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Protein

Mitogen-activated protein kinase kinase 9

Gene

MKK9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. Autophosphorylates and also phosphorylates MPK3 and MPK6. Plays an important role in ethylene and camalexin biosynthesis and in salt stress response. MKK9-MPK6 module positively regulates leaf senescence.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 61ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase activity Source: TAIR
  • protein kinase activator activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • ethylene biosynthetic process Source: TAIR
  • leaf senescence Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: TAIR
  • protein autophosphorylation Source: TAIR
  • regulation of mitotic cell cycle Source: GO_Central
  • response to fungus Source: UniProtKB
  • response to salt stress Source: TAIR
  • response to wounding Source: TAIR
  • stress-activated protein kinase signaling cascade Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processEthylene biosynthesis, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 9 (EC:2.7.12.2)
Short name:
AtMKK9
Short name:
MAP kinase kinase 9
Gene namesi
Name:MKK9
Synonyms:MAP2K9
Ordered Locus Names:At1g73500
ORF Names:T9L24.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G73500.
TAIRilocus:2206885. AT1G73500.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Broad spectrum of moderate ethylene-insensitive phenotypes. Reduced salt sensitivity. Delayed senescence phenotype.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76K → R: Abolishes kinase activity. 2 Publications1
Mutagenesisi195S → E: Constitutively active; when associated with E-201. 1 Publication1
Mutagenesisi201S → D: Constitutively active; when associated with D-205. 2 Publications1
Mutagenesisi201S → E: Constitutively active; when associated with E-195. 2 Publications1
Mutagenesisi205T → D: Constitutively active; when associated with D-201. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004286251 – 310Mitogen-activated protein kinase kinase 9Add BLAST310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195Phosphoserine1 Publication1
Modified residuei201Phosphoserine2 Publications1
Modified residuei205Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylation at Ser-195 and Ser-201 by MAP kinase kinase kinases positively regulates kinase activity. Autophosphorylated (Probable).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FX43.

PTM databases

iPTMnetiQ9FX43.

Expressioni

Inductioni

Up-regulated during leaf senescence. By Alternaria brassicae pathogen infection.2 Publications

Gene expression databases

ExpressionAtlasiQ9FX43. baseline and differential.
GenevisibleiQ9FX43. AT.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi28904. 5 interactors.
IntActiQ9FX43. 8 interactors.
STRINGi3702.AT1G73500.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FX43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 306Protein kinasePROSITE-ProRule annotationAdd BLAST260

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 30Poly-Ser6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9FX43.
KOiK20604.
OMAiINDPPPR.
OrthoDBiEOG09360IH3.
PhylomeDBiQ9FX43.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FX43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVRERRQL NLRLPLPPIS DRRFSTSSSS ATTTTVAGCN GISACDLEKL
60 70 80 90 100
NVLGCGNGGI VYKVRHKTTS EIYALKTVNG DMDPIFTRQL MREMEILRRT
110 120 130 140 150
DSPYVVKCHG IFEKPVVGEV SILMEYMDGG TLESLRGGVT EQKLAGFAKQ
160 170 180 190 200
ILKGLSYLHA LKIVHRDIKP ANLLLNSKNE VKIADFGVSK ILVRSLDSCN
210 220 230 240 250
SYVGTCAYMS PERFDSESSG GSSDIYAGDI WSFGLMMLEL LVGHFPLLPP
260 270 280 290 300
GQRPDWATLM CAVCFGEPPR APEGCSEEFR SFVECCLRKD SSKRWTAPQL
310
LAHPFLREDL
Length:310
Mass (Da):34,347
Last modified:March 1, 2001 - v1
Checksum:iCF855943643D9261
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012396 Genomic DNA. Translation: AAG30984.1.
CP002684 Genomic DNA. Translation: AEE35470.1.
AK118530 mRNA. Translation: BAC43133.1.
BT005300 mRNA. Translation: AAO63364.1.
AY084571 mRNA. Translation: AAM61137.1.
PIRiG96761.
RefSeqiNP_177492.1. NM_106009.3.
UniGeneiAt.27674.
At.67792.

Genome annotation databases

EnsemblPlantsiAT1G73500.1; AT1G73500.1; AT1G73500.
GeneIDi843685.
GrameneiAT1G73500.1; AT1G73500.1; AT1G73500.
KEGGiath:AT1G73500.

Similar proteinsi

Entry informationi

Entry nameiM2K9_ARATH
AccessioniPrimary (citable) accession number: Q9FX43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: March 1, 2001
Last modified: November 22, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families