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Protein

6-phosphogluconate dehydrogenase, decarboxylating 3

Gene

At3g02360

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 3 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 2 (At3g49360), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700)
  3. 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071NADPBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Active sitei188 – 1881Proton acceptorBy similarity
Active sitei195 – 1951Proton donorBy similarity
Binding sitei196 – 1961SubstrateBy similarity
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Binding sitei293 – 2931SubstrateBy similarity
Binding sitei456 – 4561Substrate; shared with dimeric partnerBy similarity
Binding sitei462 – 4621Substrate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NADPBy similarity
Nucleotide bindingi35 – 373NADPBy similarity
Nucleotide bindingi79 – 813NADPBy similarity

GO - Molecular functioni

GO - Biological processi

  • D-gluconate metabolic process Source: UniProtKB-KW
  • pentose-phosphate shunt Source: UniProtKB-UniPathway
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization, Pentose shunt

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT3G02360-MONOMER.
ARA:GQT-1389-MONOMER.
ReactomeiR-ATH-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00410.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating 3 (EC:1.1.1.44)
Gene namesi
Ordered Locus Names:At3g02360
ORF Names:F11A12.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G02360.

Subcellular locationi

GO - Cellular componenti

  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4864866-phosphogluconate dehydrogenase, decarboxylating 3PRO_0000421100Add
BLAST

Proteomic databases

PaxDbiQ9FWA3.
PRIDEiQ9FWA3.

PTM databases

iPTMnetiQ9FWA3.

Expressioni

Gene expression databases

GenevisibleiQ9FWA3. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi6496. 3 interactions.
STRINGi3702.AT3G02360.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FWA3.
SMRiQ9FWA3. Positions 7-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1353Substrate bindingBy similarity
Regioni191 – 1922Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2653. Eukaryota.
COG0362. LUCA.
HOGENOMiHOG000255147.
InParanoidiQ9FWA3.
KOiK00033.
OMAiAFHTEWF.
PhylomeDBiQ9FWA3.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR012284. 6PGD_dom_3.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_Gnd/GntZ.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR006183. Pgluconate_DH.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SMARTiSM01350. 6PGD. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FWA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVQPTRIGL AGLAVMGQNL ALNIAEKGFP ISVYNRTTSK VDETVERAKK
60 70 80 90 100
EGNLPLYGFH DPESFVKSIQ KPRVIIMLVK AGSPVDQTIK TLSAYLEKGD
110 120 130 140 150
CIVDGGNEWY ENTERREKAV AENGFLYLGM GVSGGEEGAR NGPSMMPGGS
160 170 180 190 200
YEAYKNIEDI VLKVAAQVRD SGPCVTYIGK GGSGNFVKMV HNGIEYGDMQ
210 220 230 240 250
LIAEAYDVLK SVGKLSNEEL HSVFSDWNKG ELESFLVEIT ADIFGIKDDK
260 270 280 290 300
GDGHLVDKVL DKTGMKGTGK WTVQQAAELS VPAPTIESSL DARFLSGLKD
310 320 330 340 350
ERVQAAKVFK AGGFGDILTD QKVDKKQLVD DVRKALYASK ICSYAQGMNL
360 370 380 390 400
IRAKSIEKGW GLKLGELARI WKGGCIIRAI FLDRIKQAYD RNAELANLLV
410 420 430 440 450
DPEFAKEIIE RQSAWRRVVC LAINSGISTP GMSASLAYFD SYRRERLPAN
460 470 480
LVQAQRDYFG AHTYERTDVE GSFHTEWFKI ARQSKI
Length:486
Mass (Da):53,577
Last modified:March 1, 2001 - v1
Checksum:i894395559DAEB020
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068900 Genomic DNA. Translation: AAG12595.1.
CP002686 Genomic DNA. Translation: AEE73796.1.
CP002686 Genomic DNA. Translation: AEE73797.1.
AF424591 mRNA. Translation: AAL11585.1.
BT002299 mRNA. Translation: AAN73296.1.
AK227160 mRNA. Translation: BAE99202.1.
AY087341 mRNA. Translation: AAM64891.1.
RefSeqiNP_186885.1. NM_111103.5.
NP_850502.1. NM_180171.2.
UniGeneiAt.16821.

Genome annotation databases

EnsemblPlantsiAT3G02360.1; AT3G02360.1; AT3G02360.
AT3G02360.2; AT3G02360.2; AT3G02360.
GeneIDi821163.
GrameneiAT3G02360.1; AT3G02360.1; AT3G02360.
AT3G02360.2; AT3G02360.2; AT3G02360.
KEGGiath:AT3G02360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068900 Genomic DNA. Translation: AAG12595.1.
CP002686 Genomic DNA. Translation: AEE73796.1.
CP002686 Genomic DNA. Translation: AEE73797.1.
AF424591 mRNA. Translation: AAL11585.1.
BT002299 mRNA. Translation: AAN73296.1.
AK227160 mRNA. Translation: BAE99202.1.
AY087341 mRNA. Translation: AAM64891.1.
RefSeqiNP_186885.1. NM_111103.5.
NP_850502.1. NM_180171.2.
UniGeneiAt.16821.

3D structure databases

ProteinModelPortaliQ9FWA3.
SMRiQ9FWA3. Positions 7-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6496. 3 interactions.
STRINGi3702.AT3G02360.1.

PTM databases

iPTMnetiQ9FWA3.

Proteomic databases

PaxDbiQ9FWA3.
PRIDEiQ9FWA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G02360.1; AT3G02360.1; AT3G02360.
AT3G02360.2; AT3G02360.2; AT3G02360.
GeneIDi821163.
GrameneiAT3G02360.1; AT3G02360.1; AT3G02360.
AT3G02360.2; AT3G02360.2; AT3G02360.
KEGGiath:AT3G02360.

Organism-specific databases

TAIRiAT3G02360.

Phylogenomic databases

eggNOGiKOG2653. Eukaryota.
COG0362. LUCA.
HOGENOMiHOG000255147.
InParanoidiQ9FWA3.
KOiK00033.
OMAiAFHTEWF.
PhylomeDBiQ9FWA3.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00410.
BioCyciARA:AT3G02360-MONOMER.
ARA:GQT-1389-MONOMER.
ReactomeiR-ATH-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

PROiQ9FWA3.

Gene expression databases

GenevisibleiQ9FWA3. AT.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR012284. 6PGD_dom_3.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_Gnd/GntZ.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR006183. Pgluconate_DH.
[Graphical view]
PfamiPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000109. 6PGD. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SMARTiSM01350. 6PGD. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The oxidative pentose phosphate pathway: structure and organisation."
    Kruger N.J., von Schaewen A.
    Curr. Opin. Plant Biol. 6:236-246(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. Cited for: GENE FAMILY.

Entry informationi

Entry namei6GPD3_ARATH
AccessioniPrimary (citable) accession number: Q9FWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.