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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Gene

STT3B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity (By similarity).By similarity1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G34130-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit STT3B
Short name:
STT3-B
Alternative name(s):
Protein STAUROSPORIN AND TEMPERATURE SENSITIVE 3-LIKE B
Gene namesi
Name:STT3B
Ordered Locus Names:At1g34130
ORF Names:F12G12.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G34130.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence analysisAdd
BLAST
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Topological domaini60 – 13778LumenalSequence analysisAdd
BLAST
Transmembranei138 – 15821HelicalSequence analysisAdd
BLAST
Topological domaini159 – 19234CytoplasmicSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Topological domaini214 – 23017LumenalSequence analysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence analysisAdd
BLAST
Topological domaini252 – 2609CytoplasmicSequence analysis
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Topological domaini282 – 2887LumenalSequence analysis
Transmembranei289 – 30921HelicalSequence analysisAdd
BLAST
Topological domaini310 – 32617CytoplasmicSequence analysisAdd
BLAST
Transmembranei327 – 34721HelicalSequence analysisAdd
BLAST
Topological domaini348 – 38235LumenalSequence analysisAdd
BLAST
Transmembranei383 – 40321HelicalSequence analysisAdd
BLAST
Topological domaini404 – 4074CytoplasmicSequence analysis
Transmembranei408 – 42821HelicalSequence analysisAdd
BLAST
Topological domaini429 – 4291LumenalSequence analysis
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Topological domaini451 – 49444CytoplasmicSequence analysisAdd
BLAST
Transmembranei495 – 51521HelicalSequence analysisAdd
BLAST
Topological domaini516 – 735220LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • oligosaccharyltransferase complex Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3BPRO_0000420537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi574 – 5741N-linked (GlcNAc...)Sequence analysis
Glycosylationi581 – 5811N-linked (GlcNAc...)Sequence analysis
Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FX21.
PRIDEiQ9FX21.

PTM databases

SwissPalmiQ9FX21.

Expressioni

Tissue specificityi

Expressed preferentially in the root but also in the shoot.1 Publication

Gene expression databases

ExpressionAtlasiQ9FX21. baseline and differential.
GenevisibleiQ9FX21. AT.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G34130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FX21.
SMRiQ9FX21. Positions 511-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STT3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
InParanoidiQ9FX21.
KOiK07151.
OMAiVSESICM.
OrthoDBiEOG093603HW.
PhylomeDBiQ9FX21.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FX21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGKSEPAKS ESMATKPDLL NTSFFSFKSL KLKTKQQELL LRISILGLVY
60 70 80 90 100
ILAFIARLFS VLRYESMIHE FDPYFNYRTT LFLTEKGFYE FWNWFDSESW
110 120 130 140 150
YPLGRIIGGT LYPGLMVTAA LIYWTLRFLR FFVHIREVCV LTAPFFASNT
160 170 180 190 200
TLVAYFFGKE LWDTGAGLVA AVLIAICPGY ISRSVAGSYD NEAVAIFALL
210 220 230 240 250
LTFYLFVKAV NTGSLAWALA SAFGYFYMVS AWGGYVFIIN LVPLYVLVLL
260 270 280 290 300
ITGRYSMRLY IAYNCMYILG MLLAMQIRFV GFQHVQSGEH MGAMGVFLLM
310 320 330 340 350
QVFYFLDWVK YQLNDTKLFQ TFLRITVTSA ILVGGVAVGV GTASGYISPW
360 370 380 390 400
TGRFYSLLDP TYAKDHIPII ASVSEHQPTA WSSFMFDYHI LLFLFPAGLY
410 420 430 440 450
FCFKRLTDAT IFIVMYGLTS LYFAGVMVRL ILVATPAVCL ISAIAVSATI
460 470 480 490 500
KNLTSLLRTK QKVSQTGSTK GAGSSKASSK VTLDQSQPFQ KNGAIALLVG
510 520 530 540 550
VFYLLSRYAI HCTWVTAEAY SSPSIVLAAR GAHGNRIIFD DYREAYYWLR
560 570 580 590 600
QNTATDAKIM SWWDYGYQIT AMGNRTVIVD NNTWNNTHIA TVGRAMSSYE
610 620 630 640 650
DDAYDIMRSL DVNYVLVVFG GVTGYSSDDI NKFLWMVRIG GGVFPVIKEP
660 670 680 690 700
DYLVNGEFRV DKGASPKMLN CLMYKLCYYR FGELTTEYGK PPGYDRARGV
710 720 730
EIGNKDIKLE HLEEAYTTSN WIVRIYRVKP PTNRL
Length:735
Mass (Da):82,980
Last modified:March 1, 2001 - v1
Checksum:iDBBB06AD4D97CAB4
GO

Sequence cautioni

The sequence BX816490 differs from that shown. Reason: Frameshift at positions 280, 283 and 286. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti595 – 5951A → P in BX816490 (PubMed:14993207).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC015446 Genomic DNA. Translation: AAG12524.1.
CP002684 Genomic DNA. Translation: AEE31675.1.
BX816490 mRNA. No translation available.
AK229582 mRNA. Translation: BAF01432.1.
PIRiD86465.
RefSeqiNP_174675.2. NM_103136.3.
UniGeneiAt.43610.

Genome annotation databases

EnsemblPlantsiAT1G34130.1; AT1G34130.1; AT1G34130.
GeneIDi840312.
GrameneiAT1G34130.1; AT1G34130.1; AT1G34130.
KEGGiath:AT1G34130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC015446 Genomic DNA. Translation: AAG12524.1.
CP002684 Genomic DNA. Translation: AEE31675.1.
BX816490 mRNA. No translation available.
AK229582 mRNA. Translation: BAF01432.1.
PIRiD86465.
RefSeqiNP_174675.2. NM_103136.3.
UniGeneiAt.43610.

3D structure databases

ProteinModelPortaliQ9FX21.
SMRiQ9FX21. Positions 511-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G34130.1.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

PTM databases

SwissPalmiQ9FX21.

Proteomic databases

PaxDbiQ9FX21.
PRIDEiQ9FX21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G34130.1; AT1G34130.1; AT1G34130.
GeneIDi840312.
GrameneiAT1G34130.1; AT1G34130.1; AT1G34130.
KEGGiath:AT1G34130.

Organism-specific databases

TAIRiAT1G34130.

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
InParanoidiQ9FX21.
KOiK07151.
OMAiVSESICM.
OrthoDBiEOG093603HW.
PhylomeDBiQ9FX21.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT1G34130-MONOMER.

Miscellaneous databases

PROiQ9FX21.

Gene expression databases

ExpressionAtlasiQ9FX21. baseline and differential.
GenevisibleiQ9FX21. AT.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTT3B_ARATH
AccessioniPrimary (citable) accession number: Q9FX21
Secondary accession number(s): Q0WN68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.