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Protein

ABC transporter B family member 12

Gene

ABCB12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi403 – 410ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1062 – 1069ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G02530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter B family member 12
Short name:
ABC transporter ABCB.12
Short name:
AtABCB12
Alternative name(s):
Multidrug resistance protein 16
P-glycoprotein 12
Gene namesi
Name:ABCB12
Synonyms:MDR16, PGP12
Ordered Locus Names:At1g02530
ORF Names:T14P4.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02530.

Subcellular locationi

  • Membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei42 – 62HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei93 – 113HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei169 – 189HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei192 – 212HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei272 – 292HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei301 – 321HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei706 – 726HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei746 – 766HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei840 – 860HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei927 – 947HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei961 – 981HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002279271 – 1273ABC transporter B family member 12Add BLAST1273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi470N-linked (GlcNAc...)Sequence analysis1
Glycosylationi555N-linked (GlcNAc...)Sequence analysis1
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Glycosylationi648N-linked (GlcNAc...)Sequence analysis1
Glycosylationi801N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1116N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1217N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1247N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FWX8.
PRIDEiQ9FWX8.

Expressioni

Gene expression databases

GenevisibleiQ9FWX8. AT.

Interactioni

Protein-protein interaction databases

BioGridi24517. 1 interactor.
STRINGi3702.AT1G02530.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FWX8.
SMRiQ9FWX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 333ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST289
Domaini368 – 604ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini705 – 992ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST288
Domaini1027 – 1266ABC transporter 2PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9FWX8.
KOiK05658.
OMAiTIIFADI.
OrthoDBiEOG093600JM.
PhylomeDBiQ9FWX8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FWX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRDGAGEGD SVSHEHSTSK TDEKAKTVPL YKLFAFADSF DVFLMICGSL
60 70 80 90 100
GAIGNGVCLP LMTLLFGDLI DSFGKNQNNK DIVDVVSKVC LKFVYLGLGR
110 120 130 140 150
LGAAFLQVAC WMITGERQAA KIRSNYLKTI LRQDIGFFDV ETNTGEVVGR
160 170 180 190 200
MSGDTVHIQD AMGEKVGKFI QLVSTFVGGF ALAFAKGWLL TLVMLTSIPF
210 220 230 240 250
LAMAGAAMAL LVTRASSRGQ AAYAKAATVV EQTIGSIRTV ASFTGEKQAI
260 270 280 290 300
NSYKKYITSA YKSSIQQGFS TGLGLGVMIY VFFSSYALAI WFGGKMILEK
310 320 330 340 350
GYTGGSVINV IIIVVAGSMS LGQTSPCVTA FAAGQAAAYK MFETIKRKPL
360 370 380 390 400
IDAYDVNGKV LGDIRGDIEL KDVHFSYPAR PDEEIFDGFS LFIPSGATAA
410 420 430 440 450
LVGESGSGKS TVINLIERFY DPKAGEVLID GINLKEFQLK WIRSKIGLVC
460 470 480 490 500
QEPVLFSSSI MENIAYGKEN ATLQEIKVAT ELANAAKFIN NLPQGLDTKV
510 520 530 540 550
GEHGTQLSGG QKQRIAIARA ILKDPRVLLL DEATSALDTE SERVVQEALD
560 570 580 590 600
RVMVNRTTVV VAHRLSTVRN ADMIAVIHSG KMVEKGSHSE LLKDSVGAYS
610 620 630 640 650
QLIRCQEINK GHDAKPSDMA SGSSFRNSNL NISREGSVIS GGTSSFGNSS
660 670 680 690 700
RHHSLNVLGL FAGLDLGSGS QRVGQEETGT TSQEPLRKVS LTRIAALNKP
710 720 730 740 750
EIPVLLLGTV VAAINGAIFP LFGILISRVI EAFFKPADQL KKDSRFWAII
760 770 780 790 800
FVALGVTSLI VSPSQMYLFA VAGGKLIRRI QSMCFEKAVH MEVSWFDEPE
810 820 830 840 850
NSSGTMGARL STDAALIRAL VGDALSLAVQ NAASAASGLI IAFTASWELA
860 870 880 890 900
LIILVMLPLI GINGFLQVKF MKGFSADAKS KYEEASQVAN DAVGSIRTVA
910 920 930 940 950
SFCAEEKVMQ MYNKQCEGPI KDGVKQGFIS GLGFGFSFFI LFCVYATSFY
960 970 980 990 1000
AAARLVEDGK TTFIDVFQVF FALTMAAIGI SQSSTFAPDS SKAKVAAASI
1010 1020 1030 1040 1050
FAIIDRKSKI DSSDETGTVL ENVKGDIELR HLSFTYPARP GIQIFRDLCL
1060 1070 1080 1090 1100
TIRAGKTVAL VGESGSGKST VISLLQRFYD PDSGQITLDG VELKKLQLKW
1110 1120 1130 1140 1150
LRQQMGLVGQ EPVLFNDTIR ANIAYGKGSE EAATESEIIA AAELANAHKF
1160 1170 1180 1190 1200
ISSIQQGYDT VVGEKGIQLS GGQKQRVAIA RAIVKEPKIL LLDEATSALD
1210 1220 1230 1240 1250
AESERLVQDA LDRVIVNRTT VVVAHRLSTI KNADVIAIVK NGVIAENGTH
1260 1270
ETLIKIDGGV YASLVQLHMT ASN
Length:1,273
Mass (Da):136,774
Last modified:October 14, 2008 - v2
Checksum:i1CCA06FB3D472241
GO

Sequence cautioni

The sequence AAG10627 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AK228647 differs from that shown. Reason: Frameshift at positions 105 and 420.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279I → T in AK228647 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022521 Genomic DNA. Translation: AAG10627.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE27441.1.
AK228647 mRNA. No translation available.
PIRiF86155.
RefSeqiNP_171754.1. NM_100134.4.
UniGeneiAt.42595.

Genome annotation databases

EnsemblPlantsiAT1G02530.1; AT1G02530.1; AT1G02530.
GeneIDi839282.
GrameneiAT1G02530.1; AT1G02530.1; AT1G02530.
KEGGiath:AT1G02530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022521 Genomic DNA. Translation: AAG10627.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE27441.1.
AK228647 mRNA. No translation available.
PIRiF86155.
RefSeqiNP_171754.1. NM_100134.4.
UniGeneiAt.42595.

3D structure databases

ProteinModelPortaliQ9FWX8.
SMRiQ9FWX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24517. 1 interactor.
STRINGi3702.AT1G02530.1.

Proteomic databases

PaxDbiQ9FWX8.
PRIDEiQ9FWX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02530.1; AT1G02530.1; AT1G02530.
GeneIDi839282.
GrameneiAT1G02530.1; AT1G02530.1; AT1G02530.
KEGGiath:AT1G02530.

Organism-specific databases

TAIRiAT1G02530.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
InParanoidiQ9FWX8.
KOiK05658.
OMAiTIIFADI.
OrthoDBiEOG093600JM.
PhylomeDBiQ9FWX8.

Enzyme and pathway databases

BioCyciARA:AT1G02530-MONOMER.

Miscellaneous databases

PROiQ9FWX8.

Gene expression databases

GenevisibleiQ9FWX8. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAB12B_ARATH
AccessioniPrimary (citable) accession number: Q9FWX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 14, 2008
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.