Reviewed, UniProtKB/Swiss-Prot Q9FWT2 (UBC16_ARATH)

Names and origin

Protein names

Recommended name:
    Probable ubiquitin carrier protein E2 16
    EC=6.3.2.19

Gene names

Name:	UBC16
Ordered Locus Names:	At1g75440
ORF Names:	F1B16.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Probable ubiquitin carrier protein E2 16		PRO_0000345182
Sites
	Active site	99	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	11	1	T → V in AAC39325. Ref.1
	Sequence conflict	81 – 84	4	IFLH → SFSS in AAC39325. Ref.1
	Sequence conflict	88	1	L → S in AAC39325. Ref.1
	Sequence conflict	129	1	E → A in AAC39325. Ref.1
	Sequence conflict	146 – 149	4	GRSP → EDSSA in AAC39325. Ref.1
	Sequence conflict	159	1	D → N in AAC39325. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FWT2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: EC527C112BA88D89	FASTA	161	18,485
	10 20 30 40 50 60 MSSSGAPSRK TLSKIATNRL QKELVEWQMN PPTGFKHKVT DNLQRWIIEV IGAPGTLYAN 70 80 90 100 110 120 DTYQLQVDFP EHYPMESPQV IFLHPAPLHP HIYSNGHICL DILYDSWSPA MTVSSICISI 130 140 150 160 LSMLSSSTEK QRPTDNDRYV KNCKNGRSPK ETRWWFHDDK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana." Yan N., Doelling J., Vierstra R.D. Plant Gene Register PGR97-174 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF028339 Genomic DNA. Translation: AAC39325.1. DQ027030 mRNA. Translation: AAY44856.1. AC023754 Genomic DNA. Translation: AAG13066.1. AY065459 mRNA. Translation: AAL38900.1. AY091226 mRNA. Translation: AAM14165.1.
IPI	IPI00523061.
PIR	A96785.
RefSeq	NP_565110.1.
UniGene	At.28601
3D structure databases
HSSP	HSSP built from PDB template 1I7K based on UniProtKB O00762.
ModBase	Search...
Genome annotation databases
GeneID	843880.
GenomeReviews	Gene locus AT1G75440 in contig CT485782_GR.
KEGG	ath:AT1G75440.
NMPDR	fig|3702.1.peg.7065.
Organism-specific databases
TAIR	At1g75440.
Phylogenomic databases
OMA	TCSKNPK.
Gene expression databases
ArrayExpress	Q9FWT2.
Genevestigator	Q9FWT2.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC16_ARATH

Accession

Primary (citable) accession number: Q9FWT2
Secondary accession number(s): O48953

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)