Q9FWX6 (KCO4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outward-rectifying potassium channel 4
Short name=AtKCO4

Alternative name(s):
Two-pore potassium channel 4
Short name=AtTPK4

Gene names

Name:	KCO4
Synonyms:	TPK4
Ordered Locus Names:	At1g02510
ORF Names:	T14P4.16

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outward rectifying potassium channel. Blocked by barium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Predominantly expressed in pollen. Ref.1
Domain	Each of the two pore-forming region (also called P-domain or P-loop) is enclosed by two transmembrane segments (2P/4TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.7) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ionic channel Potassium channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Outward-rectifying potassium channel 4		PRO_0000101778
Regions
	Topological domain	1 – 31	31	Cytoplasmic Potential
	Transmembrane	32 – 52	21	Helical; Potential
	Intramembrane	70 – 89	20	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	93 – 113	21	Helical; Potential
	Topological domain	114 – 156	43	Cytoplasmic Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Intramembrane	184 – 203	20	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	211 – 231	21	Helical; Potential
	Topological domain	232 – 284	53	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FWX6 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: AF54B529AE1BBB91	FASTA	284	32,061
	10 20 30 40 50 60 MEEENLLNEN LLHPNESSPE ETQVTTVSKS KWTILVLAMI LLLVYLTFGV CTYSFFRDQF 70 80 90 100 110 120 SGTETNLFVD AFYFSIVTFS TVGYGDIVPS TSTTKILTIV LVSTGVVFLD YLLNRVVSHV 130 140 150 160 170 180 LSLQENAILD RINKTRNRAI RDHIAEDGKI RLKWKLCLAF CAVGLCVGSG ALFLHVFERL 190 200 210 220 230 240 DWLDSVYLSV ISVTTVGYGD KTFKTVEGRG FAVFWLLLST IAMATLFLYL AEMRIDRTTV 250 260 270 280 MKLPPSESEF IVFKLRESGR ISEDDIKQIV REFENLEEVP SSGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AtTPK4, an Arabidopsis tandem-pore K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+-dependent manner." Becker D., Geiger D., Dunkel M., Roller A., Bertl A., Latz A., Carpaneto A., Dietrich P., Roelfsema M.R., Voelker C., Schmidt D., Mueller-Roeber B., Czempinski K., Hedrich R. Proc. Natl. Acad. Sci. U.S.A. 101:15621-15626(2004) [PubMed: 15505206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Phylogenetic relationships within cation transporter families of Arabidopsis." Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H., Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F., Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L. Plant Physiol. 126:1646-1667(2001) [PubMed: 11500563] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY258073 mRNA. Translation: AAP82009.1. AC022521 Genomic DNA. Translation: AAG10638.1. CP002684 Genomic DNA. Translation: AEE27439.1.
IPI	IPI00520180.
PIR	D86155.
RefSeq	NP_171752.1. NM_100132.2.
UniGene	At.42597.
3D structure databases
ProteinModelPortal	Q9FWX6.
SMR	Q9FWX6. Positions 34-119, 180-238.
ModBase	Search...
Proteomic databases
PRIDE	Q9FWX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02510.1; AT1G02510.1; AT1G02510.
GeneID	837846.
GenomeReviews	Gene locus AT1G02510 in contig CT485782_GR.
KEGG	ath:AT1G02510.
Organism-specific databases
GeneFarm	2721. 258.
TAIR	At1g02510.
Phylogenomic databases
GeneTree	EPGT00070000028806.
InParanoid	Q9FWX6.
OMA	ESEFIVF.
PhylomeDB	Q9FWX6.
Gene expression databases
Genevestigator	Q9FWX6.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR013099. Ion_trans_2. [Graphical view]
KO	K05389.
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL.
ProtoNet	Search...

Entry information

Entry name

KCO4_ARATH

Accession

Primary (citable) accession number: Q9FWX6
Secondary accession number(s): Q6X308

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	February 8, 2011
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program