Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

COP9 signalosome complex subunit 5b

Gene

CSN5B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Not involved in CSN's deneddylation/derubylation activity (PubMed:15486099, PubMed:17307927). Essential for the structural integrity of the CSN holocomplex (PubMed:17307927).By similarity4 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Zinc; catalyticBy similarity1
Metal bindingi144Zinc; catalyticBy similarity1
Metal bindingi155Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • COP9 signalosome assembly Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of photomorphogenesis Source: TAIR
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • positive regulation of protein catabolic process Source: TAIR
  • protein deneddylation Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • regulation of L-ascorbic acid biosynthetic process Source: TAIR
  • response to auxin Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Metalloprotease, Protease
Biological processPhytochrome signaling pathway
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM67.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 5b (EC:3.4.-.-)
Short name:
Signalosome subunit 5b
Alternative name(s):
Jun activation domain-binding homolog 2
Gene namesi
Name:CSN5B1 Publication
Synonyms:AJH21 Publication, CSN5A1 Publication
Ordered Locus Names:At1g71230Imported
ORF Names:F3I17.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2032288. AT1G71230.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype and no effect on the derubylation of CUL1 (PubMed:15486099, PubMed:17307927). Csn5a and csn5b double mutants are lethal at the seedling stage (PubMed:17307927).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi142H → A: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1
Mutagenesisi144H → A: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1
Mutagenesisi149C → A: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1
Mutagenesisi155D → N: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1
Mutagenesisi175D → E or N: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948421 – 358COP9 signalosome complex subunit 5bAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9FVU9.
PRIDEiQ9FVU9.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.1 Publication

Gene expression databases

GenevisibleiQ9FVU9. AT.

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8 (PubMed:9811788, PubMed:12615944, PubMed:15486099). CSN5A or CSN5B are present within distinct CSN complexes each containing only one copy of CSN5 (PubMed:15486099). Interacts with itself (PubMed:9811788). In the complex, it is located in the center and probably interacts directly with CSN4 and CSN6A or CSN6B (PubMed:9811788, PubMed:12615944). Also exists as monomeric form (PubMed:9811788). Interacts with CYT1 in vitro and in planta (PubMed:23424245).4 Publications

Protein-protein interaction databases

BioGridi28683. 42 interactors.
IntActiQ9FVU9. 59 interactors.
MINTiMINT-8068238.
STRINGi3702.AT1G71230.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FVU9.
SMRiQ9FVU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 168MPNAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 155JAMM motifAdd BLAST14

Domaini

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. CSN5 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ9FVU9.
KOiK09613.
OMAiEKPWEND.
OrthoDBiEOG09360E02.
PhylomeDBiQ9FVU9.

Family and domain databases

InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FVU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSSSTIAR KTWELENSIL TVDSPDSTSD NIFYYDDTSQ TRFQQEKPWE
60 70 80 90 100
NDPHYFKRVK ISALALLKMV VHARSGGTIE IMGLMQGKTD GDTIIVMDAF
110 120 130 140 150
ALPVEGTETR VNAQDDAYEY MVEYSQTNKL AGRLENVVGW YHSHPGYGCW
160 170 180 190 200
LSGIDVSTQR LNQQHQEPFL AVVIDPTRTV SAGKVEIGAF RTYSKGYKPP
210 220 230 240 250
DEPVSEYQTI PLNKIEDFGV HCKQYYSLDV TYFKSSLDSH LLDLLWNKYW
260 270 280 290 300
VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESHLVQSRFG GVVPSSLHKK
310 320 330 340 350
KEDESQLTKI TRDSAKITVE QVHGLMSQVI KDELFNSMRQ SNNKSPTDSS

DPDPMITY
Length:358
Mass (Da):40,318
Last modified:March 1, 2001 - v1
Checksum:i4890F2D35F6FB410
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160R → T in AAC36343 (PubMed:9811788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087412 mRNA. Translation: AAC36343.1.
AF395061 mRNA. Translation: AAL58104.1.
AC016162 Genomic DNA. Translation: AAG51882.1.
CP002684 Genomic DNA. Translation: AEE35176.1.
AF411778 mRNA. Translation: AAL06468.1.
AY124816 mRNA. Translation: AAM70525.1.
PIRiH96736.
T52042.
RefSeqiNP_177279.1. NM_105792.2.
UniGeneiAt.17922.
At.68950.

Genome annotation databases

EnsemblPlantsiAT1G71230.1; AT1G71230.1; AT1G71230.
GeneIDi843463.
GrameneiAT1G71230.1; AT1G71230.1; AT1G71230.
KEGGiath:AT1G71230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087412 mRNA. Translation: AAC36343.1.
AF395061 mRNA. Translation: AAL58104.1.
AC016162 Genomic DNA. Translation: AAG51882.1.
CP002684 Genomic DNA. Translation: AEE35176.1.
AF411778 mRNA. Translation: AAL06468.1.
AY124816 mRNA. Translation: AAM70525.1.
PIRiH96736.
T52042.
RefSeqiNP_177279.1. NM_105792.2.
UniGeneiAt.17922.
At.68950.

3D structure databases

ProteinModelPortaliQ9FVU9.
SMRiQ9FVU9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28683. 42 interactors.
IntActiQ9FVU9. 59 interactors.
MINTiMINT-8068238.
STRINGi3702.AT1G71230.1.

Protein family/group databases

MEROPSiM67.A02.

Proteomic databases

PaxDbiQ9FVU9.
PRIDEiQ9FVU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71230.1; AT1G71230.1; AT1G71230.
GeneIDi843463.
GrameneiAT1G71230.1; AT1G71230.1; AT1G71230.
KEGGiath:AT1G71230.

Organism-specific databases

AraportiAT1G71230.
TAIRilocus:2032288. AT1G71230.

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ9FVU9.
KOiK09613.
OMAiEKPWEND.
OrthoDBiEOG09360E02.
PhylomeDBiQ9FVU9.

Miscellaneous databases

PROiPR:Q9FVU9.

Gene expression databases

GenevisibleiQ9FVU9. AT.

Family and domain databases

InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCSN5B_ARATH
AccessioniPrimary (citable) accession number: Q9FVU9
Secondary accession number(s): O82523
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2001
Last modified: April 12, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.