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Protein

Mitogen-activated protein kinase kinase 9

Gene

MKK9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. Autophosphorylates and also phosphorylates MPK3 and MPK6. Plays an important role in ethylene and camalexin biosynthesis and in salt stress response. MKK9-MPK6 module positively regulates leaf senescence.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761ATPPROSITE-ProRule annotation
Active sitei167 – 1671Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 619ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase activity Source: TAIR
  • protein kinase activator activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • ethylene biosynthetic process Source: TAIR
  • leaf senescence Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: TAIR
  • protein autophosphorylation Source: TAIR
  • response to fungus Source: UniProtKB
  • response to salt stress Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Ethylene biosynthesis, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G73500-MONOMER.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 9 (EC:2.7.12.2)
Short name:
AtMKK9
Short name:
MAP kinase kinase 9
Gene namesi
Name:MKK9
Synonyms:MAP2K9
Ordered Locus Names:At1g73500
ORF Names:T9L24.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G73500.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Translocated into the nucleus by ACC treatment.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Broad spectrum of moderate ethylene-insensitive phenotypes. Reduced salt sensitivity. Delayed senescence phenotype.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi76 – 761K → R: Abolishes kinase activity. 2 Publications
Mutagenesisi195 – 1951S → E: Constitutively active; when associated with E-201. 1 Publication
Mutagenesisi201 – 2011S → D: Constitutively active; when associated with D-205. 2 Publications
Mutagenesisi201 – 2011S → E: Constitutively active; when associated with E-195. 2 Publications
Mutagenesisi205 – 2051T → D: Constitutively active; when associated with D-201. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Mitogen-activated protein kinase kinase 9PRO_0000428625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951Phosphoserine1 Publication
Modified residuei201 – 2011Phosphoserine2 Publications
Modified residuei205 – 2051Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation at Ser-195 and Ser-201 by MAP kinase kinase kinases positively regulates kinase activity. Autophosphorylated (Probable).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FX43.
PRIDEiQ9FX43.

PTM databases

iPTMnetiQ9FX43.

Expressioni

Inductioni

Up-regulated during leaf senescence. By Alternaria brassicae pathogen infection.2 Publications

Gene expression databases

GenevisibleiQ9FX43. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK10Q9M1Z52EBI-2128545,EBI-2358527
MPK12Q8GYQ52EBI-2128545,EBI-2128461
MPK20Q9SJG92EBI-2128545,EBI-2358896

Protein-protein interaction databases

BioGridi28904. 4 interactions.
IntActiQ9FX43. 8 interactions.
STRINGi3702.AT1G73500.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FX43.
SMRiQ9FX43. Positions 46-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 306260Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi25 – 306Poly-Ser

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9FX43.
OMAiHPFLRED.
OrthoDBiEOG09360IH3.
PhylomeDBiQ9FX43.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FX43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVRERRQL NLRLPLPPIS DRRFSTSSSS ATTTTVAGCN GISACDLEKL
60 70 80 90 100
NVLGCGNGGI VYKVRHKTTS EIYALKTVNG DMDPIFTRQL MREMEILRRT
110 120 130 140 150
DSPYVVKCHG IFEKPVVGEV SILMEYMDGG TLESLRGGVT EQKLAGFAKQ
160 170 180 190 200
ILKGLSYLHA LKIVHRDIKP ANLLLNSKNE VKIADFGVSK ILVRSLDSCN
210 220 230 240 250
SYVGTCAYMS PERFDSESSG GSSDIYAGDI WSFGLMMLEL LVGHFPLLPP
260 270 280 290 300
GQRPDWATLM CAVCFGEPPR APEGCSEEFR SFVECCLRKD SSKRWTAPQL
310
LAHPFLREDL
Length:310
Mass (Da):34,347
Last modified:March 1, 2001 - v1
Checksum:iCF855943643D9261
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012396 Genomic DNA. Translation: AAG30984.1.
CP002684 Genomic DNA. Translation: AEE35470.1.
AK118530 mRNA. Translation: BAC43133.1.
BT005300 mRNA. Translation: AAO63364.1.
AY084571 mRNA. Translation: AAM61137.1.
PIRiG96761.
RefSeqiNP_177492.1. NM_106009.2.
UniGeneiAt.27674.
At.67792.

Genome annotation databases

EnsemblPlantsiAT1G73500.1; AT1G73500.1; AT1G73500.
GeneIDi843685.
GrameneiAT1G73500.1; AT1G73500.1; AT1G73500.
KEGGiath:AT1G73500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012396 Genomic DNA. Translation: AAG30984.1.
CP002684 Genomic DNA. Translation: AEE35470.1.
AK118530 mRNA. Translation: BAC43133.1.
BT005300 mRNA. Translation: AAO63364.1.
AY084571 mRNA. Translation: AAM61137.1.
PIRiG96761.
RefSeqiNP_177492.1. NM_106009.2.
UniGeneiAt.27674.
At.67792.

3D structure databases

ProteinModelPortaliQ9FX43.
SMRiQ9FX43. Positions 46-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28904. 4 interactions.
IntActiQ9FX43. 8 interactions.
STRINGi3702.AT1G73500.1.

PTM databases

iPTMnetiQ9FX43.

Proteomic databases

PaxDbiQ9FX43.
PRIDEiQ9FX43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G73500.1; AT1G73500.1; AT1G73500.
GeneIDi843685.
GrameneiAT1G73500.1; AT1G73500.1; AT1G73500.
KEGGiath:AT1G73500.

Organism-specific databases

TAIRiAT1G73500.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9FX43.
OMAiHPFLRED.
OrthoDBiEOG09360IH3.
PhylomeDBiQ9FX43.

Enzyme and pathway databases

BioCyciARA:AT1G73500-MONOMER.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiQ9FX43.

Gene expression databases

GenevisibleiQ9FX43. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM2K9_ARATH
AccessioniPrimary (citable) accession number: Q9FX43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.