Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1

Gene

3BETAHSD/D1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Delta(14)-sterol reductase (FK)
  3. no protein annotated in this organism
  4. 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 (3BETAHSD/D3), 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (3BETAHSD/D1), 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (3BETAHSD/D2)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Proton acceptorBy similarity1
Binding sitei165NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT1G47290-MONOMER.
MetaCyc:AT1G47290-MONOMER.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (EC:1.1.1.170)
Short name:
At3BETAHSD/D1
Alternative name(s):
4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1
Reticulon-like protein B24
Short name:
AtRTNLB24
Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating
Gene namesi
Name:3BETAHSD/D1
Synonyms:RTNLB24
Ordered Locus Names:At1g47290
ORF Names:T3F24.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G47290.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003712791 – 4393beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1Add BLAST439

Proteomic databases

PaxDbiQ9FX01.

Expressioni

Gene expression databases

GenevisibleiQ9FX01. AT.

Interactioni

Protein-protein interaction databases

BioGridi26357. 2 interactors.
IntActiQ9FX01. 2 interactors.
STRINGi3702.AT1G47290.2.

Structurei

3D structure databases

ProteinModelPortaliQ9FX01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini371 – 439Reticulon; atypicalPROSITE-ProRule annotationAdd BLAST69

Domaini

The RETICULON domain is partial in comparison with the other paralogs.

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated
Contains 1 reticulon domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000167989.
InParanoidiQ9FX01.
KOiK07748.
OMAiESLPYPP.
OrthoDBiEOG093609X1.
PhylomeDBiQ9FX01.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FX01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMEVTETER WCVVTGGRGF AARHLVEMLV RYQMFHVRIA DLAPAIVLNP
60 70 80 90 100
HEETGILGEA IRSGRVQYVS ADLRNKTQVV KGFQGAEVVF HMAAPDSSIN
110 120 130 140 150
NHQLQYSVNV QGTTNVIDAC IEVGVKRLIY TSSPSVVFDG VHGTLNADES
160 170 180 190 200
LPYPPKHNDS YSATKAEGEA LILKANGRSG LLTCCIRPSS IFGPGDKLMV
210 220 230 240 250
PSLVTAARAG KSKFIIGDGS NFYDFTYVEN VVHAHVCAER ALASGGEVCA
260 270 280 290 300
KAAGQAYFIT NMEPIKFWEF MSQLLEGLGY ERPSIKIPAS LMMPIAYLVE
310 320 330 340 350
LAYKLLGPYG MKVPVLTPSR VRLLSCNRTF DSSKAKDRLG YSPVVPLQEG
360 370 380 390 400
IKRTIDSFSH LKAQNQPKTE VTETIQWKKQ TLIAIVILIT LYHNFVATTG
410 420 430
SSSVIITAVS KVLLVSSIFM FINGILPEKM KVFGSKKID
Length:439
Mass (Da):48,125
Last modified:March 1, 2001 - v1
Checksum:iC976A205FAFD87ED
GO
Isoform 2 (identifier: Q9FX01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     371-427: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.Curated
Show »
Length:382
Mass (Da):41,908
Checksum:iD24CD4F98A029441
GO

Sequence cautioni

The sequence AAM62504 differs from that shown. Reason: Erroneous initiation.Curated
Isoform 2 : The sequence AAM62504 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAY28502 differs from that shown. Reason: Erroneous initiation.Curated
Isoform 2 : The sequence AAY28502 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49N → D in AAM62504 (Ref. 6) Curated1
Sequence conflicti70S → P in AAY28502 (PubMed:16835224).Curated1
Sequence conflicti228V → F in AAM62504 (Ref. 6) Curated1
Sequence conflicti336K → R in AAY28502 (PubMed:16835224).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037002371 – 427Missing in isoform 2. 2 PublicationsAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957470 mRNA. Translation: AAY28502.1. Different initiation.
AC015449 Genomic DNA. Translation: AAG11424.1.
CP002684 Genomic DNA. Translation: AEE32152.1.
CP002684 Genomic DNA. Translation: AEE32153.1.
AK117478 mRNA. Translation: BAC42142.1.
BT005166 mRNA. Translation: AAO50699.1.
AY085272 mRNA. Translation: AAM62504.1. Different initiation.
PIRiF96513.
RefSeqiNP_564502.1. NM_103623.5. [Q9FX01-2]
NP_849779.1. NM_179448.4. [Q9FX01-1]
UniGeneiAt.38654.

Genome annotation databases

EnsemblPlantsiAT1G47290.2; AT1G47290.2; AT1G47290. [Q9FX01-1]
GeneIDi841132.
GrameneiAT1G47290.2; AT1G47290.2; AT1G47290.
KEGGiath:AT1G47290.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY957470 mRNA. Translation: AAY28502.1. Different initiation.
AC015449 Genomic DNA. Translation: AAG11424.1.
CP002684 Genomic DNA. Translation: AEE32152.1.
CP002684 Genomic DNA. Translation: AEE32153.1.
AK117478 mRNA. Translation: BAC42142.1.
BT005166 mRNA. Translation: AAO50699.1.
AY085272 mRNA. Translation: AAM62504.1. Different initiation.
PIRiF96513.
RefSeqiNP_564502.1. NM_103623.5. [Q9FX01-2]
NP_849779.1. NM_179448.4. [Q9FX01-1]
UniGeneiAt.38654.

3D structure databases

ProteinModelPortaliQ9FX01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26357. 2 interactors.
IntActiQ9FX01. 2 interactors.
STRINGi3702.AT1G47290.2.

Proteomic databases

PaxDbiQ9FX01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G47290.2; AT1G47290.2; AT1G47290. [Q9FX01-1]
GeneIDi841132.
GrameneiAT1G47290.2; AT1G47290.2; AT1G47290.
KEGGiath:AT1G47290.

Organism-specific databases

TAIRiAT1G47290.

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
HOGENOMiHOG000167989.
InParanoidiQ9FX01.
KOiK07748.
OMAiESLPYPP.
OrthoDBiEOG093609X1.
PhylomeDBiQ9FX01.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00757.
BioCyciARA:AT1G47290-MONOMER.
MetaCyc:AT1G47290-MONOMER.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiQ9FX01.

Gene expression databases

GenevisibleiQ9FX01. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHSDD1_ARATH
AccessioniPrimary (citable) accession number: Q9FX01
Secondary accession number(s): Q0VH36, Q8LER8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Lacks one transmembrane, which is a conserved feature of the family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.