Reviewed, UniProtKB/Swiss-Prot Q9FWR2 (AVPX_ARATH)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized membrane proton pump 3
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase 3
      Short name=H(+)-PPase 3
    AVP1-like protein 2

Gene names

Name:	AVPL2
Ordered Locus Names:	At1g16780
ORF Names:	F17F16.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Subunit structure	Monomer By similarity.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]
Caution	It is uncertain whether Met-1, Met-2 or Met-3 is the initiator.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from direct assay. Source: TAIR
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 802	802	Pyrophosphate-energized membrane proton pump 3		PRO_0000217041
Regions
	Transmembrane	41 – 61	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	118 – 138	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	206 – 226	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	348 – 368	21	Potential
	Transmembrane	386 – 406	21	Potential
	Transmembrane	421 – 441	21	Potential
	Transmembrane	456 – 476	21	Potential
	Transmembrane	483 – 503	21	Potential
	Transmembrane	511 – 531	21	Potential
	Transmembrane	577 – 597	21	Potential
	Transmembrane	615 – 635	21	Potential
	Transmembrane	686 – 706	21	Potential
	Transmembrane	716 – 736	21	Potential
	Transmembrane	782 – 802	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FWR2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E3CFD70DAF219880	FASTA	802	85,349
	10 20 30 40 50 60 MMMDEDVEQA TLVSYSDKPR TFPDMRSKTY SPLIIRILRN LNVRALSVLL LLSFGGIFYM 70 80 90 100 110 120 GARTSPIIVF VFVVCIISFM LSVYLTKWVL AKDEGPPEMV QISDAIRDGA EGFLRTQYGT 130 140 150 160 170 180 ISKMAFLLAF VILCIYLFRN LTPQQEASGL GRTMSAYITV AAFLLGALCS GIAGYVGMWV 190 200 210 220 230 240 SVRANVRVSS AARRSAREAL QIAVRAGGFS ALVVVGMAVI GIAILYSTFY VWLDVDSPGS 250 260 270 280 290 300 MKVTDLPLLL VGYGFGASFV ALFAQLGGGI YTKGADVGAD LVGKVEHGIP EDDPRNPAVI 310 320 330 340 350 360 ADLVGDNVGD CAARGADLFE SIAAEIISAM ILGGTMAQKC KIEDPSGFIL FPLVVHSFDL 370 380 390 400 410 420 VISSIGILSI KGTRNASVKS PVEDPMVVLQ KGYSLTIILA VLTFGASTRW LLYTEQAPSA 430 440 450 460 470 480 WLNFFMCGLV GIITAYVFVW ISRYYTDYKY EPVRTLALAS STGHGTNIIA GVSLGLESTA 490 500 510 520 530 540 LPVLVISVAI ISAFWLGNTS GLIDEKGNPT GGLFGTAVAT MGMLSTAAYV LTMDMFGPIA 550 560 570 580 590 600 DNAGGIVEMS QQPESVREIT DVLDAVGNTT KATTKGFAIG SAALASFLLF SAYMDEVSAF 610 620 630 640 650 660 ANVSFKEVDI AIPEVFIGGL LGAMLIFLFS AWACAAVGRT AQEVVNEVRR QFIERPGIMD 670 680 690 700 710 720 YKEKPDYGRC VAIVASSALR EMIKPGALAI ISPIAVGFVF RILGYYTGQP LLGAKVVAAM 730 740 750 760 770 780 LMFATVCGIL MALFLNTAGG AWDNAKKYIE TGALGGKGSD SHKAAVTGDT VGDPFKDTAG 790 800 PSIHVLIKML ATITLVMAPI FL

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC026237 Genomic DNA. Translation: AAG09080.1.
IPI	IPI00536553.
PIR	C86303.
RefSeq	NP_173122.1.
UniGene	At.51639
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	838249.
GenomeReviews	Gene locus AT1G16780 in contig CT485782_GR.
KEGG	ath:AT1G16780.
NMPDR	fig|3702.1.peg.1993.
Organism-specific databases
GeneFarm	5161. 491.
TAIR	At1g16780.
Phylogenomic databases
OMA	IMSISET.
Enzyme and pathway databases
BRENDA	3.6.1.1. 302.
Gene expression databases
Genevestigator	Q9FWR2.
GermOnline	AT1G16780. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AVPX_ARATH

Accession

Primary (citable) accession number: Q9FWR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)