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Q9FWT2

- UBC16_ARATH

UniProt

Q9FWT2 - UBC16_ARATH

Protein

Probable ubiquitin-conjugating enzyme E2 16

Gene

UBC16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei99 – 991Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G75440-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin-conjugating enzyme E2 16 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 16
    Gene namesi
    Name:UBC16
    Ordered Locus Names:At1g75440
    ORF Names:F1B16.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G75440.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Probable ubiquitin-conjugating enzyme E2 16PRO_0000345182Add
    BLAST

    Proteomic databases

    PaxDbiQ9FWT2.
    PRIDEiQ9FWT2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FWT2.

    Interactioni

    Protein-protein interaction databases

    BioGridi29099. 3 interactions.
    IntActiQ9FWT2. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FWT2.
    SMRiQ9FWT2. Positions 19-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233453.
    InParanoidiQ9FWT2.
    KOiK10688.
    OMAiTGNTKNE.
    PhylomeDBiQ9FWT2.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FWT2-1 [UniParc]FASTAAdd to Basket

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    MSSSGAPSRK TLSKIATNRL QKELVEWQMN PPTGFKHKVT DNLQRWIIEV    50
    IGAPGTLYAN DTYQLQVDFP EHYPMESPQV IFLHPAPLHP HIYSNGHICL 100
    DILYDSWSPA MTVSSICISI LSMLSSSTEK QRPTDNDRYV KNCKNGRSPK 150
    ETRWWFHDDK V 161
    Length:161
    Mass (Da):18,485
    Last modified:March 1, 2001 - v1
    Checksum:iEC527C112BA88D89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111T → V in AAC39325. 1 PublicationCurated
    Sequence conflicti81 – 844IFLH → SFSS in AAC39325. 1 PublicationCurated
    Sequence conflicti88 – 881L → S in AAC39325. 1 PublicationCurated
    Sequence conflicti129 – 1291E → A in AAC39325. 1 PublicationCurated
    Sequence conflicti146 – 1494GRSP → EDSSA in AAC39325. 1 PublicationCurated
    Sequence conflicti159 – 1591D → N in AAC39325. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF028339 Genomic DNA. Translation: AAC39325.1.
    DQ027030 mRNA. Translation: AAY44856.1.
    AC023754 Genomic DNA. Translation: AAG13066.1.
    CP002684 Genomic DNA. Translation: AEE35720.1.
    AY065459 mRNA. Translation: AAL38900.1.
    AY091226 mRNA. Translation: AAM14165.1.
    PIRiA96785.
    RefSeqiNP_565110.1. NM_106198.4.
    UniGeneiAt.16253.
    At.28601.

    Genome annotation databases

    EnsemblPlantsiAT1G75440.1; AT1G75440.1; AT1G75440.
    GeneIDi843880.
    KEGGiath:AT1G75440.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF028339 Genomic DNA. Translation: AAC39325.1 .
    DQ027030 mRNA. Translation: AAY44856.1 .
    AC023754 Genomic DNA. Translation: AAG13066.1 .
    CP002684 Genomic DNA. Translation: AEE35720.1 .
    AY065459 mRNA. Translation: AAL38900.1 .
    AY091226 mRNA. Translation: AAM14165.1 .
    PIRi A96785.
    RefSeqi NP_565110.1. NM_106198.4.
    UniGenei At.16253.
    At.28601.

    3D structure databases

    ProteinModelPortali Q9FWT2.
    SMRi Q9FWT2. Positions 19-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29099. 3 interactions.
    IntActi Q9FWT2. 3 interactions.

    Proteomic databases

    PaxDbi Q9FWT2.
    PRIDEi Q9FWT2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G75440.1 ; AT1G75440.1 ; AT1G75440 .
    GeneIDi 843880.
    KEGGi ath:AT1G75440.

    Organism-specific databases

    TAIRi AT1G75440.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233453.
    InParanoidi Q9FWT2.
    KOi K10688.
    OMAi TGNTKNE.
    PhylomeDBi Q9FWT2.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT1G75440-MONOMER.

    Gene expression databases

    Genevestigatori Q9FWT2.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
      Yan N., Doelling J., Vierstra R.D.
      Plant Gene Register PGR97-174
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBC16_ARATH
    AccessioniPrimary (citable) accession number: Q9FWT2
    Secondary accession number(s): O48953
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3