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Q9FWX8 (AB12B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ABC transporter B family member 12
Short name=ABC transporter ABCB.12
Short name=AtABCB12
Alternative name(s):
Multidrug resistance protein 16
P-glycoprotein 12
Gene names
Name:ABCB12
Synonyms:MDR16, PGP12
Ordered Locus Names:At1g02530
ORF Names:T14P4.14
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1273 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence caution

The sequence AAG10627.1 differs from that shown. Reason: Erroneous initiation.

The sequence AK228647 differs from that shown. Reason: Frameshift at positions 105 and 420.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity, coupled to transmembrane movement of substances

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12731273ABC transporter B family member 12
PRO_0000227927

Regions

Transmembrane42 – 6221Helical; Potential
Transmembrane93 – 11321Helical; Potential
Transmembrane169 – 18921Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane272 – 29221Helical; Potential
Transmembrane301 – 32121Helical; Potential
Transmembrane706 – 72621Helical; Potential
Transmembrane746 – 76621Helical; Potential
Transmembrane840 – 86021Helical; Potential
Transmembrane927 – 94721Helical; Potential
Transmembrane961 – 98121Helical; Potential
Domain45 – 333289ABC transmembrane type-1 1
Domain368 – 604237ABC transporter 1
Domain705 – 992288ABC transmembrane type-1 2
Domain1027 – 1266240ABC transporter 2
Nucleotide binding403 – 4108ATP 1 Potential
Nucleotide binding1062 – 10698ATP 2 Potential

Amino acid modifications

Glycosylation4701N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential
Glycosylation6311N-linked (GlcNAc...) Potential
Glycosylation6481N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential
Glycosylation11161N-linked (GlcNAc...) Potential
Glycosylation12171N-linked (GlcNAc...) Potential
Glycosylation12471N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2791I → T in AK228647. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9FWX8 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 1CCA06FB3D472241

FASTA1,273136,774
        10         20         30         40         50         60 
MNRDGAGEGD SVSHEHSTSK TDEKAKTVPL YKLFAFADSF DVFLMICGSL GAIGNGVCLP 

        70         80         90        100        110        120 
LMTLLFGDLI DSFGKNQNNK DIVDVVSKVC LKFVYLGLGR LGAAFLQVAC WMITGERQAA 

       130        140        150        160        170        180 
KIRSNYLKTI LRQDIGFFDV ETNTGEVVGR MSGDTVHIQD AMGEKVGKFI QLVSTFVGGF 

       190        200        210        220        230        240 
ALAFAKGWLL TLVMLTSIPF LAMAGAAMAL LVTRASSRGQ AAYAKAATVV EQTIGSIRTV 

       250        260        270        280        290        300 
ASFTGEKQAI NSYKKYITSA YKSSIQQGFS TGLGLGVMIY VFFSSYALAI WFGGKMILEK 

       310        320        330        340        350        360 
GYTGGSVINV IIIVVAGSMS LGQTSPCVTA FAAGQAAAYK MFETIKRKPL IDAYDVNGKV 

       370        380        390        400        410        420 
LGDIRGDIEL KDVHFSYPAR PDEEIFDGFS LFIPSGATAA LVGESGSGKS TVINLIERFY 

       430        440        450        460        470        480 
DPKAGEVLID GINLKEFQLK WIRSKIGLVC QEPVLFSSSI MENIAYGKEN ATLQEIKVAT 

       490        500        510        520        530        540 
ELANAAKFIN NLPQGLDTKV GEHGTQLSGG QKQRIAIARA ILKDPRVLLL DEATSALDTE 

       550        560        570        580        590        600 
SERVVQEALD RVMVNRTTVV VAHRLSTVRN ADMIAVIHSG KMVEKGSHSE LLKDSVGAYS 

       610        620        630        640        650        660 
QLIRCQEINK GHDAKPSDMA SGSSFRNSNL NISREGSVIS GGTSSFGNSS RHHSLNVLGL 

       670        680        690        700        710        720 
FAGLDLGSGS QRVGQEETGT TSQEPLRKVS LTRIAALNKP EIPVLLLGTV VAAINGAIFP 

       730        740        750        760        770        780 
LFGILISRVI EAFFKPADQL KKDSRFWAII FVALGVTSLI VSPSQMYLFA VAGGKLIRRI 

       790        800        810        820        830        840 
QSMCFEKAVH MEVSWFDEPE NSSGTMGARL STDAALIRAL VGDALSLAVQ NAASAASGLI 

       850        860        870        880        890        900 
IAFTASWELA LIILVMLPLI GINGFLQVKF MKGFSADAKS KYEEASQVAN DAVGSIRTVA 

       910        920        930        940        950        960 
SFCAEEKVMQ MYNKQCEGPI KDGVKQGFIS GLGFGFSFFI LFCVYATSFY AAARLVEDGK 

       970        980        990       1000       1010       1020 
TTFIDVFQVF FALTMAAIGI SQSSTFAPDS SKAKVAAASI FAIIDRKSKI DSSDETGTVL 

      1030       1040       1050       1060       1070       1080 
ENVKGDIELR HLSFTYPARP GIQIFRDLCL TIRAGKTVAL VGESGSGKST VISLLQRFYD 

      1090       1100       1110       1120       1130       1140 
PDSGQITLDG VELKKLQLKW LRQQMGLVGQ EPVLFNDTIR ANIAYGKGSE EAATESEIIA 

      1150       1160       1170       1180       1190       1200 
AAELANAHKF ISSIQQGYDT VVGEKGIQLS GGQKQRVAIA RAIVKEPKIL LLDEATSALD 

      1210       1220       1230       1240       1250       1260 
AESERLVQDA LDRVIVNRTT VVVAHRLSTI KNADVIAIVK NGVIAENGTH ETLIKIDGGV 

      1270 
YASLVQLHMT ASN

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The Arabidopsis thaliana ABC protein superfamily, a complete inventory."
Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.
J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Plant ABC proteins - a unified nomenclature and updated inventory."
Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.
Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC022521 Genomic DNA. Translation: AAG10627.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE27441.1.
AK228647 mRNA. No translation available.
IPIIPI00526168.
PIRF86155.
RefSeqNP_171754.1. NM_100134.3.
UniGeneAt.42595.

3D structure databases

ProteinModelPortalQ9FWX8.
SMRQ9FWX8. Positions 28-607, 690-1272.
ModBaseSearch...

Proteomic databases

PRIDEQ9FWX8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G02530.1; AT1G02530.1; AT1G02530.
GeneID839282.
GenomeReviewsGene locus AT1G02530 in contig CT485782_GR.
KEGGath:AT1G02530.
NMPDRfig|3702.1.peg.374.

Organism-specific databases

TAIRAt1g02530.

Phylogenomic databases

eggNOGKOG0055.
GeneTreeEPGT00070000028344.
HOGENOMHBG758042.
InParanoidQ9FWX8.
OMAENGTHET.
PhylomeDBQ9FWX8.
ProtClustDBCLSN2679354.

Gene expression databases

GenevestigatorQ9FWX8.
GermOnlineAT1G02530. Arabidopsis thaliana.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
KOK05658.
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAB12B_ARATH
AccessionPrimary (citable) accession number: Q9FWX8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 14, 2008
Last modified: December 14, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents