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Q9FWT2

- UBC16_ARATH

UniProt

Q9FWT2 - UBC16_ARATH

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Protein

Probable ubiquitin-conjugating enzyme E2 16

Gene

UBC16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G75440-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 16 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 16
Gene namesi
Name:UBC16
Ordered Locus Names:At1g75440
ORF Names:F1B16.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G75440.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Probable ubiquitin-conjugating enzyme E2 16PRO_0000345182Add
BLAST

Proteomic databases

PaxDbiQ9FWT2.
PRIDEiQ9FWT2.

Expressioni

Gene expression databases

GenevestigatoriQ9FWT2.

Interactioni

Protein-protein interaction databases

BioGridi29099. 3 interactions.
IntActiQ9FWT2. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9FWT2.
SMRiQ9FWT2. Positions 16-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233453.
InParanoidiQ9FWT2.
KOiK10688.
OMAiTGNTKNE.
PhylomeDBiQ9FWT2.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FWT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSGAPSRK TLSKIATNRL QKELVEWQMN PPTGFKHKVT DNLQRWIIEV
60 70 80 90 100
IGAPGTLYAN DTYQLQVDFP EHYPMESPQV IFLHPAPLHP HIYSNGHICL
110 120 130 140 150
DILYDSWSPA MTVSSICISI LSMLSSSTEK QRPTDNDRYV KNCKNGRSPK
160
ETRWWFHDDK V
Length:161
Mass (Da):18,485
Last modified:March 1, 2001 - v1
Checksum:iEC527C112BA88D89
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111T → V in AAC39325. 1 PublicationCurated
Sequence conflicti81 – 844IFLH → SFSS in AAC39325. 1 PublicationCurated
Sequence conflicti88 – 881L → S in AAC39325. 1 PublicationCurated
Sequence conflicti129 – 1291E → A in AAC39325. 1 PublicationCurated
Sequence conflicti146 – 1494GRSP → EDSSA in AAC39325. 1 PublicationCurated
Sequence conflicti159 – 1591D → N in AAC39325. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF028339 Genomic DNA. Translation: AAC39325.1.
DQ027030 mRNA. Translation: AAY44856.1.
AC023754 Genomic DNA. Translation: AAG13066.1.
CP002684 Genomic DNA. Translation: AEE35720.1.
AY065459 mRNA. Translation: AAL38900.1.
AY091226 mRNA. Translation: AAM14165.1.
PIRiA96785.
RefSeqiNP_565110.1. NM_106198.4.
UniGeneiAt.16253.
At.28601.

Genome annotation databases

EnsemblPlantsiAT1G75440.1; AT1G75440.1; AT1G75440.
GeneIDi843880.
KEGGiath:AT1G75440.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF028339 Genomic DNA. Translation: AAC39325.1 .
DQ027030 mRNA. Translation: AAY44856.1 .
AC023754 Genomic DNA. Translation: AAG13066.1 .
CP002684 Genomic DNA. Translation: AEE35720.1 .
AY065459 mRNA. Translation: AAL38900.1 .
AY091226 mRNA. Translation: AAM14165.1 .
PIRi A96785.
RefSeqi NP_565110.1. NM_106198.4.
UniGenei At.16253.
At.28601.

3D structure databases

ProteinModelPortali Q9FWT2.
SMRi Q9FWT2. Positions 16-156.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29099. 3 interactions.
IntActi Q9FWT2. 3 interactions.

Proteomic databases

PaxDbi Q9FWT2.
PRIDEi Q9FWT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G75440.1 ; AT1G75440.1 ; AT1G75440 .
GeneIDi 843880.
KEGGi ath:AT1G75440.

Organism-specific databases

TAIRi AT1G75440.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233453.
InParanoidi Q9FWT2.
KOi K10688.
OMAi TGNTKNE.
PhylomeDBi Q9FWT2.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G75440-MONOMER.

Gene expression databases

Genevestigatori Q9FWT2.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
    Yan N., Doelling J., Vierstra R.D.
    Plant Gene Register PGR97-174
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBC16_ARATH
AccessioniPrimary (citable) accession number: Q9FWT2
Secondary accession number(s): O48953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3