 Publication1
Mutagenesisi118Q → P: Abolishes DNA-binding activity. 1 Publication1
Mutagenesisi133T → A: Decreases DNA-binding activity 2-fold. 2 Publications1
Mutagenesisi133T → D: Increases DNA-binding activity 3-fold. 2 Publications1
Mutagenesisi143L → P: Abolishes DNA-binding activity. 1 Publication1
Mutagenesisi175S → A: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi175S → D: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi179T → A: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi179T → D: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi198S → A: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi198S → D: Decreases DNA-binding activity 8-fold. 1 Publication1
Mutagenesisi278T → A: No significant change in DNA-binding activity. 1 Publication1
Mutagenesisi278T → D: No significant change in DNA-binding activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004013781 – 406Trihelix transcription factor GT-1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei133Phosphothreonine1 Publication1
Modified residuei198Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated on Thr-133 and Ser-198. Phosphorylation is calcium-dependent and increases DNA-binding activity 10 to 20-fold.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FX53.

PTM databases

iPTMnetiQ9FX53.

Expressioni

Gene expression databases

GenevisibleiQ9FX53. AT.

Interactioni

Subunit structurei

Binds DNA as homodimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9FX53. 1 interactor.
STRINGi3702.AT1G13450.1.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi85 – 87Combined sources3
Helixi89 – 108Combined sources20
Helixi113 – 127Combined sources15
Helixi132 – 146Combined sources15
Beta strandi152 – 154Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EBINMR-A81-166[»]
2JMWNMR-A81-166[»]
ProteinModelPortaliQ9FX53.
SMRiQ9FX53.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9FX53.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 143Myb-likePROSITE-ProRule annotationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 57His-richAdd BLAST12

Sequence similaritiesi

Contains 1 Myb-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IN2M. Eukaryota.
ENOG410XUBM. LUCA.
HOGENOMiHOG000064692.
InParanoidiQ9FX53.
OMAiFKKAKHH.
OrthoDBiEOG09360IMV.
PhylomeDBiQ9FX53.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR027775. C2H2_Znf_fam.
IPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
PROSITEiPS50090. MYB_LIKE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FX53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFISDKSRPT DFYKDDHHNS STTSTTRDMM IDVLTTTNES VDLQSHHHHN
60 70 80 90 100
HHNHHLHQSQ PQQQILLGES SGEDHEVKAP KKRAETWVQD ETRSLIMFRR
110 120 130 140 150
GMDGLFNTSK SNKHLWEQIS SKMREKGFDR SPTMCTDKWR NLLKEFKKAK
160 170 180 190 200
HHDRGNGSAK MSYYKEIEDI LRERSKKVTP PQYNKSPNTP PTSAKVDSFM
210 220 230 240 250
QFTDKGFDDT SISFGSVEAN GRPALNLERR LDHDGHPLAI TTAVDAVAAN
260 270 280 290 300
GVTPWNWRET PGNGDDSHGQ PFGGRVITVK FGDYTRRIGV DGSAEAIKEV
310 320 330 340 350
IRSAFGLRTR RAFWLEDEDQ IIRCLDRDMP LGNYLLRLDD GLAIRVCHYD
360 370 380 390 400
ESNQLPVHSE EKIFYTEEDY REFLARQGWS SLQVDGFRNI ENMDDLQPGA

VYRGVR
Length:406
Mass (Da):46,676
Last modified:March 1, 2001 - v1
Checksum:i0075E3A61B1CDE16
GO
Isoform 2 (identifier: Q9FX53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-263: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:361
Mass (Da):41,849
Checksum:i58F82DAB1E0B6990
GO
Isoform 3 (identifier: Q9FX53-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     265-278: DDSHGQPFGGRVIT → KSSEKAYINLSLKV
     279-406: Missing.

Note: No experimental confirmation available.
Show »
Length:278
Mass (Da):31,810
Checksum:iEDD30D9799AB575B
GO

Sequence cautioni

The sequence BX813511 differs from that shown. Sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti376R → L in AAA66473 (PubMed:7866025).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040173219 – 263Missing in isoform 2. CuratedAdd BLAST45
Alternative sequenceiVSP_040174265 – 278DDSHG…GRVIT → KSSEKAYINLSLKV in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_040175279 – 406Missing in isoform 3. 1 PublicationAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36806 mRNA. Translation: AAA66473.1.
AC011810 Genomic DNA. Translation: AAG09542.1.
CP002684 Genomic DNA. Translation: AEE29018.1.
CP002684 Genomic DNA. Translation: AEE29019.1.
CP002684 Genomic DNA. Translation: AEE29020.1.
AK228305 mRNA. Translation: BAF00248.1.
BX813511 mRNA. No translation available.
RefSeqiNP_001117279.1. NM_001123807.2. [Q9FX53-3]
NP_172802.2. NM_101215.4. [Q9FX53-1]
UniGeneiAt.430.
At.68029.

Genome annotation databases

EnsemblPlantsiAT1G13450.1; AT1G13450.1; AT1G13450. [Q9FX53-1]
GeneIDi837905.
GrameneiAT1G13450.1; AT1G13450.1; AT1G13450.
KEGGiath:AT1G13450.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36806 mRNA. Translation: AAA66473.1.
AC011810 Genomic DNA. Translation: AAG09542.1.
CP002684 Genomic DNA. Translation: AEE29018.1.
CP002684 Genomic DNA. Translation: AEE29019.1.
CP002684 Genomic DNA. Translation: AEE29020.1.
AK228305 mRNA. Translation: BAF00248.1.
BX813511 mRNA. No translation available.
RefSeqiNP_001117279.1. NM_001123807.2. [Q9FX53-3]
NP_172802.2. NM_101215.4. [Q9FX53-1]
UniGeneiAt.430.
At.68029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EBINMR-A81-166[»]
2JMWNMR-A81-166[»]
ProteinModelPortaliQ9FX53.
SMRiQ9FX53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9FX53. 1 interactor.
STRINGi3702.AT1G13450.1.

PTM databases

iPTMnetiQ9FX53.

Proteomic databases

PaxDbiQ9FX53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G13450.1; AT1G13450.1; AT1G13450. [Q9FX53-1]
GeneIDi837905.
GrameneiAT1G13450.1; AT1G13450.1; AT1G13450.
KEGGiath:AT1G13450.

Organism-specific databases

TAIRiAT1G13450.

Phylogenomic databases

eggNOGiENOG410IN2M. Eukaryota.
ENOG410XUBM. LUCA.
HOGENOMiHOG000064692.
InParanoidiQ9FX53.
OMAiFKKAKHH.
OrthoDBiEOG09360IMV.
PhylomeDBiQ9FX53.

Miscellaneous databases

EvolutionaryTraceiQ9FX53.
PROiQ9FX53.

Gene expression databases

GenevisibleiQ9FX53. AT.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR027775. C2H2_Znf_fam.
IPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
PROSITEiPS50090. MYB_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGT1_ARATH
AccessioniPrimary (citable) accession number: Q9FX53
Secondary accession number(s): B3H720, Q2L6T7, Q39116
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.