Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

COP9 signalosome complex subunit 5a

Gene

CSN5A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.By similarity2 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Zinc; catalyticBy similarity1
Metal bindingi144Zinc; catalyticBy similarity1
Metal bindingi155Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • COP9 signalosome assembly Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of photomorphogenesis Source: TAIR
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • positive regulation of protein catabolic process Source: TAIR
  • protein deneddylation Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • regulation of L-ascorbic acid biosynthetic process Source: TAIR
  • response to auxin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Phytochrome signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Protein family/group databases

MEROPSiM67.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 5a (EC:3.4.-.-)
Short name:
Signalosome subunit 5a
Alternative name(s):
Jun activation domain-binding homolog 2
Gene namesi
Name:CSN5A
Synonyms:AJH2
Ordered Locus Names:At1g71230
ORF Names:F3I17.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G71230.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948421 – 358COP9 signalosome complex subunit 5aAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9FVU9.
PRIDEiQ9FVU9.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.1 Publication

Gene expression databases

GenevisibleiQ9FVU9. AT.

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8. Interacts with itself. In the complex, it is located in the center and probably interacts directly with CSN4 and CSN6A or CSN6B. Also exists as monomeric form.2 Publications

Protein-protein interaction databases

BioGridi28683. 42 interactors.
IntActiQ9FVU9. 59 interactors.
MINTiMINT-8068238.
STRINGi3702.AT1G71230.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FVU9.
SMRiQ9FVU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 168MPNAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 155JAMM motifAdd BLAST14

Domaini

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. CSN5 subfamily.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ9FVU9.
KOiK09613.
OMAiEKPWEND.
OrthoDBiEOG09360E02.
PhylomeDBiQ9FVU9.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FVU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSSSTIAR KTWELENSIL TVDSPDSTSD NIFYYDDTSQ TRFQQEKPWE
60 70 80 90 100
NDPHYFKRVK ISALALLKMV VHARSGGTIE IMGLMQGKTD GDTIIVMDAF
110 120 130 140 150
ALPVEGTETR VNAQDDAYEY MVEYSQTNKL AGRLENVVGW YHSHPGYGCW
160 170 180 190 200
LSGIDVSTQR LNQQHQEPFL AVVIDPTRTV SAGKVEIGAF RTYSKGYKPP
210 220 230 240 250
DEPVSEYQTI PLNKIEDFGV HCKQYYSLDV TYFKSSLDSH LLDLLWNKYW
260 270 280 290 300
VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESHLVQSRFG GVVPSSLHKK
310 320 330 340 350
KEDESQLTKI TRDSAKITVE QVHGLMSQVI KDELFNSMRQ SNNKSPTDSS

DPDPMITY
Length:358
Mass (Da):40,318
Last modified:March 1, 2001 - v1
Checksum:i4890F2D35F6FB410
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160R → T in AAC36343 (PubMed:9811788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087412 mRNA. Translation: AAC36343.1.
AF395061 mRNA. Translation: AAL58104.1.
AC016162 Genomic DNA. Translation: AAG51882.1.
CP002684 Genomic DNA. Translation: AEE35176.1.
AF411778 mRNA. Translation: AAL06468.1.
AY124816 mRNA. Translation: AAM70525.1.
PIRiH96736.
T52042.
RefSeqiNP_177279.1. NM_105792.2.
UniGeneiAt.17922.
At.68950.

Genome annotation databases

EnsemblPlantsiAT1G71230.1; AT1G71230.1; AT1G71230.
GeneIDi843463.
GrameneiAT1G71230.1; AT1G71230.1; AT1G71230.
KEGGiath:AT1G71230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087412 mRNA. Translation: AAC36343.1.
AF395061 mRNA. Translation: AAL58104.1.
AC016162 Genomic DNA. Translation: AAG51882.1.
CP002684 Genomic DNA. Translation: AEE35176.1.
AF411778 mRNA. Translation: AAL06468.1.
AY124816 mRNA. Translation: AAM70525.1.
PIRiH96736.
T52042.
RefSeqiNP_177279.1. NM_105792.2.
UniGeneiAt.17922.
At.68950.

3D structure databases

ProteinModelPortaliQ9FVU9.
SMRiQ9FVU9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28683. 42 interactors.
IntActiQ9FVU9. 59 interactors.
MINTiMINT-8068238.
STRINGi3702.AT1G71230.1.

Protein family/group databases

MEROPSiM67.A02.

Proteomic databases

PaxDbiQ9FVU9.
PRIDEiQ9FVU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71230.1; AT1G71230.1; AT1G71230.
GeneIDi843463.
GrameneiAT1G71230.1; AT1G71230.1; AT1G71230.
KEGGiath:AT1G71230.

Organism-specific databases

TAIRiAT1G71230.

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ9FVU9.
KOiK09613.
OMAiEKPWEND.
OrthoDBiEOG09360E02.
PhylomeDBiQ9FVU9.

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Miscellaneous databases

PROiQ9FVU9.

Gene expression databases

GenevisibleiQ9FVU9. AT.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSN5A_ARATH
AccessioniPrimary (citable) accession number: Q9FVU9
Secondary accession number(s): O82523
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.