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Q9FX32

- SUS6_ARATH

UniProt

Q9FX32 - SUS6_ARATH

Protein

Sucrose synthase 6

Gene

SUS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Functions in callose synthesis at the site of phloem sieve elements.1 Publication

    Catalytic activityi

    NDP-glucose + D-fructose = NDP + sucrose.

    Kineticsi

    1. KM=12.44 mM for D-fructose (synthetic reaction)1 Publication
    2. KM=0.06 mM for UDP-glucose (synthetic reaction)1 Publication
    3. KM=31.06 mM for sucrose (degradative reaction)1 Publication
    4. KM=0.26 mM for UDP (degradative reaction)1 Publication

    Vmax=3.27 µmol/min/mg enzyme for synthetic reaction1 Publication

    Vmax=1.43 µmol/min/mg enzyme for degradative reaction1 Publication

    pH dependencei

    Optimum pH is 6.0-7.0 for degradative reaction and 7.0 (PubMed:22184213) or 9.0-9.5 (PubMed:17257168) for synthetic reaction.1 Publication

    GO - Molecular functioni

    1. sucrose synthase activity Source: TAIR

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. callose deposition in phloem sieve plate Source: TAIR
    3. sucrose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:GQT-78-MONOMER.
    MetaCyc:AT1G73370-MONOMER.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrose synthase 6 (EC:2.4.1.13)
    Short name:
    AtSUS6
    Alternative name(s):
    Sucrose-UDP glucosyltransferase 6
    Gene namesi
    Name:SUS6
    Ordered Locus Names:At1g73370
    ORF Names:T9L24.42
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G73370.

    Subcellular locationi

    Secretedcell wall 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Sucrose synthase 6PRO_0000418805Add
    BLAST

    Proteomic databases

    PaxDbiQ9FX32.
    PRIDEiQ9FX32.

    Expressioni

    Tissue specificityi

    Detected in the whole plant but more precisely confined to the vasculature in cotyledons, leaves, petals, anthers and roots.4 Publications

    Gene expression databases

    GenevestigatoriQ9FX32.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G73370.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FX32.
    SMRiQ9FX32. Positions 41-812.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni281 – 759479GT-B glycosyltransferaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0438.
    HOGENOMiHOG000240125.
    InParanoidiQ9FX32.
    KOiK00695.
    OMAiFFEKCKT.
    PhylomeDBiQ9FX32.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FX32-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSSSQAMLQ KSDSIAEKMP DALKQSRYHM KRCFASFVGG GKKLMKREHL    50
    MNEIEKCIED SRERSKILEG LFGYILTCTQ EAAVVPPFVA LAARPNPGFW 100
    EYVKVNSGDL TVDEITATDY LKLKESVFDE SWSKDENALE IDFGAIDFTS 150
    PRLSLSSSIG KGADYISKFI SSKLGGKSDK LEPLLNYLLR LNHHGENLMI 200
    NDDLNTVAKL QKSLMLAVIV VSTYSKHTPY ETFAQRLKEM GFEKGWGDTA 250
    ERVKETMIIL SEVLEAPDNG KLDLLFSRLP TVFNVVIFSV HGYFGQQDVL 300
    GLPDTGGQVV YILDQVRALE EELLIRINQQ GLGFKPQILV VTRLIPEARG 350
    TKCDQELEAI EGTKHSHILR VPFVTNKGVL RQWVSRFDIY PYLERFTQDA 400
    TSKILQRLDC KPDLIIGNYT DGNLVASLMA TKLGVTQGTI AHALEKTKYE 450
    DSDAKWKELD PKYHFSCQFT ADLIAMNVTD FIITSTYQEI AGSKDRPGQY 500
    ESHTAFTMPG LCRVVSGIDV FDPKFNIAAP GADQSVYFPY TEKDKRFTKF 550
    HPSIQELLYN EKDNAEHMGY LADREKPIIF SMARLDTVKN ITGLVEWYGK 600
    DKRLREMANL VVVAGFFDMS KSNDREEKAE IKKMHDLIEK YKLKGKFRWI 650
    AAQTDRYRNS ELYRCIADTK GVFVQPALYE AFGLTVIEAM NCGLPTFATN 700
    QGGPAEIIVD GVSGFHIDPN NGDESVTKIG DFFSKCRSDG LYWDNISKGG 750
    LKRIYECYTW KIYAEKLLKM GSLYGFWRQV NEDQKKAKKR YIEMLYNLQF 800
    KQLTKKVTIP EDKPLPLRLA SLRNLLPKKT TNLGAGSKQK EVTETEKTKQ 850
    KSKDGQEQHD VKVGEREVRE GLLAADASER VKKVLESSEE KQKLEKMKIA 900
    YGQQHSQGAS PVRNLFWSVV VCLYICYILK QRFFGANSAQ EY 942
    Length:942
    Mass (Da):106,876
    Last modified:March 1, 2001 - v1
    Checksum:i0AA4A92EC48815EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012396 Genomic DNA. Translation: AAG30975.1.
    CP002684 Genomic DNA. Translation: AEE35450.1.
    PIRiC96760.
    RefSeqiNP_177480.1. NM_105997.1. [Q9FX32-1]
    UniGeneiAt.34995.
    At.69495.

    Genome annotation databases

    EnsemblPlantsiAT1G73370.1; AT1G73370.1; AT1G73370. [Q9FX32-1]
    GeneIDi843672.
    KEGGiath:AT1G73370.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012396 Genomic DNA. Translation: AAG30975.1 .
    CP002684 Genomic DNA. Translation: AEE35450.1 .
    PIRi C96760.
    RefSeqi NP_177480.1. NM_105997.1. [Q9FX32-1 ]
    UniGenei At.34995.
    At.69495.

    3D structure databases

    ProteinModelPortali Q9FX32.
    SMRi Q9FX32. Positions 41-812.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G73370.1-P.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    Proteomic databases

    PaxDbi Q9FX32.
    PRIDEi Q9FX32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G73370.1 ; AT1G73370.1 ; AT1G73370 . [Q9FX32-1 ]
    GeneIDi 843672.
    KEGGi ath:AT1G73370.

    Organism-specific databases

    TAIRi AT1G73370.

    Phylogenomic databases

    eggNOGi COG0438.
    HOGENOMi HOG000240125.
    InParanoidi Q9FX32.
    KOi K00695.
    OMAi FFEKCKT.
    PhylomeDBi Q9FX32.

    Enzyme and pathway databases

    BioCyci ARA:GQT-78-MONOMER.
    MetaCyc:AT1G73370-MONOMER.

    Gene expression databases

    Genevestigatori Q9FX32.

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view ]
    Pfami PF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02470. sucr_synth. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Structure and expression profile of the sucrose synthase multigene family in Arabidopsis."
      Baud S., Vaultier M.N., Rochat C.
      J. Exp. Bot. 55:397-409(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, TISSUE SPECIFICITY.
    4. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    5. "Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development."
      Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.
      J. Exp. Bot. 59:3283-3295(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.

    Entry informationi

    Entry nameiSUS6_ARATH
    AccessioniPrimary (citable) accession number: Q9FX32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3