Reviewed, UniProtKB/Swiss-Prot Q9FWX8 (AB12B_ARATH)

Names and origin

Protein names

Recommended name:
    ABC transporter B family member 12
      Short name=ABC transporter ABCB.12
      Short name=AtABCB12
Alternative name(s):
    Multidrug resistance protein 16
    P-glycoprotein 12

Gene names

Name:	ABCB12
Synonyms:	MDR16, PGP12
Ordered Locus Names:	At1g02530
ORF Names:	T14P4.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Sequence caution	The sequence AK228647 differs from that shown. Reason: Frameshift at positions 105 and 420.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1273	1273	ABC transporter B family member 12		PRO_0000227927
Regions
	Transmembrane	42 – 62	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	169 – 189	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	272 – 292	21	Potential
	Transmembrane	301 – 321	21	Potential
	Transmembrane	706 – 726	21	Potential
	Transmembrane	746 – 766	21	Potential
	Transmembrane	840 – 860	21	Potential
	Transmembrane	927 – 947	21	Potential
	Transmembrane	961 – 981	21	Potential
	Domain	45 – 333	289	ABC transmembrane type-1 1
	Domain	368 – 604	237	ABC transporter 1
	Domain	705 – 992	288	ABC transmembrane type-1 2
	Domain	1027 – 1266	240	ABC transporter 2
	Nucleotide binding	403 – 410	8	ATP 1 Potential
	Nucleotide binding	1062 – 1069	8	ATP 2 Potential
Amino acid modifications
	Glycosylation	470	1	N-linked (GlcNAc...) Potential
	Glycosylation	555	1	N-linked (GlcNAc...) Potential
	Glycosylation	631	1	N-linked (GlcNAc...) Potential
	Glycosylation	648	1	N-linked (GlcNAc...) Potential
	Glycosylation	801	1	N-linked (GlcNAc...) Potential
	Glycosylation	1116	1	N-linked (GlcNAc...) Potential
	Glycosylation	1217	1	N-linked (GlcNAc...) Potential
	Glycosylation	1247	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	279	1	I → T in AK228647. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9FWX8-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 1CCA06FB3D472241	FASTA	1,273	136,774
	10 20 30 40 50 60 MNRDGAGEGD SVSHEHSTSK TDEKAKTVPL YKLFAFADSF DVFLMICGSL GAIGNGVCLP 70 80 90 100 110 120 LMTLLFGDLI DSFGKNQNNK DIVDVVSKVC LKFVYLGLGR LGAAFLQVAC WMITGERQAA 130 140 150 160 170 180 KIRSNYLKTI LRQDIGFFDV ETNTGEVVGR MSGDTVHIQD AMGEKVGKFI QLVSTFVGGF 190 200 210 220 230 240 ALAFAKGWLL TLVMLTSIPF LAMAGAAMAL LVTRASSRGQ AAYAKAATVV EQTIGSIRTV 250 260 270 280 290 300 ASFTGEKQAI NSYKKYITSA YKSSIQQGFS TGLGLGVMIY VFFSSYALAI WFGGKMILEK 310 320 330 340 350 360 GYTGGSVINV IIIVVAGSMS LGQTSPCVTA FAAGQAAAYK MFETIKRKPL IDAYDVNGKV 370 380 390 400 410 420 LGDIRGDIEL KDVHFSYPAR PDEEIFDGFS LFIPSGATAA LVGESGSGKS TVINLIERFY 430 440 450 460 470 480 DPKAGEVLID GINLKEFQLK WIRSKIGLVC QEPVLFSSSI MENIAYGKEN ATLQEIKVAT 490 500 510 520 530 540 ELANAAKFIN NLPQGLDTKV GEHGTQLSGG QKQRIAIARA ILKDPRVLLL DEATSALDTE 550 560 570 580 590 600 SERVVQEALD RVMVNRTTVV VAHRLSTVRN ADMIAVIHSG KMVEKGSHSE LLKDSVGAYS 610 620 630 640 650 660 QLIRCQEINK GHDAKPSDMA SGSSFRNSNL NISREGSVIS GGTSSFGNSS RHHSLNVLGL 670 680 690 700 710 720 FAGLDLGSGS QRVGQEETGT TSQEPLRKVS LTRIAALNKP EIPVLLLGTV VAAINGAIFP 730 740 750 760 770 780 LFGILISRVI EAFFKPADQL KKDSRFWAII FVALGVTSLI VSPSQMYLFA VAGGKLIRRI 790 800 810 820 830 840 QSMCFEKAVH MEVSWFDEPE NSSGTMGARL STDAALIRAL VGDALSLAVQ NAASAASGLI 850 860 870 880 890 900 IAFTASWELA LIILVMLPLI GINGFLQVKF MKGFSADAKS KYEEASQVAN DAVGSIRTVA 910 920 930 940 950 960 SFCAEEKVMQ MYNKQCEGPI KDGVKQGFIS GLGFGFSFFI LFCVYATSFY AAARLVEDGK 970 980 990 1000 1010 1020 TTFIDVFQVF FALTMAAIGI SQSSTFAPDS SKAKVAAASI FAIIDRKSKI DSSDETGTVL 1030 1040 1050 1060 1070 1080 ENVKGDIELR HLSFTYPARP GIQIFRDLCL TIRAGKTVAL VGESGSGKST VISLLQRFYD 1090 1100 1110 1120 1130 1140 PDSGQITLDG VELKKLQLKW LRQQMGLVGQ EPVLFNDTIR ANIAYGKGSE EAATESEIIA 1150 1160 1170 1180 1190 1200 AAELANAHKF ISSIQQGYDT VVGEKGIQLS GGQKQRVAIA RAIVKEPKIL LLDEATSALD 1210 1220 1230 1240 1250 1260 AESERLVQDA LDRVIVNRTT VVVAHRLSTI KNADVIAIVK NGVIAENGTH ETLIKIDGGV 1270 YASLVQLHMT ASN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC022521 Genomic DNA. Translation: AAG10627.1. Different initiation. AK228647 mRNA. No translation available.
IPI	IPI00526168.
PIR	F86155.
RefSeq	NP_171754.1.
UniGene	At.42595
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	839282.
GenomeReviews	Gene locus AT1G02530 in contig CT485782_GR.
KEGG	ath:AT1G02530.
NMPDR	fig|3702.1.peg.374.
Organism-specific databases
TAIR	At1g02530.
Phylogenomic databases
OMA	ENGTHET
Gene expression databases
Genevestigator	Q9FWX8.
GermOnline	AT1G02530. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011527. ABC_TM_1. IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB12B_ARATH

Accession

Primary (citable) accession number: Q9FWX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	October 14, 2008
Last modified:	November 24, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)